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A kind of zmcla2-1 gene that regulates the included angle of corn leaves and its application

A technology of corn leaf angle and genetics, applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effect of compact plant type and small leaf angle

Active Publication Date: 2021-09-24
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a method for regulating the included angle of corn leaves. ZmCLA2-1 The gene and its application solve the problems encountered in the cultivation of excellent high-yielding maize varieties with dense plant type and dense tolerance characteristics in the prior art

Method used

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  • A kind of zmcla2-1 gene that regulates the included angle of corn leaves and its application
  • A kind of zmcla2-1 gene that regulates the included angle of corn leaves and its application
  • A kind of zmcla2-1 gene that regulates the included angle of corn leaves and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Construction of near-isogenic lines

[0030] The maize inbred line Yu 82 with small leaf angle and compact plant type was used as the donor parent, and the maize inbred line Yu 87-1 with large leaf angle and loose plant type was used as the recipient parent through backcross transfection combined with molecular markers Assisted selection technique to construct near-isogenic lines ( figure 1 ). In the spring of 2006, the combination of two parents in Zhengzhou won F 1 ; F planted in Hainan in the winter of 2007 1 Progeny and recipient parent Yu 87-1, backcross F with Yu 87-1 1 get BC 1 f 1 ;according to BC 1 f 1 The phenotype selection of 21 plants with small leaf angles was planted in Zhengzhou in the spring of 2008 to form ear rows, and continued to be backcrossed with Yu 87-1 to obtain BC 2 f 1 ; Selected 18 individual plants with small leaf angles and planted them in Sanya, Hainan in the winter of 2008, and then continued to backcross with Yu 87-...

Embodiment 2

[0031] Embodiment 2: Adopt map bit cloning technology to separate ZmCLA2-1 Gene

[0032] Use BC 3 f 2 The population of 6529 strains was used as a fine-positioning population, and the single strain 720 with a leaf angle less than or equal to 15° was selected from it. QLA1 Two-sided markers umc1165 and bnlg1297 were used to analyze their genotypes, and 18 individual plants with recombination and exchange at umc1165 or bnlg1297 were selected; at the same time, 608 individual plants with leaf angles greater than 20° were selected, and 5 were screened out by combining molecular marker selection. Exchange individual plants with large leaf angles. Using newly developed 17 pairs of primers SSR2-1, SSR2-2, SSR2-3, SSR2-4, SSR2-5, SSR2-6, SSR2-24, SSR2-25, SSR2-26, SSR2 with strong polymorphism -27, SSR2-28, SSR2-36, SSR2-37, SSR2-38, SSR2-39, SSR2-40, SSR2-54 (marker development methods refer to Zhang Jun, ZmCLA4 gene map-site cloning and Functional Analysis, Ph.D. Dissertation ...

Embodiment 3

[0035] Example 3: ZmCLA2-1 Negative regulation of maize leaf angle

[0036] ZmCLA2-1 The leaf angle was increased in Yu87-1, and the leaf angle was decreased in Yu87-1-NIL58, so we detected the expression of ZmCLA2-1 gene. The results of qRT-PCR detection showed that the leaves from the 10 to 12 leaf stages of maize growth and development ( image 3 ) efficient expression. For different materials, ZmCLA2-1 The expression level of Yu87-1 in different periods was significantly lower than that of Yu87-1-NIL58 ( image 3 ),show ZmCLA2-1 The leaf angle of maize with high expression level is relatively reduced, so it is proposed that ZmCLA2-1 Regulates the size of the maize leaf angle as a negative regulator.

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Abstract

The invention relates to the field of crop genetics and breeding, in particular to a method for regulating the included angle of corn leaves. ZmCLA2-1 Genes and their applications. The present invention utilizes overexpression technology to promote endogenous corn ZmCLA2-1 The expression of the gene resulted in well-developed supporting tissue at the distal end of the leaf occipital, a high degree of lignification, more layers of sclerenchyma cells, smaller and more cells, and increased vascular bundles, resulting in a decrease in the leaf angle. Specifically, the ZmCLA2-1 The gene is fused with a constitutive promoter to construct a vector that promotes gene expression, and the inbred line of maize with small leaf angle and compact plant type is created through transgenic technology, which is used to breed new maize varieties resistant to denseness. The invention transforms the gene containing the sequence shown in SEQ ID NO: 1 and introduces it into corn plants to create corn breeding materials with compact plant shape and small leaf angle, which has important practical application significance for corn breeding.

Description

technical field [0001] The invention relates to the field of crop genetics and breeding, in particular to a method for regulating the included angle of corn leaves. ZmCLA2-1 Genes and their applications. Background technique [0002] In recent years, the area of ​​arable land has gradually decreased, and factors such as the balance between the planting area of ​​various crops and actual demand have also led to the reduction of the planting area of ​​corn. However, the corn required for national development continues to increase. On the limited arable area, only by increasing the yield per unit area and then increasing the total yield can meet social needs and ensure national food security. Increasing the planting density by cultivating good density-tolerant maize varieties is proposed as a method to increase the yield per unit area. The new high-yield maize varieties with tolerance to density must have the plant type and characteristics of tolerance to density. A compact ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/12A01H6/46
CPCC07K14/415C12N15/8205C12N15/8261
Inventor 陈彦惠库丽霞曹莹莹任真真
Owner HENAN AGRICULTURAL UNIVERSITY
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