Novel method for transplanting winter wheat young embryo one-step seedlings big summer fields for fast growing
A technology of winter wheat and a new method, applied in the field of crop breeding, can solve the problems of unfavorable popularization and application, high cost of scientific research, long time to mature, etc., and achieve the effects of facilitating popularization and application, reducing scientific research investment, and less scientific research investment
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Embodiment 1
[0019] Example 1 First-year winter wheat immature embryo one-step seedling transplantation in summer field rapid growth experiment
[0020] 1. Planting of parent material
[0021] On October 6, 2008, the mature seeds of winter wheat Jimai 20 and Yanyou 361 were sown in the agronomy experimental field of Shandong Agricultural University.
[0022] 2. Get contemporary hybrid F 1 immature embryo
[0023] Using the conventional sexual hybridization method, Jimai 20 was emasculated for 3-5 days, and on May 1, 2009, the mature pollen of Yanyou 361 was smeared on the stigma of the female parent Shannong 15 to obtain the contemporary hybrid F 1 Immature embryos, namely Jimai 20×Yanyou 361→F 1 Immature embryos were placed, and the date of pollination was marked on the hybrid ears.
[0024] 3. Young embryos grow into seedlings in one step: on May 15, 2009, the above-mentioned contemporary hybrid F 1 Immature embryos on day 14 were sterilized with 75% ethanol for 8 minutes, rinsed wi...
Embodiment 2
[0031] Example 2 One-step seedling transplantation of winter wheat immature embryos in the second year to field field rapid growth test in summer
[0032] 1. Planting of parent material
[0033] On October 7, 2009, the winter wheats Tainong 18 and Zhongyou 9507 were sown in the agronomy experimental field of Shandong Agricultural University.
[0034] 2. Get contemporary hybrid F 1 immature embryo
[0035] On May 2, 2010, using conventional sexual crossing method, obtained Tainong 18×Zhongyou 9507→F 1 contemporary hybrid immature embryos,
[0036] Thereafter from F 1 Embryo inoculation, vernalization of embryo seedlings, site fertilization, hardening, transplanting of embryo seedlings, field management of embryo seedlings, etc. are the same as the one-step seedling transplantation of first-year winter wheat embryos in the summer field in Example 1. The difference is that The embryonic age of inoculated immature embryos was 16 days, and the vernalization time was 20 days. ...
Embodiment 3
[0037] Example 3 One-step seedling transplantation of winter wheat immature embryos in the third year to field field rapid growth test in summer
[0038] 1. On October 5, 2010, the winter wheat Jimai 20 and Nongda Youfan 5 were sown in the agronomy experimental field of Shandong Agricultural University.
[0039] 2. Get contemporary hybrid F 1 immature embryo
[0040] On May 5, 2011, Jimai 20 × Nongda Youfan 5 → F was obtained by conventional sexual hybridization 1 contemporary hybrid immature embryos,
[0041] Thereafter from F 1 The method from inoculation to transplanting was the same as the one-step seedling transplantation of winter wheat immature embryos in the previous two years in the summer field plus generation experiment, the difference was that the immature embryos were inoculated for 14 days and vernalized for 20 days.
[0042] The focus of the present invention is to first inoculate the 14-16d immature embryos of winter wheat on the MB medium to obtain one-step ...
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