Model of outer marrow infiltration of leukemia-NOD/SCID mice and preparing method thereof

A leukemia and acute leukemia technology, which is applied in medical science, material inspection products, surgery, etc., can solve unclear problems and achieve the effect of long survival time and high success rate

Inactive Publication Date: 2009-10-07
天津中医药大学第一附属医院
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  • Abstract
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Problems solved by technology

However, there is no report on the establishment of human leukemia-NOD/SCID mouse extramedullary infiltration model by tail vein injection of bone marrow mononuclear cells from leukemia patients at home and abr

Method used

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  • Model of outer marrow infiltration of leukemia-NOD/SCID mice and preparing method thereof
  • Model of outer marrow infiltration of leukemia-NOD/SCID mice and preparing method thereof
  • Model of outer marrow infiltration of leukemia-NOD/SCID mice and preparing method thereof

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Embodiment Construction

[0044] The present invention will be further described in conjunction with the following examples, but the present invention is not limited to the following examples.

[0045] 1. Preparation of bone marrow mononuclear cells from leukemia patients

[0046] Select acute leukemia patients or chronic myelogenous leukemia blast patients, routine aseptic collection of bone marrow 5ml heparin anticoagulant. Using a 15ml centrifuge tube, first inject 5ml of lymphocyte separation solution, and then slowly inject bone marrow fluid to make it float on the upper layer of the separation solution; centrifuge horizontally at 1800r / min for 10min; move the centrifuge tube vertically and slowly into the ultra-clean workbench, and absorb White mononuclear cell suspension layer, washed twice with PBS, counted live cells with 2% trypan blue, and adjusted the final concentration of live cells to 5×10 7 / ml.

[0047] 2. Pretreatment of NOD / SCID mice

[0048] SPF grade NOD / SCID mice, 5 to 6 weeks ...

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Abstract

The present invention relates to a method for preparing model of outer marrow infiltration of leukemia-NOD/SCID mice, wherein the method comprises the following steps: 1, preparing a single karyocyte of marrow of leukemia patient, 2. preprocessing the NOD/SCID mice, 3. establishing a model, and 4. identifying the model. The invention firstly adopts a mode of injecting single karyocyte of marrow of leukemia patient through tail-vein injection for establishing the model of outer marrow infiltration of leukemia-NOD/SCID mice. Furthermore the prepared model of outer marrow infiltration of leukemia-NOD/SCID mice has the advantages of high success rate and long survival time. The invention can provide new animal model for researching the outer marrow infiltration mechanism of leukemia, sieving the leukemia medicine, and the targeted therapy of gene and molecule.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a human leukemia-NOD / SCID mouse extramedullary infiltration model and a preparation method thereof. Background technique [0002] With the changes of human living environment, living standards and lifestyles and the advancement of medicine, the spectrum of diseases has undergone significant changes, general infectious diseases have been gradually controlled, and malignant tumors have become increasingly common and seriously threaten human life and quality of life. one of the major diseases. Leukemia is a malignant tumor of the hematopoietic system and one of the most common malignant tumors in my country. According to the survey, the incidence of leukemia in each region occupies the sixth place among various tumors. Leukemia is the malignant proliferation of leukemia cells in bone marrow, spleen, liver and other hematopoietic organs, which can enter the blood circulation and infilt...

Claims

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Application Information

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IPC IPC(8): A61B19/00G01N33/48A61B90/00
Inventor 杨文华
Owner 天津中医药大学第一附属医院
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