A method for preparing a conjugated oligomer / gold nanocluster light energy conversion material

Abstract

The application belongs to the field of optical functional composite material application, and discloses a preparation method of conjugated oligomer / gold nanocluster light energy conversion material. The preparation method is to synthesize conjugated oligomer material with main chain of fluorene and thiophene and side chain containing active groups, and to compound the conjugated oligomer material with gold nanocluster containing mercapto ligand through covalent connection to prepare conjugated oligomer / gold nanocluster compound. The preparation method is simple and easy to operate, and can effectively compound two kinds of semiconductor materials. The nanocomposite has a size of about 2 nanometers and has the ability to quickly enter photosynthetic organisms and structures. The conjugated oligomer / gold nanocluster heterojunction is cultured with photosynthetic organisms and structures, and under sunlight irradiation, high-efficiency photo-generated electrons are generated and quickly transferred to the electron transport chain of the photosynthetic organisms and structures, so as to improve the solar energy conversion efficiency, increase the biomass yield, and have practical application value in the field of energy conversion.

Description

TECHNICAL FIELD

[0001] The application belongs to the field of optical functional composite materials, and relates to a preparation method of a conjugated oligomer / gold nanocluster heterojunction light energy conversion material. BACKGROUND

[0002] Solar energy is a kind of abundant renewable energy. The photosynthesis in nature can realize the utilization and storage of solar energy. In photosynthetic organisms, light can drive electron transfer to produce biological electricity and biological fuel and other biological energy. However, the solar energy conversion efficiency of photosynthetic organisms is extremely low, about 3%, and the photosynthesis is actually difficult to meet the existing biological energy demand.

[0003] At present, the solar energy conversion efficiency of photosynthetic organisms is mainly improved by genetic engineering and introduction of artificial semiconductor materials. For example, overexpression of the “VPZ” structure (containing three genes related to the xanthophyll cycle) in soybean can accelerate the switching process of the plant xanthophyll cycle, significantly improve the photosynthesis efficiency, and increase the yield by more than 20% in field tests. However, due to the adaptability difference between different species and the complex regulation process, the genetic modification technology is difficult to implement and lacks universality. Artificial synthetic materials, such as organic semiconductor materials, have strong light absorption performance, which expands the light capture range of cyanobacteria and accelerates the electron transfer rate in the light reaction. The yield of oxygen, NADPH and ATP in the light-dependent reaction is increased by 52.8%, 47.9% and 27.2%, respectively. Inorganic nanomaterials can also be used to simulate natural enzymes and participate in photosynthesis. Although artificial synthetic materials have potential in optimizing photosynthesis, they are difficult to efficiently enter the cells of photosynthetic organisms and directly enter the biosynthesis center, which limits the solar energy conversion efficiency.

[0004] The artificial heterojunction nanomaterials developed based on gold nanoclusters and organic semiconductor materials have structural and functional designability. Small-sized nanoclusters can quickly enter cells, thereby directly transferring exogenous photo-generated electrons to the inside of photosynthetic organisms and directly participating in photoelectric conversion. How to utilize this characteristic to prepare nanoheterojunction materials with high solar energy conversion efficiency and stable performance is a technology that needs to be developed at present, and has practical value for the application of nanocomposites in the development and utilization of biomass energy. SUMMARY

[0005] The purpose of the present application is to solve the problem of low solar energy conversion efficiency of photosynthetic organisms, and provide a preparation method of a conjugated oligomer / gold nanocluster heterojunction light energy conversion material, which can be used to improve the solar energy conversion efficiency of photosynthetic organisms and increase the biomass yield.

[0006] The conjugated oligomer / gold nanocluster photoenergy conversion material is prepared by synthesizing a conjugated oligomer material with a main chain of fluorene and thiophene and a side chain containing an active group, and then compounding the conjugated oligomer material with a gold nanocluster containing a mercapto ligand through covalent connection.

[0007] The specific steps of the present application are as follows:

[0008] (1) Synthesize a conjugated oligomer material with a main chain of fluorene and thiophene and a side chain containing an active group R, dissolve the conjugated oligomer in deionized water at a mass ratio of 1:10000-1:1000, and uniformly stir and ultrasonically disperse to obtain a conjugated oligomer solution;

[0009] (2) Mix and stir a phosphorl tetrahydroxy methyl chloride solution, a chloroauric acid solution, and a sodium hydroxide solution (pH=8-11) at a volume ratio of 180:110:8000-180:150:8000, then mix and stir the above solution with a mercapto ligand solution at a volume ratio of 1:100-1:50, and stand still at 2-6℃ for 24-48 hours to prepare a gold nanoparticle solution;

