Therapeutic effect of forsythin on rats with sepsis

A technology of forsythin and sepsis, applied in the field of mortality, can solve the problems of complex ingredients of traditional Chinese medicine, influence on popularization, and incomplete explanation of the mechanism of action, and achieve the effect of reducing mortality

Inactive Publication Date: 2016-08-24
李志军
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a large number of clinical observations and preliminary animal experiments have found that the traditional Chinese medicine Forsythia has a very good curative effect on the prevention and treatment of sepsis. It has inhibitory effect on various Gram-positive and negative bacteria
At th...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Therapeutic effect of forsythin on rats with sepsis
  • Therapeutic effect of forsythin on rats with sepsis
  • Therapeutic effect of forsythin on rats with sepsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] Preparation of sepsis rat model

[0025]The rat cecum ligation and perforation (CLP) model was adopted, fasting for 12 hours before the operation, water incessantly, 5% chloral hydrate anesthesia (350-400mg / kg) intraperitoneal anesthesia, skin preparation in the middle of the anterior abdomen, disinfection of the abdominal skin, and doing in the middle of the abdomen 2.5cm incision, after taking out the cecum, free the mesentery and cecum, about 1 / 3 of the total length of the cecum from the junction of the cecum and ileum, try to avoid vascular ligation with No. Blood vessels, three-edged needle (2.6×65mm) pierced through the hole, 3×30mm adhesive strips penetrated through the cecum to stay, put it back into the abdominal cavity, and sutured the incision. After the operation, 10 mL of subcutaneous fluid was given to the neck to replenish the fluid loss during the operation and to resist shock. Rats in the sham-operated group were anesthetized and laparotomed, only the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of treatment of sepsis, specifically to a medicine capable of reducing mortality and relatively prolonging survival time. It is found through current researches that a forsythia extract has effects of destroying bacterial endotoxin and inhibiting various positive and negative bacteria. But the mechanism of action hasn't been completely illustrated clearly, thus affecting the promotion of the forsythia extract to the international market. By silica gel column chromatography, Saphadex LH-20 column chromatography, recrystallization and the like, the substance is separated from 50% forsythia ethanol extract. By chemical identification, H NMR spectroscopy, C NMR spectroscopy, thin-layer chromatography, high performance liquid chromatography and the like, structural identification is carried out on a substance, and its structure and name are determined. By replication of cecal ligation perforation animal model, the pathological process of human sepsis is simulated. Tail vein injection is carried out for drug delivery, and postoperative survival rate of rats is observed after operation. According to the invention, forsythin is used as a main component to prepare an injection which is used for treatment of sepsis.

Description

technical field [0001] The invention relates to the field of treating sepsis, in particular to a drug capable of reducing the death rate of rats and relatively prolonging the survival time. Background technique [0002] Sepsis refers to the presence of various pathogenic microorganisms or their toxins in the blood or tissues. It is a systemic inflammatory response (SIRS) of the body to infection. It is a severe infection, severe trauma, major surgery, severe Common complications such as acute pancreatitis and shock, further development can lead to septic shock, acute respiratory distress syndrome (ARDS) and multiple organ dysfunction syndrome (MODS). Difficulty in treatment has become a worldwide problem. Foreign epidemiological surveys show that the fatality rate of sepsis has surpassed that of acute myocardial infarction. More than 350,000 people die from this disease in Europe and the United States every year, and there are more than 18 million cases of severe sepsis in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/7048A61P31/04
Inventor 李志军
Owner 李志军
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap