Corn photoperiod gene specific molecular marker and application thereof

A molecular labeling and photoperiod technology, applied in biochemical equipment and methods, DNA/RNA fragments, microbe determination/inspection, etc., can solve problems such as unconfirmed relationship with photoperiod and reduce environmental interaction

Inactive Publication Date: 2013-09-18
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although association analysis based on maize nested association populations can better improve the resolution of mapping populations, reduce environmental interactions, better detect epistasis and more accurately predict maize photoperiod-related QTL loci, but these mainly The relationship between QTL candidate genes and photoperiod has not been confirmed

Method used

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  • Corn photoperiod gene specific molecular marker and application thereof
  • Corn photoperiod gene specific molecular marker and application thereof
  • Corn photoperiod gene specific molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Gene sequence:

[0048] Firstly, using the genomic DNA sequences of the ZmPIF3 genes of the corn varieties CML166 and Zheng 58 cloned by the present inventors, the nucleic acid sequences were compared by software Mega4.0.

[0049] 2. Genomic DNA extraction

[0050] Genomic DNA of maize inbred lines CML166 and Zheng 58, and genomic DNA of F2 populations crossed between CML166 and Zheng 58 were extracted by CTAB method.

[0051] 3. Design primers based on the differences in known related sequences:

[0052] Comparing the partial DNA sequences of CML166 and Zheng 58 of the ZmPIF3 gene, the DNA sequence of the ZmPIF3 gene of CML166 is compared with the sequence of Zheng 58, and one of them has a transversion: the base of CML166 is G, while the base of Zheng 58 is a. Use the characteristics of sequence base transversion to design primers:

[0053] (1) ZmPIF3-CML166: forward: 5'-ACGCTGAGGGATGGATTGCTTG-3'

[0054] Reverse: 5'-AATGCACTGACAAGATGGTGGCTAT...

Embodiment 2

[0069] 1. Gene sequence:

[0070] First, use the genomic DNA sequences of the ZmPRR7 genes of the maize varieties CML166 and Zheng 58 obtained by cloning in the present invention, and use the software Mega4.0 to compare the nucleic acid sequences to obtain a sequence alignment.

[0071] 2, with the second step of embodiment 1.

[0072] 3. Design primers based on the differences in known related sequences:

[0073] Comparing the partial DNA sequences of CML166 and Zheng 58 in the ZmPRR7 gene by the software Mega4.0, the DNA sequence of the ZmPRR7 gene in CML166 is compared with the sequence of Zheng 58, and one of them has a transversion: the base of CML166 is T, and the base of Zheng The base of 58 is C (attached figure 2 ). Use the characteristics of sequence base transversion to design primers:

[0074] (1) ZmPRR7-CML166: forward: 5'-TTGAGAACCTTGTTTCACTAAAGGAT-3'

[0075] Reverse: 5'-CCGTAAGTGCTGCTATGAAGGTAT-3

[0076] (2) ZmPRR7-ZHENG58: forward: 5'-T...

Embodiment 3

[0084] 1. Gene sequence:

[0085] First, use the genomic DNA sequences of the ZmZCN8 genes of the maize varieties CML166 and Zheng 58 obtained by cloning in the present invention, and use the software Mega4.0 to compare the nucleic acid sequences to obtain a sequence alignment.

[0086] 2, with the second step of embodiment 1.

[0087] 3. Design primers based on the differences in known related sequences:

[0088] Comparing the partial DNA sequences of CML166 and Zheng 58 of the ZmZCN8 gene with the software Mega4.0, the DNA sequence of the ZmZCN8 gene of CML166 is compared with the sequence of Zheng 58, and one of them has a transversion: the base of CML166 is A, while the DNA sequence of Zheng 58 The base of 58 is G. Use the characteristics of sequence base transversion to design primers:

[0089] (1) ZmZCN8-CML166: Forward: 5'-GAGAGTTCTAATAAGAGCAACGA-3

[0090] Reverse: 5'-TGCAAGATTTAGGCATTTTGTTC-3'

[0091] (2) ZmZCN8-ZHENG58: Forward: 5'-GAGAGTTCTAA...

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Abstract

The invention relates to the field of molecular markers, and in particular relates to a corn photoperiod gene specific molecular marker and an application thereof. Part of genomic deoxyribose nucleic acid (DNA) sequences of three corn photoperiod gene regulation candidate genes of ZmPRR7, ZmZCN8 and ZmPIF3 in a tropical selfing line CML166 and a temperate selfing line Zheng58 are compared and identified to design three different primers, wherein one primer is a general primer which is designed according to the same sequence of the two selfing lines, the other two primers are Zheng58 or CML166 specific PCR3' primers which are designed by utilizing the single base difference of the two corn selfing lines and can be respectively paired with the general primer, and target strips can be amplified in one selfing line of CML166 or Zheng 58 or the hybrid F1 of the CML166*the Zheng 58 of each pair of primers and are same in size. When a tropical idioplasm is utilized for idioplasm improvement, selfing line breeding or corn hybrid commodification seed production, the markers can be utilized for assist selective breeding and guide actual production.

Description

technical field [0001] The invention relates to the field of molecular markers, in particular to specific molecular markers of maize photoperiod genes and applications thereof. Background technique [0002] As an important grain, feed and economic ternary crop, corn plays a pivotal role in agricultural production and national economy. The tropical germplasm resources from the Maize Diversity Center have rich types of genetic variation, many of which are excellent traits (such as stress resistance, disease resistance, insect resistance, etc.) that temperate maize does not have. Utilizing tropical and subtropical maize germplasm in temperate zone breeding can broaden the germplasm base of temperate zone maize, improve the variety's resistance to stress, disease and insect pests, create new germplasm, and establish a new heterosis model. However, photoperiod sensitivity limits the utilization of tropical and subtropical maize germplasm in temperate regions and the quality and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 张素芝邱林权王雷王娜王红玲梁晓玲黄凤珠覃小俏徐栓栓
Owner SICHUAN AGRI UNIV
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