[0010] (3) Mix and stir the gold nanoparticle solution obtained in step (2) with a sodium tetraborate solution and a mercapto undecanoic acid ethanol solution at a volume ratio of 20:8:3-100:8:3, and perform ultrafiltration in an ultrafiltration tube to obtain a gold nanocluster solution;

[0011] (4) Mix and stir the gold nanocluster solution obtained in step (3), a 1-ethyl-(3-dimethylaminopropyl) carbonyl diimide solution, and an N-hydroxyl thiosuccinimide solution at a volume ratio of 100:50:50-100:1:1;

[0012] (5) Mix and stir the solution obtained in step (4) with the oligomer solution obtained in step (1) at a volume ratio of 1:1-10:1 after ultrafiltration in an ultrafiltration tube, and perform ultrafiltration in an ultrafiltration tube to obtain a conjugated oligomer / gold nanocluster photoenergy conversion material.

[0013] Further, the main chain in step (1) is one of the main chain structures of formulas ①-④, wherein the number of m is 0-10, and the number of n is 0-10.

[0014]

[0015] Further, the active group R in step (1) is one or both of a carboxyl group and an amino group.

[0016] Further, the mercapto ligand in step (2) is one or more of mercaptobenzoic acid, mercaptopropionic acid, mercaptohexanoic acid, disulfide ligand, mercaptoethylene glycol, thiophene derivative, bis-mercaptoethane, polythiol compound, mercaptoethanol, mercapto-p-toluenesulfonic acid, mercapto-trivinyltetramine, glutathione.

[0017] Further, the mass fraction of the tetramethylammonium chloride solution in step (2) is 0.5-5%, the concentration of chloroauric acid is 10-200 mM, and the concentration of the mercapto ligand solution is 0.5-20 mM.

[0018] Further, the concentration of the sodium tetraborate solution in step (3) is 1-25 mM, and the concentration of the mercapto-undecanoic acid ethanol solution is 0.01-0.2 mM.

[0019] Further, the concentration of the 1-ethyl-(3-dimethylaminopropyl) carbonyl diimide solution in step (4) is 0.01-0.1 mM, and the concentration of the N-hydroxysuccinimide solution is 0.02-0.08 mM.

[0020] Further, the molecular weight cut-off of the ultrafiltration tube in steps (3) and (5) is 300-10000, the ultrafiltration time is 0.1-0.5 hours, and the ultrafiltration centrifugal force is 2000-6000 g.

[0021] Further, the mixing and stirring time in step (2) is 10-30 minutes, the mixing and stirring time in step (3) is 12-48 hours, the mixing and stirring time in step (4) is 10-30 minutes, and the mixing and stirring time in step (5) is 0.5-24 hours.

[0022] An application of a conjugated oligomer / gold nanocluster light energy conversion material. The conjugated oligomer / gold nanocluster light energy conversion material prepared above is co-cultured with photosynthetic organisms in a good solvent according to a mass ratio of 1:400-1:10. After sufficient action, the solar energy conversion efficiency is significantly improved, and the photosynthesis of biomass is promoted.

[0023] Further, the photosynthetic organisms are one or more of cyanobacteria, red algae, cryptomonads, dinoflagellates, chrysophytes, yellow algae, diatoms, brown algae, euglenoids, green algae, desmids, photosynthetic bacteria, and higher plants.

[0024] Further, the good solvent is one or more of deionized water, phosphate buffer, Tris buffer, agarose suspension, M9 buffer, LB buffer, normal saline, BG11 medium, F / 2 medium, Walne medium, Provasoli medium, ASM-1 medium, Z8 medium, K medium, MS medium, B5 medium, White's medium, SH medium, WPM medium, NN medium, and Anderson's medium.

[0025] Further, the number of days for the co-culture in the good solvent is 0.5-7 days.

[0026] The conjugated oligomer / gold nanocluster photoenergy conversion material obtained by the method has effective photo-generated carrier separation efficiency, and after entering into photosynthetic organisms and structures, photo-generated electrons can be quickly transferred to photosynthetic electron transport chains, thereby improving solar energy conversion efficiency and increasing biomass yield.

[0027] The technical features of the present application are:

[0028] 1. By adjusting the number of thiophene and fluorene contained in the main chain of the conjugated oligomer, the type of active groups contained in the side chain of the conjugated oligomer, and the type of mercapto ligands of the gold nanocluster, covalent connection of the conjugated oligomer and the gold nanocluster can be successfully achieved, and the conjugated oligomer / gold nanocluster heterojunction photo-generated carrier separation characteristics are endowed.

