A method for breeding early flowering maize by using maize zmdps10-2 gene and its application

Abstract

The invention belongs to the field of crop genetics and breeding, and relates to a method for breeding early-blossoming corn by adopting corn gene ZmDPS10-2 and applications of the method. The method comprises the following steps: by taking a selfing line CML288 being sensitive to the photoperiod and having late blossoming as a donor parent, taking a selfing line Huangzao 4 insensitive to the photoperiod and having early blossoming as a receptor parent, and adopting a method combining backcross transformation and molecular markers, carrying out assistant selection for constructing a near-isogenic line Huangzao 4-NIL of major qDPS10-2 positioned on the tenth chromosome; and separating the ZmDPS10-2 gene by adopting a map-based cloning technology, increasing the expression of the corn endogenous ZmDPS10-2 gene by adopting a transgenic overexpression technology, and breeding the early-blossoming selfing line of corn under the long-day sunshine condition. With the adoption of the scheme, the molecular biological function of the gene in regulating the corn blossoming time under the long-day sunshine condition is researched, and a potential novel gene resource is provided for culturing the excellent high-yield variety of corn realizing early-blossoming under the long-day sunshine condition.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a method for breeding early-flowering corn by using the corn ZmDPS10-2 gene and its application. Background technique [0002] At present, the germplasm sources for maize breeding and production in my country are mainly concentrated in the four traditional types of Rhett, Lancaster from the United States, Tang Sipingtou and Luda Honggu in China, and a newer germplasm type P group. Especially after the success of Zhengdan 958 and Xianyu 335, domestic breeding was further limited to improving the parents of these two hybrids, which narrowed the germplasm resources for breeding. The narrow genetic basis not only becomes the main obstacle to the improvement of corn yield and quality, but also increases the possibility of maize hybrids being attacked by diseases and insect pests, making corn production fall into the danger of genetic vulnerability. [0003] Exotic germplasm, e...

AI Technical Summary

Problems solved by technology

However, tropical and subtropical maize germplasms are located in low latitudes for a long time and have strong photoperiod sensitivity. In temperate regions, under long-day sunshine conditions, they show vigorous vegetative growth, inhibited reproductive growth, delayed tasseling and silking stages, and late maturity. , uncoordinated male and female, even unable to flower and bear fruit

Photoperiod sensitivity severely limits the utilization of tropical maize in temperate regions (Holland and Goodman 1995)

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