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HDAC5 as a peripheral marker in a rat model of progeny infected during pregnancy

A technology of rat models and markers, applied in biochemical equipment and methods, microbiological determination/inspection, animal husbandry, etc., can solve problems such as the lack of early model quality evaluation standards

Active Publication Date: 2022-01-11
河南省精神病医院
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Problems solved by technology

[0002] Epidemiological studies have shown that maternal infection during pregnancy is an environmental risk factor for many mental diseases, and offspring rats infected during maternal pregnancy exhibit abnormal behaviors in adulthood, which can well simulate the clinical manifestations of many mental diseases. good models, but lacks early model quality evaluation criteria

Method used

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  • HDAC5 as a peripheral marker in a rat model of progeny infected during pregnancy
  • HDAC5 as a peripheral marker in a rat model of progeny infected during pregnancy
  • HDAC5 as a peripheral marker in a rat model of progeny infected during pregnancy

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Embodiment

[0023] The present invention provides a test method for evaluating HDAC5 as an early peripheral marker of the quality of an offspring rat model infected during pregnancy, comprising the following steps:

[0024] Step 1: Pregnant mouse preparation: Select SPF 10-14 week-old SD breed mice, and start breeding after 2 weeks of adapting to the new environment. Every 3-5 male mice and 1 female mouse are placed in a segmented cage for the first three days Only olfactory contact is allowed without physical contact. After 3 days, they are placed together for mating. The next morning, the female rats are checked through the vaginal suppository to see if the female rats have successfully conceived, and those who are successfully conceived are included in the group;

[0025] Step 2: Infection during pregnancy: 10 mg / kg polyinosinic acid (PolyI:C) or an equal volume of normal saline was injected into the tail vein on the 9th day after pregnancy was determined. Three animals were randomly se...

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Abstract

The present invention provides a test method for evaluating HDAC5 as an early peripheral marker of the quality of the offspring rat model infected during pregnancy, by injecting Poly I:C into the tail vein on the 9th day of pregnancy to activate the immune response of pregnant mice to prepare the offspring rat model infected during pregnancy, Comprehensive behavioral and HDAC5 expression analysis, found that the abnormal expression of HDAC5 in model rats appeared earlier than its abnormal behavior, and the change of HDAC5 expression in peripheral blood was consistent with the central reaction, so HDAC5 can be used as a tool for evaluating the quality control of offspring rat models infected during pregnancy Early peripheral marker molecules. The invention has ingenious conception and scientific scheme, and is of great significance to human epidemiological research and drug research and development.

Description

technical field [0001] The invention relates to the technical field of research on early peripheral markers of the quality of offspring rat models infected during pregnancy, in particular to a test method for evaluating HDAC5 as an early peripheral marker of the quality of offspring rat models infected during pregnancy. Background technique [0002] Epidemiological studies have shown that maternal infection during pregnancy is an environmental risk factor for many mental diseases, and offspring rats infected during maternal pregnancy exhibit abnormal behaviors in adulthood, which can well simulate the clinical manifestations of many mental diseases. good models, but lacks criteria for assessing the quality of earlier models. [0003] Studies at the animal level have confirmed that broad-spectrum HDACs inhibitors can improve cognition and alleviate the pathological state of neurodegenerative diseases, showing that HDACs dysfunction plays a role in mental diseases, suggesting ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02C12Q1/6883
CPCA01K67/02C12Q1/6883C12Q2600/158A01K2227/105A01K2267/03
Inventor 李文强苏玺杨勇锋宋盟邵明龙陈腾飞吕路线
Owner 河南省精神病医院
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