[0029] 2. By controlling the volume ratio of the gold nanocluster solution, the 1-ethyl-(3-dimethylaminopropyl) carbodiimide solution and the N-hydroxysuccinimide solution, the concentration of the 1-ethyl-(3-dimethylaminopropyl) carbodiimide solution and the N-hydroxysuccinimide solution, and the volume ratio of the above mixed solution and the conjugated oligomer solution, different conjugated oligomer / gold nanocluster heterojunctions can be obtained. The two semiconductor materials are effectively compounded, the size of the nanocomposite is about 2 nanometers, and the nanocomposite has the ability to quickly enter photosynthetic organisms and structures, and exhibits good photo-generated electron performance.

[0030] Compared with the prior art, the present application has the following advantages and benefits: by changing the main chain structure of the conjugated oligomer, the utilization efficiency of the conjugated oligomer for solar energy is effectively increased; by covalent connection of the conjugated oligomer and the gold nanocluster to form a composite heterojunction, the photo-generated electron-hole separation efficiency is improved, and the excited state lifetime of the gold nanocluster is effectively improved. By co-culturing the conjugated oligomer / gold nanocluster heterojunction with photosynthetic organisms, the conjugated oligomer / gold nanocluster heterojunction can generate photo-generated electrons and transfer them to photosynthetic organisms and structures, inject external electrons to accelerate synthetic metabolic reactions, thereby improving the production rate and yield of biomass, and the preparation method is simple and easy to implement, which has practical application value in the field of energy conversion. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 is a transmission electron microscope (TEM) photo of the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1.

[0032] Figure 2 is a transmission electron microscope (TEM) photo of the section of cyanobacteria after the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 was co-cultured with the cyanobacteria.

[0033] Figure 3 is a transient photocurrent density curve of the cyanobacteria after the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 was co-cultured with the cyanobacteria.

[0034] Figure 4 is a column chart of the lipid content of the cyanobacteria before and after the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 was co-cultured with the cyanobacteria. DETAILED DESCRIPTION

[0035] Example 1

[0036] (1) A conjugated oligomer material with a main chain of fluorene-thiophene-fluorene and thiophene side chains containing amino groups and fluorene side chains containing carboxyl groups was dissolved in deionized water at a mass ratio of 1:5000, stirred uniformly, and then ultrasonically dispersed to obtain a conjugated oligomer solution;

[0037]

[0038] (2) A 1% tetramethylammonium chloride solution, a 50mM chloroauric acid solution, and a sodium hydroxide solution (pH=9) were mixed at a volume ratio of 180:137:8000, stirred for 15 minutes, and then mixed with a 10 mM mercaptobenzoic acid solution at a volume ratio of 1:80, stirred for 15 minutes, and then allowed to stand at 4°C for 24 hours to prepare a gold nanoparticle solution;

[0039] (3) The gold nanoparticle solution obtained in step (2) was mixed with a 5mM sodium tetraborate solution and a 0.1mM mercaptoundecanoic acid ethanol solution at a volume ratio of 40:8:3, stirred for 24 hours, and then subjected to ultrafiltration in an ultrafiltration tube with a molecular weight cutoff of 3000 at a centrifugal force of 4000g for 20 minutes to obtain a gold nanocluster solution;

[0040] (4) The gold nanocluster solution obtained in step (3), a 0.04 mM 1-ethyl-(3- dimethylaminopropyl) carbonyl diimide solution, and a 0.02 mM N-hydroxysuccinimide solution were mixed at a volume ratio of 100:10:10 and stirred for 15 minutes;

[0041] (5) The solution obtained in step (4) is mixed with the oligomer solution obtained in step (1) at a volume ratio of 4:1, stirred for 4 hours, and then ultrafiltered in an ultrafiltration tube with a molecular weight cut-off of 3000 at a centrifugal force of 4000 g for 10 minutes to obtain a conjugated oligomer / gold nanocluster heterojunction, i.e., a conjugated oligomer / gold nanocluster light energy conversion material.

[0042] (6) The conjugated oligomer / gold nanocluster heterojunction is co-cultured with cyanobacteria in deionized water at a mass ratio of 1:25 for 7 days.

[0043] Performance test:

[0044] Figure 1 is a transmission electron microscope (TEM) photo of the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1. According to Figure 1 It can be seen that the particle size of the heterojunction is uniform, about 2 nm, and well dispersed.

[0045] Figure 2 is a transmission electron microscope (TEM) photo of a blue algae section after co-culturing the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 with blue algae. According to Figure 2 It can be seen that the conjugated oligomer / gold nanocluster heterojunction can enter the inside of the blue algae and has good dispersibility in the cells.

