Construction method and application of gout animal model

A technology of animal model and construction method, which is applied in the field of animal models, and can solve problems such as no occurrence of gout

Active Publication Date: 2019-10-18
ANHUI PROVINCIAL HOSPITAL
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Problems solved by technology

In animal models with sustained high levels of blood uric acid obtained by knocking out the uricase gene, no reports of gout have been reported

Method used

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  • Construction method and application of gout animal model
  • Construction method and application of gout animal model
  • Construction method and application of gout animal model

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Experimental program
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Embodiment 1

[0035] This embodiment provides a method for constructing a gout animal model

[0036] 1. Establishment of gout rat model

[0037] 1. be divided into three groups by the male rat of body weight 180-220g, comprise normal group, Coderre method gout model group, the present invention model group; Every group 10.

[0038] 2. Inject the uricase inhibitor into the rat abdominal cavity according to the body weight of the rat, the injection dose is 30mg / 100g body weight, and the injection time is fixed, once a day for 7 days;

[0039] 3. After one week, use uric acid test paper to detect the uric acid content in the rat body, and carry out follow-up tests on rats with a uric acid value higher than 180 μmol / L;

[0040] 4. After confirming that the rats were in a state of hyperuricemia, on the first day, the tail vein injection concentration of hyperuricemia rats was 0.1g / ml of urate crystal suspension, and the dose was 50 μl / only;

[0041] 5. Detect the uric acid content in the rats th...

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Abstract

The invention discloses a construction method and application of a gout animal model. The construction method comprises the steps of injecting a uricase inhibitor into the abdominal cavity of a rat every day for one week to inhibit the activity of uricase of the rat and meanwhile injecting uric acid salt crystals and ATP into the tail vein to make the rat spontaneously develop gouty arthritis. Theprovided construction method of the gout animal model can stably and rapidly induce gout in the rat, and the clinical manifestation and pathological characteristics of the model are completely matched with the characteristics of human gout. According to the gout model constructed by means of the method, massive uric acid salt crystals can be observed under a polarizing microscope in a diseased joint, and infiltration of massive neutrophilic granulocytes can be found through immunohistochemistry. In addition, the invention also relates to the application of the gout animal model constructed bymeans of the method in research on the occurrence and remission mechanisms of gout and related inflammation, screening and evaluation of therapeutic drugs and the like.

Description

technical field [0001] The invention relates to the technical field of animal models, in particular to a construction method and application of a gout animal model. Background technique [0002] Gout is an inflammatory disease associated with hyperuricemia, mainly manifested as recurrent gouty arthritis, uric acid urinary calculi, and gouty nephropathy. With the change of lifestyle, the prevalence of gout and hyperuricemia in China has increased significantly. It is estimated that there are 180 million patients with hyperuricemia and more than 30 million patients with gouty arthritis. The onset of gout is extremely painful, seriously threatening people's healthy life. [0003] Research on gouty arthritis needs to be carried out with the help of animal models with gout-like performance. However, currently available gout models have many shortcomings. Coderre et al. invented the method of joint local injection and established a gout model. This method mainly injects monoso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A01K2207/20A01K2227/105A01K2267/03A61K49/0008
Inventor 陶金辉张宏亮朱子文唐玉洁李心亚
Owner ANHUI PROVINCIAL HOSPITAL
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