Saccharopolyspora pogona delta Clu13-MmsA and construction method and application thereof

A technology of Saccharopolyspora spp. and its construction method, which is applied in the field of S. spp. ΔClu13-MmsA and its construction, and can solve the problems of unsatisfactory production of butenyl pleosyns, etc.

Active Publication Date: 2021-11-12
HUNAN NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Subsequently, in order to construct high-yielding spinosyn engineering strains, researchers conducted extensive research, including the two-component system SenX3-RegX3 (see "Rang J, He H, ChenJ, et al. SenX3-RegX3, an Important Two-Component System, Regulates Strain Growth and Butenyl-spinosyn Biosynthesis in Saccharopolyspora pogona.iScience.2020; 23(8):101398"), TetR family regulator (see "He H, Yuan S, Hu J, et al. Effect of theTetR family transcriptional regulator Sp1418 on the global metabolic network of Saccharopolyspora pogona. Microb Cell Fact.2020; 19(1):27"), GDP-fucose synthas...

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  • Saccharopolyspora pogona delta Clu13-MmsA and construction method and application thereof
  • Saccharopolyspora pogona delta Clu13-MmsA and construction method and application thereof
  • Saccharopolyspora pogona delta Clu13-MmsA and construction method and application thereof

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[0039] The present invention will be further described below in conjunction with the embodiments and accompanying drawings.

[0040] The chemical reagents used in the examples of the present invention were obtained through conventional commercial channels unless otherwise specified.

[0041] (1) Medium formula and culture conditions

[0042] (1) S. pogona seed activation medium CSM (Complete synthetic medium): TrypticSoyBroth 4.5g / L, Glucose 0.9g / L, Yeast extract 0.3g / L, MgSO 4 ·7H 2 O 0.22g / L; Inoculate monoclonal or 2% strain preservation solution into a 150mL shake flask equipped with 20mL of CSM, cultivate at 30°C and 220r / min for 48h;

[0043] (2) S. pogona synthetic fermentation medium SFM (Synthetic fermentation medium): Glucose 20.0g / L, Yeast extract 4.0g / L; inoculate 2% strain activation solution into a 300mL shake flask containing 50mL SFM, 30 Cultivate at 220r / min for 10d;

[0044] (3) R6 medium is used for conjugative transfer: sucrose 200g / L, dextrin 10g / L, ca...

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Abstract

The invention provides Saccharopolyspora pogona delta Clu13-MmsA and a construction method and application thereof. The Saccharopolyspora pogona delta Clu13-MmsA is preserved in the China Center for Type Culture Collection, and the preservation number of the strain is CCTCC NO: M2021825. The construction method of the Saccharopolyspora pogona delta Clu13-MmsA is easy and convenient to operate, the Saccharopolyspora pogona delta Clu13-MmsA is used for producing butenyl spinosad through fermentation, the yield of the butenyl spinosad is increased by 10.1 times compared with that of Saccharopolyspora pogona original bacteria, and meanwhile the yield of the butenyl spinosad is far higher than that of an S.pogona-MmsA strain.

Description

technical field [0001] The present invention relates to a strain of Saccharopolyspora mentabilis and its construction method and application, in particular to a strain of Saccharopolyspora mentasyn ΔClu13-MmsA with high production of butenyl spinosad and its construction method and application. Background technique [0002] Crop pests are one of the main obstacles to the sustainable development of modern agriculture in my country. Traditional chemical pesticides can resist the invasion of pests, but they are easy to remain, which will pollute and damage the environment and cause food safety problems. Biopesticides are gradually being valued because of their strong selectivity, non-toxic and harmless, and not easy to produce resistance. With the improvement of people's awareness of ecological environment and food safety, the demand for green biopesticides for environmental and crop safety is also increasing. [0003] Butenyl-spinosyn (butenyl-spinosyn), which is produced by...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/74C12P19/62C12R1/01
CPCC12N9/0008C12N15/74C12Y102/01015C12P19/62
Inventor 夏立秋何昊城丁学知穰杰
Owner HUNAN NORMAL UNIVERSITY
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