[0046] Figure 3 is a transient photocurrent density curve of blue algae after co-culturing the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 with blue algae. According to Figure 3 It can be seen that after co-culturing with the conjugated oligomer / gold nanocluster heterojunction, the photocurrent of the blue algae reached 50 nA / cm 2 , confirming that the conjugated oligomer / gold nanocluster heterojunction can improve the light energy conversion efficiency of the blue algae.

[0047] Figure 4 is a lipid content column chart of blue algae before and after co-culturing the conjugated oligomer / gold nanocluster heterojunction prepared in Example 1 with blue algae. According to Figure 4 It can be seen that after co-culturing with the conjugated oligomer / gold nanocluster heterojunction, the lipid content of the blue algae increased by 19.26%, promoting the increase of the biomass yield of the blue algae.

[0048] Example 2

[0049] Steps (1)-(5) are the same as in Example 1;

[0050] Step (6) is to co-culture the conjugated oligomer / gold nanocluster heterojunction and blue algae in deionized water at a mass ratio of 1:25 for 7 days. The lipid content of green algae increases by 16.74%, and the protein content increases by 27.25%, promoting the increase of green algae biomass yield.

[0051] Example 3

[0052] (1) Synthesis of conjugated oligomer material with fluorene-thiophene-thiophene-fluorene as main chain, thiophene side chain containing amino group and fluorene side chain containing carboxyl group, dissolve the conjugated oligomer in deionized water at a mass ratio of 1:4000, stir uniformly, and then ultrasonic dispersion to obtain a conjugated oligomer solution;

[0053]

[0054] (2) Mix 1% tetramethylammonium chloride solution, 50mM chloroauric acid solution and sodium hydroxide solution (pH=9) at a volume ratio of 180:137:8000, stir for 15 minutes, then mix the above solution with 10 mM glutathione solution at a volume ratio of 1:80, stir for 15 minutes, and stand at 4°C for 24 hours to prepare a gold nanoparticle solution;

[0055] (3) Mix the gold nanoparticle solution obtained in step (2) with 5mM sodium tetraborate solution and 0.1mM mercaptoundecanoic acid ethanol solution at a volume ratio of 50:8:3, stir for 48 hours, and then centrifuge at 4000g for 20 minutes in an ultrafiltration tube with a molecular weight cutoff of 3000 to obtain a gold nanocluster solution;

[0056] (4) Mix the gold nanocluster solution obtained in step (3), 0.04 mM 1-ethyl-(3-dimethylaminopropyl) carbonyl diimide solution and 0.02 mM N-hydroxysuccinimide solution at a volume ratio of 100:20:20, stir for 15 minutes;

[0057] (5) Mix the solution obtained in step (4) with the oligomer solution obtained in step (1) at a volume ratio of 2:1, stir for 3 hours, and then centrifuge at 4000g for 10 minutes in an ultrafiltration tube with a molecular weight cutoff of 3000 to obtain a conjugated oligomer / gold nanocluster heterojunction, i.e. a conjugated oligomer / gold nanocluster light energy conversion material;

[0058] (6) Co-culture the conjugated oligomer / gold nanocluster heterojunction and blue algae in deionized water at a mass ratio of 1:10 for 7 days. The blue algae photoelectric current reaches 52 nA / cm 2, and the lipid content of cyanobacteria increased by 19.98%, which promoted the increase of cyanobacterial biomass production.

[0059] Example 4

[0060] Steps (1)-(5) are the same as Example 3;

[0061] Step (6) is to co-culture the conjugated oligomer / gold nanocluster heterojunction and cyanobacteria in deionized water at a mass ratio of 1:50 for 7 days. The photocurrent of cyanobacteria reached 43 nA / cm 2 , and the lipid content of cyanobacteria increased by 16.12%, which promoted the increase of cyanobacterial biomass production.

[0062] Example 5

[0063] (1) Synthesis of conjugated oligomer material with thienyl-fluorenyl-thienyl main chain, thienyl side chain containing amino group, and fluorenyl side chain containing carboxyl group, dissolve the conjugated oligomer in deionized water at a mass ratio of 1:6000, stir uniformly, and then ultrasonic dispersion to obtain a conjugated oligomer solution;

[0064]

[0065] (2) Mix 1% tetrahydroxymethyl phosphonium chloride solution, 50mM chloroauric acid solution and sodium hydroxide solution (pH=9) at a volume ratio of 180:137:8000, stir for 20 minutes, then mix the above solution with 10 mM mercaptobenzoic acid solution at a volume ratio of 1:80, stir for 20 minutes, and stand at 4℃ for 30 hours to prepare a gold nanoparticle solution;

