Aceglutamide metabolite identification and detection method

Abstract

The invention discloses an aceglutamide metabolite identification and detection method. The method includes: (1), taking Guhong injection, performing body tail intravenous injection on a SD rat, collecting venous blood from 5min to 8h according to timing, and centrifugally separating plasma; (2), adding acetonitrile into the plasma, mixing well before centrifuging, and taking supernatant as a sample for detection, wherein volume ratio of the plasma to acetonitrile is 1:3-3.5; (), detecting: adopting a quadrupole rod-linear ion trap tandom mass spectrometer to perform gradient elution separation on the sample, and combining MIM-IDA and Q3MI-IDA scanning methods to complete identification and detection of aceglutamide metabolite at the same time during one needle of sample injection through LightSight- software. Three isomer peaks different in retention time can be found in each of an aceglutamide standard sample and a plasma sample, and second-order spectra of the samples are different. Gamma-aminobutyric acid is not found, it is supposed that due to difference in tissue distribution, the gamma-aminobutyric acid exists in a nervous system more likely. The method is high in sensitivity, reliable and quick and provides a basis for qualitative and quantitative analysis of similar medicine in the future.

Description

technical field [0001] The invention belongs to the field of drug detection and analysis, and in particular relates to a method for identifying and detecting acetylglutamine metabolites. Background technique [0002] Acetylglutamine has the functions of maintaining the ability of nerve stress and improving brain function. After passing through the blood-brain barrier, acetylglutamine is first decomposed into γ-aminobutyric acid (GABA) and glutamic acid in astrocytes. After GABA is transported and released from glial cells, it binds to neuronal GABA receptors, inhibits post-synaptic neuron excitation, and inhibits the release of excitatory amino acids (EAA), resists excitotoxicity, and protects nerves; GABA can increase glucose phosphorylation Enzyme activity can promote the metabolism of glucose; GABA can also promote the synthesis of acetylcholine, improve nerve cell metabolism, have the ability to maintain nerve stress, and improve brain function. Acetylglutamine also ha...

AI Technical Summary

Problems solved by technology

At the same time, it overcomes some shortcomings of traditional 3D ion traps, such as low mass cut-off phenomenon (1 / 3 effect), low collision efficiency and poor quantitative analysis performance, etc.

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