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The zmcla1 gene controlling the size of the included angle of maize leaves and its method and application in breeding dense plant-tolerant maize

A corn leaf angle and gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of insufficient number of genes, difficult to achieve sustained high yield of corn, and molecular biological functions have not yet been reported, etc. Small, compact plant size

Active Publication Date: 2017-06-16
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] From the beginning of the 20th century to the present, maize genetic improvement aimed at breeding new varieties by using heterosis has always been an important direction of agricultural scientific research in countries all over the world. It is Difficult to Realize the Goal of Sustained High Yield of Maize
Compared with the identified QTL, the number of identified genes related to leaf angle is far from enough, indicating that a large number of candidate genes related to leaf angle need to be further identified
The gene ZmCLA1 involved in this study is a new gene containing GHMP-kinases-N and Pmev-kin-ERG8 domains related to the size of the leaf angle discovered by map-based cloning technology, and its molecular biological function has not yet been found in plants. to report

Method used

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  • The zmcla1 gene controlling the size of the included angle of maize leaves and its method and application in breeding dense plant-tolerant maize
  • The zmcla1 gene controlling the size of the included angle of maize leaves and its method and application in breeding dense plant-tolerant maize
  • The zmcla1 gene controlling the size of the included angle of maize leaves and its method and application in breeding dense plant-tolerant maize

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Embodiment Construction

[0037] The present invention utilizes the compact corn inbred line Yu 82 ( figure 1 ) as the female parent, and the loose maize inbred line Shen 137 (pictured) selected by Shenyang Academy of Agricultural Sciences as the male parent was crossed to obtain F1, and F1 was self-crossed to obtain F2 mapping population and its derived F2:3 family as the positioning population, Through the identification of leaf angle phenotypes in three locations, QTL mapping was carried out for leaf angle, and three QTLs related to leaf angle were co-localized, among which the main effect qLA1 located on the first chromosome had the largest effect value (Ku et al. 2010). Therefore, a near-isogenic line of qLA1 was constructed with the inbred line Yu 82 as the donor parent and the inbred line Shen 137 as the recipient parent. It was constructed by backcrossing combined with molecular marker-assisted selection, and the near-isogenic line constructed was Shen 137-NIL68 ( figure 1 ), in order to fu...

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Abstract

The invention discloses a ZmCLA1 gene controlling the size of the included angle of maize leaves, the nucleotide sequence of which is shown in SEQ ID NO:1. The present invention adopts the forward genetics method (preliminary determination of QTL and fine positioning of the leaf angle by constructing a mapping population) to accurately isolate candidate genes. The function of the ZmCLA1 gene has not been reported in plants. The present invention provides regulation Maize leaf angle size gene ZmCLA1 and its encoded protein. This gene is of great value for theoretical studies such as analyzing the molecular mechanism of the formation of corn leaf angles. The gene containing the sequence shown in SEQ ID NO: 1 is transformed and introduced into maize plants to create compact plants with small leaf angles. The maize breeding materials have important practical significance for maize breeding.

Description

technical field [0001] The invention relates to the technical field of maize breeding, in particular to a ZmCLA1 gene controlling the angle between maize leaves and its method and application in breeding dense-strain-resistant maize. Background technique [0002] Corn is the second largest grain crop in my country, with an annual planting area of ​​about 25.45 million hectares, and the sown area and total output account for about 25% and 28% of the grain crops. With the reduction of arable land and the continuous increase of population, the consumption of corn shows a rigid increase. Therefore, increasing the yield per unit area and increasing the total yield is an important goal for the long-term pursuit of maize breeding in my country. Research at home and abroad shows that increasing the yield per unit area is the main measure to develop corn production, and the improvement of per unit area yield mainly depends on the selection and renewal of new varieties. Maize variet...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H1/02A01H5/00
Inventor 陈彦惠库丽霞郭书磊
Owner HENAN AGRICULTURAL UNIVERSITY
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