Melanin/Ce6 photodynamic nano drug with enhanced light absorption and preparation method of melanin/Ce6 photodynamic nano drug

A nano-drug, melanin technology, applied in the directions of non-active ingredients medical preparations, active ingredients-containing medical preparations, drug combinations, etc., to achieve the effect of good biocompatibility

Active Publication Date: 2018-05-08
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used carriers only have the simple function of transporting drugs

Method used

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  • Melanin/Ce6 photodynamic nano drug with enhanced light absorption and preparation method of melanin/Ce6 photodynamic nano drug
  • Melanin/Ce6 photodynamic nano drug with enhanced light absorption and preparation method of melanin/Ce6 photodynamic nano drug
  • Melanin/Ce6 photodynamic nano drug with enhanced light absorption and preparation method of melanin/Ce6 photodynamic nano drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation method of melanin powder is made by the following steps:

[0043] Take 10mg of washed and dried human hair, put it in a round bottom flask, add 100ml of 37% concentrated hydrochloric acid, heat and reflux at 110-112°C for 4h with a reflux device equipped with hydrogen chloride absorption, and dialyze with a dialysis membrane for 24h after cooling , to obtain crude melanin particles. The melanin particles are centrifuged at 12000rpm, and the brown supernatant is taken, which is the aqueous solution of 30-70nm melanin nanoparticles, and its transmission electron microscope photo is as follows: figure 1As shown, the measured hydrated particle size distribution is shown in figure 2 shown. The aqueous solution of melanin nanoparticles was freeze-dried for 24 hours to obtain loose melanin powder, ready for use.

Embodiment 2

[0045] A preparation method of melanin / Ce6 photodynamic nano-medicine with enhanced heat absorption, comprising the following steps:

[0046] Prepare Ce6 into a high-concentration mother solution of 20mg / ml DMSO, and add it dropwise to the melanin aqueous solution (0.5mg / ml) according to a certain ratio (the mass ratio of melanin: Ce6 is 1:1, 1:0.6 and 1:0.2, respectively). , mixed for 6 hours, and then ultrafiltered twice using a 10k ultrafiltration tube, the ultrafiltration speed was 5000rpm, and the ultrafiltration time was 10min to obtain melanin / Ce6 nanoparticles.

Embodiment 2-1

[0048] The difference from Example 2 is that the concentration of the Ce6 high-concentration DMSO mother solution is 5 mg / ml, and the concentration of the melanin aqueous solution is 0.01 mg / ml.

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Abstract

The invention relates to a melanin / Ce6 photodynamic nano drug with enhanced light absorption and a preparation method of the melanin / Ce6 photodynamic nano drug. The melanin / Ce6 photodynamic nano drugis prepared from melanin nano particles serving as a carrier and Ce6 molecules loaded on the melanin nano particles; the mass ratio of the melanin nano particles to the Ce6 molecules is 1 to (0.01 to10). Compared with the prior art, the synthesized melanin / Ce6 photodynamic nano drug with enhanced light absorption has the advantages that the shortcomings such as short retention time of Ce6 in body, little enrichment in the diseased region and strong skin phototoxicity can be avoided, and is expected to be enriched on the tumor site after tail vein injection, so as to perform specific photodynamic therapy on local tumor and the like.

Description

technical field [0001] The invention relates to the field of tumor photodynamic therapy, in particular to a melanin / Ce6 photodynamic nano-medicine with enhanced light absorption and a preparation method thereof. Background technique [0002] Ce6 (that is, chlorin e6) is a photosensitizer with excellent performance obtained by refining, refining and modifying chlorophyll. It has many advantages such as clear chemical structure, strong photodynamic reaction ability, low dark toxicity and fast metabolism in the body. It is the main component of a new generation of photodynamic cancer drugs in the research and development process. During photodynamic therapy, the photosensitizer Ce6 receives light irradiation at a specific wavelength, absorbs the energy of photons, undergoes a photochemical reaction, and generates reactive oxygen species (reactive oxygen species, ROS), which can effectively oxidize organic molecules in cells to achieve cell-killing effect. However, Ce6, as a p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/46A61P35/00
CPCA61K9/5176A61K41/0071
Inventor 刘萍任天斌李永勇董海青
Owner TONGJI UNIV
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