Pre-eclampsia disease animal model and construction method and application thereof

A preeclampsia, animal model technology, applied in sexual diseases, drug combinations, pharmaceutical formulations, etc., can solve the problems of tedious modeling process, incomplete recovery, and high modeling cost of model animals.

Active Publication Date: 2022-05-10
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at a series of technical problems of the above-mentioned model animals, such as high modeling cost, cumbersome modeling process, and pathophysiological processes that cannot be fully restored, the present invention provides a A new animal model, Sirt1+ / - whole body knockout heterozygote state can restore the pathophysiological process of the disease, the release of pregnancy state or treatment with agonists can restore

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  • Pre-eclampsia disease animal model and construction method and application thereof
  • Pre-eclampsia disease animal model and construction method and application thereof
  • Pre-eclampsia disease animal model and construction method and application thereof

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Embodiment Construction

[0047] The present invention will be further explained below in conjunction with the embodiments and the accompanying drawings. The following examples are only used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0048] 1 Animal model of preeclampsia: Sirt1 + / - Construction of whole body knockout heterozygous pregnant mice

[0049] Due to the needs of follow-up experiments, the present invention uses a promoter-driven Cre-loxP system. Sirt1 introduced from JAX (The JacksonLaboratory) flox / flox Gene-edited mice (B6.129-Sirt1 tm3Fwa / DsinJ). And ordered Dppa3-IRES-Cre mice from Southern Model Organisms (Shanghai Southern Model Biotechnology Co., Ltd.), because Dppa3-Cre can effectively exert Cre recombinase activity in the early embryo and germ line Model effective commercial tool mouse. Entrusted Nanmodel Organisms to carry out hybridization, and after de-Cre breeding, Sirt1 was obtained + / - Whole body knockout of ...

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Abstract

The invention discloses a preeclampsia disease animal model. A Sirt1 + / -whole body knockout heterozygote pregnant mouse is used as the preeclampsia disease animal model. The invention also discloses a construction method and application of the gene. The preeclampsia disease animal model does not need external medicine and operative intervention, breeding does not need special feed and special feeding environment, but preeclampsia disease occurrence can be relatively completely reduced, and the preeclampsia disease animal model can be used for helping to clarify the morbidity process and mechanism of preeclampsia disease, and has a good application prospect. The method can also be used for assisting in screening and efficacy evaluation of drugs for preventing and treating preeclampsia diseases.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to an animal model of preeclampsia and its construction method and application. Background technique [0002] Preeclampsia (PE) is a multisystem progressive disease unique to pregnancy, mainly manifested as new-onset hypertension and proteinuria in the second half of pregnancy or postpartum; or new-onset hypertension and significant end-organ dysfunction, with or No proteinuria. It often leads to adverse pregnancy outcomes of mother and child and increases the risk of long-term cardiovascular disease. The etiology is unknown and there is no effective treatment. However, because clinical symptoms resolve after delivery, the latest guidelines attribute it to placental and maternal vascular dysfunction. [0003] Ideally, an animal model should exhibit the clinical features of the disease. PE is idiopathic in the second and third trimesters of pregnancy, manifested ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A61K48/00A61K49/00A61P15/00
CPCA01K67/0276A61K49/0008A61K48/005A61P15/00A01K2217/15A01K2227/105A01K2267/03A01K2267/0393
Inventor 顾蔚蓉王诚洁裴江南刘真真李明清
Owner THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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