Male-seducing seed-reserving method during middle and later period of cucumber female plant growth
A technology for female lines and cucumbers, which is applied in the field of male seduction and seed retention, can solve problems such as difficulty in pollination and fertilization, and achieve the effect of overcoming time constraints.
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Embodiment 1
[0015] F 2 group. All-female offspring without collaterals were selected from this population. Spraying silver nitrate to induce males in the early stage of plant growth will interfere with the selection of all-female traits, and the traits of no side branches can not be fully and clearly judged until the middle and late stages of plant growth (after the plant grows to 20 nodes), so it is necessary to use it in the middle and late stages of plant growth. Make selections.
[0016] random planting F 2 There were 100 plants in the 6106 population, and 10 plants with all females and no side branches were selected when the plants grew to 20 nodes. Each plant selects 2-3 leaf axils with flower buds less than 0.5mm on the main vine, sprays 300 mg / L silver nitrate on the leaf axils, and repeats after 2 days; Two days later, the male flowers appear; select female flowers of the same period on other nodes, and carry out flower clamping treatment on the female and male flowers respec...
Embodiment 2
[0018] Cucumber all-female line S48.
[0019] When the plant grows to 16 nodes, each plant selects 2 leaf axil positions that have flower buds less than 0.5mm on the main vine, sprays 300mg / L silver nitrate on the leaf axil positions, and repeats once every 2 days; Male flowers appeared 7 days after silver nitrate was applied; female flowers of the same period were selected at other nodes, and the female and male flowers were respectively interpolated the day before flowering; Carry out artificial pollination and keep the seeds, and still clamp the female flowers after pollination (to prevent pollination). Thus, a single selfed seed of the all-female line S48 was obtained.
[0020] Compared with spraying silver nitrate on the terminal buds to induce males, the present invention overcomes the limitation that artificial male induction can only be carried out in the early stage of plant growth, and also has the advantage of only targeting a single node to induce males, and impro...
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