ZmCLA1 gene capable of controlling sizes of included angles of maize leaves as well as method and application of ZmCLA1 gene in breeding high-density-tolerant maize

A corn leaf angle and gene technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of insufficient number of genes, difficult to achieve sustained high yield of corn, and molecular biological functions have not yet been reported, etc. Small, compact plant size

Active Publication Date: 2015-04-01
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0003] From the beginning of the 20th century to the present, maize genetic improvement aimed at breeding new varieties by using heterosis has always been an important direction of agricultural scientific research in countries all over the world. It is Difficult to Realize the Goal of Sustained High Yield of Maize
Compared with the identified QTL, the number of identified genes related to leaf

Method used

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  • ZmCLA1 gene capable of controlling sizes of included angles of maize leaves as well as method and application of ZmCLA1 gene in breeding high-density-tolerant maize
  • ZmCLA1 gene capable of controlling sizes of included angles of maize leaves as well as method and application of ZmCLA1 gene in breeding high-density-tolerant maize
  • ZmCLA1 gene capable of controlling sizes of included angles of maize leaves as well as method and application of ZmCLA1 gene in breeding high-density-tolerant maize

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Embodiment Construction

[0037] The present invention utilizes the compact maize inbred line Yu 82 ( figure 1 ) Is the female parent, the loose maize inbred line Shen137 (pictured) bred by Shenyang Academy of Agricultural Sciences is used as the male parent to obtain F1, F1 self-crossed to obtain F2 mapping population and its derived F2:3 family as the positioning population. Through the identification of leaf angle phenotypes at 3 locations, QTL mapping of leaf angle was carried out, and 3 QTLs related to leaf angle were co-located, and the main effect qLA1 located on the first chromosome had the largest effect value (Ku et al. 2010). To this end, the inbred line Yu 82 was used as the donor parent and the inbred line Shen 137 was used as the recipient parent to construct a near-isogenic line of qLA1. It was constructed by backcrossing and breeding combined with molecular marker-assisted selection. The constructed near-isogenic line is Shen137-NIL68 ( figure 1 ) To further understand the content and ...

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Abstract

The invention discloses a ZmCLA1 gene being capable of controlling the sizes of the included angles of maize leaves and having nucleotide sequence as shown in SEQ ID NO: 1. By a forward genetic method (in which by building a mapping population, the included angle of maize leaves is subjected to QTL primary positioning and fine positioning), candidate genes are unmistakably separated and the function of the ZmCLA1 gene in plants is not reported; the invention provides the ZmCLA1 gene capable of controlling the sizes of the included angles of maize leaves and a protein encoded by the ZmCLA1 gene. The ZmCLA1 gene has important value for the theoretical study on analysis of molecular mechanism for forming the included angles among maize leaves, the ZmCLA1 gene containing the sequence as shown in SEQ ID NO: 1 is modified and inoculated onto maize plants so that the maize breeding material which has compact plant type and small included angles of leaves can be created and the ZmCLA1 gene has important practical significance for breeding maize.

Description

technical field [0001] The invention relates to the technical field of maize breeding, in particular to a ZmCLA1 gene controlling the angle between maize leaves and its method and application in breeding dense-strain-resistant maize. Background technique [0002] Corn is the second largest grain crop in my country, with an annual planting area of ​​about 25.45 million hectares, and the sown area and total output account for about 25% and 28% of the grain crops. With the reduction of arable land and the continuous increase of population, the consumption of corn shows a rigid increase. Therefore, increasing the yield per unit area and increasing the total yield is an important goal for the long-term pursuit of maize breeding in my country. Research at home and abroad shows that increasing the yield per unit area is the main measure to develop corn production, and the improvement of per unit area yield mainly depends on the selection and renewal of new varieties. Maize variet...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H1/02A01H5/00
Inventor 陈彦惠库丽霞郭书磊
Owner HENAN AGRICULTURAL UNIVERSITY
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