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Method for rapidly breeding sweet-waxy double-recessiveness maize selfing line

A technology of sweet waxy corn and sweet glutinous double, applied in the field of corn genetics and breeding, can solve the problems of reducing field workload and breeding land, shortening the breeding cycle, and cumbersome process, so as to reduce field workload, improve breeding efficiency, and use simple materials Easy to get effect

Inactive Publication Date: 2017-03-22
湖南省作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using two generations of test crosses to identify sweet and waxy double recessive genotypes, the cycle is long, the process is complicated, and the efficiency is low; while the markers linked to the wx gene are used to identify sweet materials, although sweet materials can be identified in the sweet S1 family. The plants with the glutinous double recessive gene can eliminate the plants that do not contain the sweet and waxy double recessive gene in the early generation, shorten the breeding cycle, reduce the field workload and breeding land, but the process is cumbersome and expensive
In addition, it is necessary to have good sweet and waxy corn inbred lines in hand to carry out the breeding of sweet and waxy double recessive inbred lines. The threshold is relatively high, and the time required for sweet and waxy corn inbred lines , it takes a long time to breed a sweet and waxy double recessive inbred line

Method used

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  • Method for rapidly breeding sweet-waxy double-recessiveness maize selfing line
  • Method for rapidly breeding sweet-waxy double-recessiveness maize selfing line
  • Method for rapidly breeding sweet-waxy double-recessiveness maize selfing line

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Experimental program
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Effect test

Embodiment 1

[0044] Xixing colorful sweet waxy corn is a sweet waxy corn hybrid with the genotype Sh2sh2wxwx obtained by crossing the double recessive homozygous inbred line sh2sh2wxwx with the waxy corn inbred line Sh2Sh2wxwx, which is used as the sweet waxy double recessive corn self-bred of the present invention. The parent of interbreeding, its method comprises the steps (as figure 1 shown):

[0045] (1) In the spring of 2013, the Xixing colorful sweet waxy corn variety will be self-pollinated;

[0046] (2) On the ears obtained after step (1), select the seeds whose performance character is sweet quality, that is, the phenotype is sunken and shrunken (the genotypes are su1su1wxwx, sh2sh2wxwx, bt2bt2wxwx), and planted in the autumn of 2013 to obtain F1 generation plants , from which a single plant that grows vigorously, has a moderate ear insertion position, and meets the requirements for plant type, disease resistance and growth period is selected for self-bagging;

[0047] (3) In th...

Embodiment 2

[0056] Buy the sweet waxy corn variety that genotype is respectively Su1su1wxwx, Sh2sh2wxwx and Bt2bt2wxwx on the market, use it as the parent of sweet waxy double recessive corn inbred line of breeding of the present invention, its method comprises the following steps:

[0057] (1) Testcross different varieties of sweet waxy corn with test species with different sweetness genes (su1su1, sh2sh2 or bt2bt2), and if the obtained progeny seeds show depression and shrinkage, it means that the sweetness gene of sweet waxy corn is compatible with the test The sweetness gene of the two species is the same, so as to identify the sweetness gene of sweet waxy corn, the progeny seeds obtained by the test cross are planted according to the difference of the sweetness gene, and then the interspecific loose powder is carried out among the varieties with the same sweetness gene;

[0058] (2) After step (1), on the fruit ears obtained by interspecific loose powder, continue to select seeds whos...

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Abstract

The invention discloses a method for rapidly breeding a sweet-waxy double-recessiveness maize selfing line. Self pollination or intraspecific free powder scattering is conducted on existing sweet-waxy maize varieties, seeds with the phenotype being sunken shrinking are selected from clusters obtained through selfing to be planted, and the stable sweet-waxy double-recessiveness maize selfing line is obtained after continuous selfing. According to the method, the sweet-waxy selfing line does not need to be bred by oneself, adopted materials are simple and easy to obtain, operation is easy and convenient, cost is low, and the breeding term is short. The invention further discloses the method for rapidly breeding the sweet-waxy double-recessiveness maize selfing line. Different sweet-waxy maize varieties are respectively planted according to different sweet genes, the interspecific crossing free powder scattering is conducted on varieties the same in sweet gene, the seeds with the phenotype being sunken shrinking are selected, and the stable sweet-waxy double-recessiveness maize selfing line is obtained after continuous selfing. According to the method, gene polymerization is facilitated, the excellent sweet-waxy double-recessiveness selfing line is easier to select, and a good foundation is laid for widening the maize germplasm resources.

Description

technical field [0001] The invention belongs to the field of corn genetics and breeding, in particular to a method for rapidly breeding sweet and waxy double recessive corn inbred lines. Background technique [0002] With the continuous development of the economy and the improvement of living standards, urban and rural residents have an increasing demand for fresh corn, and the quality requirements are also getting higher and higher. The current single sweet corn and waxy corn cannot meet the taste and flavor. the needs of the people. In recent years, my country's corn breeders have bred a batch of new fresh corn varieties with both sweet and waxy flavors—sweet waxy corn, which is more and more popular among consumers because of its sweetness and waxy taste. Breeding sweet and waxy maize with sweet and waxy double recessive genes has become the research direction of fresh corn breeding. [0003] The requirement for the selection of sweet-waxy corn varieties is that one par...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 曹钟洋陈志辉郭欢乐陈松林李涵
Owner 湖南省作物研究所
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