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Construction method of type I diabetes animal model

An animal model and construction method technology, which can be applied in pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of blood sugar disorder in animals, prone to complications, severe diabetes in animals, etc., and achieve simple modeling. , The effect of high mold rate and short cycle

Inactive Publication Date: 2019-05-07
贵州健安德科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgical modeling has high requirements for operators and is prone to complications. The success rate of virus-induced modeling is not high, there are side effects, and the cycle of fattening and diet induction is long
The dose window of alloxan is very narrow, which can easily lead to blood sugar disturbance in animals, and then lead to severe diabetic symptoms and subsequent death of animals

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] laboratory apparatus

[0025] Blood glucose measuring instrument (Johnson & Johnson), precision electronic balance, pH meter, pipette, syringe, etc.

[0026] Experimental Animals and Materials

[0027] experimental animals

[0028] C57BL / 6 mice were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0029] Experimental Materials

[0030] Citric acid monohydrate, trisodium citrate dihydrate, streptozotocin (Biosharp, Sigma S0130), dextrose.

[0031] experimental method

[0032] Select 20 C57BL / 6 mice, randomly divide them into 2 groups, 10 mice in each group, and mark them separately; randomly select an appropriate amount of animals to measure fasting blood sugar, as the basal blood sugar level; inject streptozotocin solution 50 mg / kg·BW for 6 consecutive days , the control group was injected with the same amount of normal saline, and the tail vein was changed to intraperitoneal injection for the first time. Before each injection, they w...

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Abstract

The invention provides a construction method of a type I diabetes animal model. The method comprises the following steps: selecting C57BL / 6 mice, performing first tail vein injection, continuously performing intraperitoneal injection of a streptozotocin reagent for 5 days, performing ambrosia for 12 hours before injection, and allowing the mice to freely drink water. Two days after the injection procedure is completed, the ambrosia is carried out for 4 hours, and a glucometer is used for measuring blood glucose until the blood glucose value is 10 mmol / L or above. The statistical analysis of the blood glucose measurement result of the type I diabetes animal model shows that the C57BL / 6 mouse type I diabetes model constructed by using the streptozotocin is feasible and can be used for researching the diabetes pathogenesis and the anti-hyperglycemia intervention of medicaments and health-care foods. Compared with the prior art, the C57BL / 6 mouse type I diabetes model constructed by the invention has the advantages of high film forming rate, simple operation, no death of animals and good reproducibility.

Description

technical field [0001] The present invention relates to a method for constructing a type I diabetes animal model, in particular to a method for constructing a C57 mouse type I diabetes model. Background technique [0002] Diabetes has become one of the important diseases threatening human health and safety. Diabetes and its complications have brought a heavy burden to human health and social development. Diabetes research methods are diverse and cover multiple levels, such as: animal level, cell level and protein level. As an indispensable and important means and method for diabetes research, animal models have received more and more attention. Animal models of diabetes are helpful to further reveal the pathogenesis and intervention measures of diabetes, and test and screen various hypoglycemic drugs. Animal models can be divided into spontaneous and experimental. Compared with experimental models, spontaneous models are less popular and cost more, while experimental mode...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7008A61K31/7004
Inventor 梅承翰杨鸿波陈蓓蓓张龙生张朝颖周志万牛厚菊朱哲青龙去桃张飞姜文敏李景壮包娜
Owner 贵州健安德科技有限公司
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