Method for improving self-pollination seed setting percentage of diploid potatoes and application of method

A potato, diploid technology, applied in the directions of application, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of long hybrid breeding cycle, low expression frequency of recessive genes, heterozygosity, etc. effect of fertility

Inactive Publication Date: 2015-11-18
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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Problems solved by technology

The genome of tetraploid potato is highly heterozygous, and the expression frequency of recessive genes is low, so that many harmful alleles are hidden in tetraploids, and beneficial alleles are difficult to recombine into a tetraploid variety, which is An important reason for the long cycle of potato hybrid breeding
However, diploid potatoes are mostly self-incompatible, so the problem of self-incompatibility needs to be overcome first

Method used

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  • Method for improving self-pollination seed setting percentage of diploid potatoes and application of method
  • Method for improving self-pollination seed setting percentage of diploid potatoes and application of method
  • Method for improving self-pollination seed setting percentage of diploid potatoes and application of method

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Experimental program
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Effect test

Embodiment 1

[0034] 1) Diploid potato materials were planted in Beijing. During the flowering period, flower buds at different times from flowering were selected for grading. For details, see figure 1 , Ji Leiling are respectively:

[0035] 0: On the day of flowering, the petals are fully open;

[0036] -1: 1 day before flowering, the petals have just opened;

[0037] -2: 2 days before flowering, the petals exceed 1 / 3 of the calyx;

[0038] -3: 3 days before flowering, the petals are flush with the calyx;

[0039] -4: 4 days or more before flowering: Petals are enclosed by calyx.

[0040] 2) Sampling of flower buds of different bud ages to extract total RNA and total protein.

[0041] qRT-PCR method was used to detect the mRNA expression of S-RNase gene, and the detection results are shown in figure 2 , indicating that the expression of S-RNase increased gradually before flowering.

[0042] Westernblotting method was used to detect the protein expression of S-RNase gene, the test re...

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Abstract

The invention relates to the field of plant breeding and specifically discloses a method for improving the self-pollination seed setting percentage of diploid potatoes. The method comprises: carrying out artificial self-pollination on diploid potato flower buds, preferably carrying out artificial self-pollination on the flower buds with a relatively low S-RNase gene expressive quantity. Self-incompatibility of the diploid potatoes is overcome by selecting a proper pollination time for self-pollination. The method is simple and feasible. No exogenous wild genomes are needed to be transferred while self-incompatibility is overcome, so that the obtained self-pollinated seeds can be directly applied to a process of genetic research and breeding.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a method for improving the self-seed setting rate of diploid potatoes and its application. Background technique [0002] The slow progress of potato breeding is mainly determined by the potato tetraploid genetic characteristics. The genome of tetraploid potato is highly heterozygous, and the expression frequency of recessive genes is low, so that many harmful alleles are hidden in tetraploids, and beneficial alleles are difficult to recombine into a tetraploid variety, which is An important reason for the long cycle of potato hybrid breeding. Compared with tetraploid potato, diploid potato genome is relatively simple, and harmful genes can be eliminated through continuous selfing, so as to obtain excellent inbred lines for hybrid production. Diploid potato hybrid breeding can achieve directional improvement of target traits, speed up the potato breeding process, and is also conduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 黄三文张春芝伦尧尧
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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