Process for overcoming self-incompatibility of plant by laser

A process method and affinity technology, applied in the field of laser technology to overcome plant self-incompatibility, to achieve the effects of strong operability, increased biological activity, and increased seed setting rate

Inactive Publication Date: 2003-11-19
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of science and technology, laser technology, as the most promising technical means, is being widely used in the medical field. Trial experiments have just begun in agriculture, but there is no report on the application of laser in overcoming self-incompatibility.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Collect mature grassland gentian anthers and place them in a ventilated and dry condition for 2-3 hours. When the pollen begins to shed, the pollen is treated with He-Ne laser and pollinated on the mature stigma. The laser power is 9mw, the diameter of the light spot is 1.5mm, and the treatment time is 2min to irradiate, and the seed setting rate has increased by 49%.

Embodiment 2

[0039] Collect mature kale anthers, and place them under ventilated and dry conditions for 1.5-2 hours. When the pollen begins to shed, the pollen is treated with He-Ne laser, and the pollen is pollinated on the mature stigma. The laser power is 9mW, the spot diameter is 1.5mm, and the treatment time is 8min. The self-compatibility index is increased by 4.4 times.

Embodiment 3

[0041] Collect the anthers of mature strawberries, and place them under ventilated and dry conditions for 1-1.5 hours. When the pollen starts to shed, the pollen is treated with carbon dioxide laser, and the pollen is pollinated on the mature stigma. The laser power is selected to be 100mW-50W, the spot diameter is 0.5mm-50mm, and the treatment time is 1-30sec for irradiation.

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PUM

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Abstract

A technology for overcoming the self-incompatibility of plant by laser includes such steps as picking up the mature anthers, drying for 1-3 hr until pollen is released, treating the pollen by laser, and pollinating within 1-5 hr. It can increase the setting percentage by 20-40%.

Description

Technical field [0001] The method of the invention relates to a process for overcoming plant self-incompatibility by using laser light. Background technique [0002] Self-incompatibility is a common phenomenon in the plant world, and it is a reproductive isolation that is beneficial to cross-pollination formed during the long-term evolution of plants. Self-incompatibility of plants refers to the phenomenon that hermaphroditic plants that can produce male and female gametes with normal functions and mature at the same time cannot complete fertilization during self-pollination or cross-pollination of the same genotype. Self-incompatibility has important theoretical and practical significance in plant reproductive biology and heterosis utilization. [0003] The breeding and utilization of self-incompatible lines is an important discovery in the utilization of heterosis, which can save the labor of manual emasculation, reduce the cost of seed production, improve the efficiency ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 李玉花张旸杨杰阎海芳周波李昀辉赵飞许志茹
Owner NORTHEAST FORESTRY UNIVERSITY
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