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Molecular marker Indel6 for oil related gene and application of molecular marker

A marker and gene technology, applied in the direction of DNA / RNA fragments, microbial measurement / inspection, recombinant DNA technology, etc., can solve the problem of low accuracy, achieve the effect of heavy workload, long cycle, and broaden the genetic background

Active Publication Date: 2019-05-31
HENAN AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0004] In recent years, with the development of molecular marker technology, many researchers have used markers linked to oil-related QTLs to perform assisted selection on soybean germplasm. This method improves the speed of selection to a certain extent and is easy to operate, but These markers are linkage markers of soybean oil gene, so the accuracy is generally low

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  • Molecular marker Indel6 for oil related gene and application of molecular marker
  • Molecular marker Indel6 for oil related gene and application of molecular marker

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Embodiment 1

[0025] (1) Test materials

[0026] Using 15 soybean core germplasms with different oil content provided by the National Soybean Improvement Center, including 6 high-oil germplasms, 7 low-oil germplasms and 2 medium oil content germplasms, as shown in Table 1:

[0027] Table 1. 15 soybean germplasms with different oil content

[0028] Numbering

Material

Oil content (%)

Oil content

1

Nannong 1138-2

17.52

Low / L

2

Zhou Bean 29

17.25

Low / L

3

Soviet Union 1

17.18

Low / L

4

Mid Bean 14

16.91

Low / L

5

Nannong 94-156

17.83

Low / L

6

Yudou 16

16.79

Low / L

7

wave height

18.52

Medium / M

8

spring beans

18.04

Medium / M

9

Shangqiu 73

17.19

Low / L

10

Kefeng 1

20.25

High / H

11

Zhou Dou 94010

19.95

High / H

12

Jianli Beef Hair Yellow

21.96

High / H

13

...

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Abstract

The invention provides a functional molecular marker Indel6 for oil related characters and application of the molecular marker, and relates to the technical field of molecular markers. In the functional molecular marker Indel6 for the oil related characteristics, the oil related gene GmOLEO1 is located at the 20th chromosome of soybean, and the CDS sequence of the oil related gene GmOLEO1 is shownin the SEQ ID NO.1. The marker Indel6 can be used for parting and identifying a new oil related material, and used for verifying soybean germplasms which are obtained through a common chemical methodin the earlier stage and are different in oil content, and the accuracy reaches 85% or above. Compared with a common screening method, time and labor are saved, the cost is reduced, the method is very high in reliability, and a new material and technology are provided for further oil related gene type germplasm verification and assistant selection of the molecular marker for soybean variety breeding.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to an oil-related gene molecular marker Indel6 and its application. Background technique [0002] Soybean is an important food crop and oil crop in my country. But the current dependence on foreign countries has reached over 85%. Therefore, exploring the genetic potential of soybean itself and cultivating new high-oil soybean varieties through molecular breeding and other technical means is an effective way to solve the above problems. [0003] Although a lot of progress has been made in soybean oil-related research, there are still some difficulties in the screening of different oil genotypes, mainly because the oil content must be measured with soybean grain samples, which are seasonal and oil content The content will undergo changes such as degradation when the grain sample is not stored properly, so the determination of large groups is not only time-consu...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 张丹董中东刘伟马兴立李忠锋
Owner HENAN AGRICULTURAL UNIVERSITY
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