Method used for identifying and detecting danhong injection in vivo metabolites

A technology of metabolite identification and Danhong injection, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of unclear material basis of ischemic encephalopathy

Inactive Publication Date: 2017-08-15
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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Problems solved by technology

In recent years, the clinical application of Danhong Injection in the treatment of chest obstruction and stroke caused by blood stasis blockage has achieved positive curative effect, but the material basis for its treatment of ischemic encephalopathy is still unclear

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  • Method used for identifying and detecting danhong injection in vivo metabolites
  • Method used for identifying and detecting danhong injection in vivo metabolites
  • Method used for identifying and detecting danhong injection in vivo metabolites

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Embodiment Construction

[0072] In order to make the object, technical solution and positive effect of the present invention clearer, the present invention is further described in detail through the following examples. The following descriptions of specific embodiments are only used to explain the present invention, and do not limit the present invention;

[0073] A method for identification and detection of metabolites in Danhong injection liquid:

[0074] 1. Instrument: Qtrap liquid mass spectrometer (mass spectrometer AB SCIEX Qtrap 4500 System, liquid phase Shimadzu UFLCXR);

[0075] 2. Sample preparation

[0076] Danhong injection was injected into SD rats through the tail vein, the injection volume was 12 mg / kg, and 0.3 mL of orbital blood was collected at each time point of 10 min, 30 min, 60 min, and 6 h, and the blood samples were placed in a heparinized centrifuge tube at 4000 r / Plasma was separated by centrifugation. Draw up 200 μL of plasma with a micro-sampler, add methanol: acetonitr...

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Abstract

The invention discloses a method used for identifying and detecting danhong injection in vivo metabolites. The method comprises following steps: danhong injection is used for tail vein injection of SD rats, blood samples are collected at different time points, centrifugation is carried out so as to obtain blood plasma, the blood plasma is subjected to treatment, a Qtrap liquid chromatograph / mass spectrometer is adopted for gradient elution separation of obtained samples, and combination of a plurality of scanning modes, including EMS-IDA-EPI, (p)MRM-IDA-EPI, and NL / Pre-IDA-EPI, with MetabolitePilot<TM> metabolite idenfifying software is adopted for analysis of obtained metabolites. It is found that the II phase metabolites of active components, including tanshinol, protocatechuic acid, protocatechualdehyde, and rosmarinic acid, in the danhong injection are mainly a series of methylated, sulfated, methylated-sulfated, and glucuronidated metabolites. The metabolites change along with the passage of drug administration time, the concentations of most of the metabolites reaches C<max> in 10 to 30min, and most the original drug and the metabolites are eliminated in 6h. The method is simple, is high in repeatability and accuracy, is reliable, and is capable of providing basis for clinical applications of danhong injection.

Description

technical field [0001] The invention belongs to the field of drug detection and analysis, in particular to a method for identifying and detecting metabolites in Danhong injection liquid. Background technique [0002] Danhong Injection (Guoyao Zhunzi Z20026866) is obtained from two traditional Chinese herbal medicines, Danshen (dried roots and rhizomes of Salvia miltiorrhiza Bunge. of Labiatae) and safflower (dried flowers of Carthamustinctorius L. of Compositae) through modern technology. A standardized water-soluble ingredient preparation extracted from traditional Chinese medicine. Salvia miltiorrhiza and safflower are two traditional Chinese medicines that are often used together to treat cardiovascular and cerebrovascular diseases. According to the traditional Chinese medicine theory, the nature of salvia miltiorrhiza is mainly cold, and the nature of safflower is mainly warm. One rises and one falls, and they have the effects of promoting blood circulation and removing...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8822
Inventor 万嘉洋周惠芬丁志山万海同
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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