Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quick detection method for Scylla paramamosain by microsatellite markers from functional genes

A technology of microsatellite markers and pseudo-cave blue crabs, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve the problems of small number of microsatellite markers and limit the development of genetics research, and achieve the amplification of bands clear effect

Inactive Publication Date: 2010-07-28
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the number of microsatellite markers available in Scylla cryptae is very small, and there is no report of type I (markers related to functional genes) microsatellite markers, which severely limits the development of related genetic research.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quick detection method for Scylla paramamosain by microsatellite markers from functional genes
  • Quick detection method for Scylla paramamosain by microsatellite markers from functional genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0020] 1. Extraction of Genomic DNA of Scylla pseudocaveus

[0021] Cut a small amount of muscle tissue (approximately 100-150 mg) from Scylla pseudocavena and place it in 500 μl tissue extract (10 mmol / L Tris-Cl, pH 8.0; 100 mmol / L LEDTA, pH 8.0; 100 mmol / L NaCl; 0.5% SDS) Minced, added proteinase K (final concentration 20mg / ml) and RNase A (final concentration 100μg / ml), mixed well, digested at 55°C for 2-3 hours until clear...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a quick detection method for Scylla paramamosain by microsatellite markers from functional genes, which comprises the following steps: (1) extracting genomic DNA of the Scylla paramamosain; (2) acquiring a gene sequence containing microsatellite repeats according to a functional gene sequence of the Scylla paramamosain in a GeneBank database; (3) designing microsatellite marker primers; (4) performing PCR amplification on the genomic DNA of different geographic population or different individuals in the population of the Scylla paramamosain; and (5) detecting a PCR product by polyacrylamide gel electrophoresis. The detection method has the advantages of quickness, accuracy, sensitivity and the like, and can intuitively detect genotypes of different individuals of the Scylla paramamosain so as to quickly acquire a polymorphic map for hereditary variation at functional gene microsatellite loci of the Scylla paramamosain; and the microsatellite markers can be applied to the fields such as hereditary variation analysis of the Scylla paramamosain, germ plasm resource protection and management, genetic linkage map construction and the like.

Description

technical field [0001] The invention belongs to the field of detection of DNA molecular genetic markers of Scylla pseudomae, in particular to a rapid detection method for microsatellite markers of functional gene sources of Scylla pseudomae. Background technique [0002] Microsatellite DNA (Microsatellite DNA) exists in almost all eukaryotic genomes. It is a simple sequence repeat (Simple Sequence Repeats, SSR) composed of 1-6 nucleotides. It is a new type of molecule developed in the past ten years. Marking technology. Microsatellites have the advantages of large number, random distribution, high polymorphism, strong repeatability, co-dominant inheritance, and simple operation, and are widely used in population genetic diversity analysis, germplasm resource protection and management, and genetic linkage maps. Construction and QTL mapping and other fields. [0003] Scholars at home and abroad have developed microsatellite markers in most important aquaculture animals. For...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 马洪雨马凌波马春艳
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com