The present invention provides a process for preparing cross-linked
sodium hyaluronate microspheres capable of being adopted as an emboliaztion agent by adopting
sodium hyaluronate as a
raw material. The process comprises the following steps: preparing a
sodium hyaluronate alkaline solution gel with a concentration of 10-30% g / ml; adding the
sodium hyaluronate alkaline solution gel to an emulsifier-containing
oil phase, and carrying out high speed emulsification through a shearing
machine, wherein an emulsification speed is 500-2000 rpm, and a time is 10-20 min; adding a certain amount of a cross-linking agent, stirring for 4-6 h at a
room temperature, carrying out a cross-linking reaction, and standing overnight after completing the reaction, wherein a
mass percentage of the cross-linking agent in the
oil phase is 0.2-2%; and adopting a water-soluble
organic solvent to wash to remove the
oil phase remained on the surface of the microspheres, and finally
drying to obtain the cross-linked
sodium hyaluronate microspheres. According to the present invention, the preparation process is simple; and the size of the obtained microspheres is suitable for routine
blood vessel emboliaztion, and the obtained microspheres have characteristics of controllable particle size, good
microsphere shape, easy screening, elasticity, expandibility, no toxic-
side effect on
human body, good
biocompatibility, good biodegradability, and ensured
clinical safety.