ZmCLA1 gene capable of controlling sizes of included angles of maize leaves as well as method and application of ZmCLA1 gene in breeding high-density-tolerant maize

Abstract

The invention discloses a ZmCLA1 gene being capable of controlling the sizes of the included angles of maize leaves and having nucleotide sequence as shown in SEQ ID NO: 1. By a forward genetic method (in which by building a mapping population, the included angle of maize leaves is subjected to QTL primary positioning and fine positioning), candidate genes are unmistakably separated and the function of the ZmCLA1 gene in plants is not reported; the invention provides the ZmCLA1 gene capable of controlling the sizes of the included angles of maize leaves and a protein encoded by the ZmCLA1 gene. The ZmCLA1 gene has important value for the theoretical study on analysis of molecular mechanism for forming the included angles among maize leaves, the ZmCLA1 gene containing the sequence as shown in SEQ ID NO: 1 is modified and inoculated onto maize plants so that the maize breeding material which has compact plant type and small included angles of leaves can be created and the ZmCLA1 gene has important practical significance for breeding maize.

Description

technical field [0001] The invention relates to the technical field of maize breeding, in particular to a ZmCLA1 gene controlling the angle between maize leaves and its method and application in breeding dense-strain-resistant maize. Background technique [0002] Corn is the second largest grain crop in my country, with an annual planting area of ​​about 25.45 million hectares, and the sown area and total output account for about 25% and 28% of the grain crops. With the reduction of arable land and the continuous increase of population, the consumption of corn shows a rigid increase. Therefore, increasing the yield per unit area and increasing the total yield is an important goal for the long-term pursuit of maize breeding in my country. Research at home and abroad shows that increasing the yield per unit area is the main measure to develop corn production, and the improvement of per unit area yield mainly depends on the selection and renewal of new varieties. Maize variet...

AI Technical Summary

Problems solved by technology

[0003] From the beginning of the 20th century to the present, maize genetic improvement aimed at breeding new varieties by using heterosis has always been an important direction of agricultural scientific research in countries all over the world. It is Difficult to Realize the Goal of Sustained High Yield of Maize

Compared with the identified QTL, the number of identified genes related to leaf angle is far from enough, indicating that a large number of candidate genes related to leaf angle need to be further identified

The gene ZmCLA1 involved in this study is a new gene containing GHMP-kinases-N and Pmev-kin-ERG8 domains related to the size of the leaf angle discovered by map-based cloning technology, and its molecular biological function has not yet been found in plants. to report

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