Therapeutic effect of forsythin on rats with sepsis

Abstract

The invention relates to the field of treatment of sepsis, specifically to a medicine capable of reducing mortality and relatively prolonging survival time. It is found through current researches that a forsythia extract has effects of destroying bacterial endotoxin and inhibiting various positive and negative bacteria. But the mechanism of action hasn't been completely illustrated clearly, thus affecting the promotion of the forsythia extract to the international market. By silica gel column chromatography, Saphadex LH-20 column chromatography, recrystallization and the like, the substance is separated from 50% forsythia ethanol extract. By chemical identification, H NMR spectroscopy, C NMR spectroscopy, thin-layer chromatography, high performance liquid chromatography and the like, structural identification is carried out on a substance, and its structure and name are determined. By replication of cecal ligation perforation animal model, the pathological process of human sepsis is simulated. Tail vein injection is carried out for drug delivery, and postoperative survival rate of rats is observed after operation. According to the invention, forsythin is used as a main component to prepare an injection which is used for treatment of sepsis.

Description

technical field [0001] The invention relates to the field of treating sepsis, in particular to a drug capable of reducing the death rate of rats and relatively prolonging the survival time. Background technique [0002] Sepsis refers to the presence of various pathogenic microorganisms or their toxins in the blood or tissues. It is a systemic inflammatory response (SIRS) of the body to infection. It is a severe infection, severe trauma, major surgery, severe Common complications such as acute pancreatitis and shock, further development can lead to septic shock, acute respiratory distress syndrome (ARDS) and multiple organ dysfunction syndrome (MODS). Difficulty in treatment has become a worldwide problem. Foreign epidemiological surveys show that the fatality rate of sepsis has surpassed that of acute myocardial infarction. More than 350,000 people die from this disease in Europe and the United States every year, and there are more than 18 million cases of severe sepsis in ...

AI Technical Summary

Problems solved by technology

At present, a large number of clinical observations and preliminary animal experiments have found that the traditional Chinese medicine Forsythia has a very good curative effect on the prevention and treatment of sepsis. It has inhibitory effect on various Gram-positive and negative bacteria

At the same time, in vitro experiments found that forsythia extract has a strong effect of destroying bacterial endotoxin, but because of the complex composition of traditional Chinese medicine, the mechanism of action has not been fully elucidated, which has affected its promotion to the international market

Images