[0066] (3) Mix the gold nanoparticle solution obtained in step (2) with 5mM sodium tetraborate solution and 0.1mM mercaptoundecanoic acid ethanol solution at a volume ratio of 40:8:3, stir for 48 hours, and then centrifuge at 4000g for 20 minutes in an ultrafiltration tube with a molecular weight cutoff of 3000 to obtain a gold nanocluster solution;

[0067] (4) Mix the gold nanocluster solution obtained in step (3), 0.04 mM 1-ethyl-(3-dimethylaminopropyl) carbonyldiimide solution and 0.02 mM N-hydroxysuccinimide solution at a volume ratio of 100:5:5, and stir for 15 minutes;

[0068] (5) The solution obtained in step (4) is mixed with the oligomer solution obtained in step (1) at a volume ratio of 4:1 and stirred for 5 hours, and then ultrafiltrated in an ultrafiltration tube with a molecular weight cut-off of 3000 at a centrifugal force of 4000 g for 10 minutes to obtain a conjugated oligomer / gold nanocluster heterojunction, i.e., a conjugated oligomer / gold nanocluster light energy conversion material;

[0069] (6) The conjugated oligomer / gold nanocluster heterojunction is co-cultured with cyanobacteria in deionized water at a mass ratio of 1:25 for 7 days. The photocurrent of the cyanobacteria reaches 37 nA / cm 2 , improving the light energy conversion efficiency of the cyanobacteria. The lipid content of the cyanobacteria increases by 14.06%, promoting the increase of cyanobacterial biomass production.

[0070] Example 6

[0071] Steps (1)-(5) are the same as in Example 5;

[0072] Step (6) is to co-culture the conjugated oligomer / gold nanocluster heterojunction with cyanobacteria in deionized water at a mass ratio of 1:50 for 7 days. The photocurrent of the cyanobacteria reaches 20 nA / cm 2 , improving the light energy conversion efficiency of the cyanobacteria. The lipid content of the cyanobacteria increases by 11.81%, promoting the increase of cyanobacterial biomass production.

[0073] The above is only an embodiment of the present application and is not intended to limit the present application. The present application can have various modifications and changes for those skilled in the art. Any modification, equivalent replacement, improvement, etc. within the spirit and principles of the present application shall be included in the scope of the claims of the present application.

Claims

1. A method for preparing a conjugated oligomer / gold nanocluster light energy conversion material, characterized in that, The specific steps are as follows: (1) Synthesis of main chain is fluorene and thiophene, side chain is conjugated oligomer material containing active group R, take conjugated oligomer and dissolve in deionized water according to 1:10000-1:1000 mass ratio, stir uniformly and ultrasonic dispersion, get conjugated oligomer solution; (2) The four hydroxymethyl phosphorus chloride solution, chloroauric acid solution and sodium hydroxide solution are mixed and stirred according to the volume ratio of 180:110:8000-180:150:8000, the pH of sodium hydroxide solution is 8-11, then the above solution is mixed and stirred with mercapto ligand solution according to the volume ratio of 1:100-1:50, 2-6℃ static 24-48 hours, prepare gold nanoparticles solution; (3) The gold nanoparticles solution obtained in step (2) is mixed and stirred with sodium tetraborate solution and mercaptoundecanoic acid ethanol solution according to the volume ratio of 20:8:3-100:8:3, ultrafiltration in ultrafiltration tube, get gold nanocluster solution; (4) The gold nanocluster solution obtained in step (3) is mixed and stirred with 1-ethyl-(3-dimethylaminopropyl) carbonyl diimide solution and N-hydroxyl thiosuccinimide solution according to the volume ratio of 100:50:50-100:1:1; (5) The solution obtained in step (4) is ultrafiltrated in ultrafiltration tube, then mixed and stirred with oligomer solution obtained in step (1) according to the volume ratio of 1:1-10:1, ultrafiltration in ultrafiltration tube, get conjugated oligomer / gold nanocluster light energy conversion material.

2. The production method according to claim 1, characterized by, The main chain in step (1) is one of the main chain structures of formula ①-④, wherein the number of m is 0-10, the number of n is 0-10, 。 3. The preparation method according to claim 1, characterized in that, The active group R in step (1) is one or both of carboxyl and amino.

4. The production method according to claim 1, characterized by, The mercapto ligand in step (2) is one or several of mercaptobenzoic acid, mercaptopropionic acid, mercaptohexanoic acid, disulfide ligand, mercaptoethylene glycol, thiophene derivative, bis-mercaptoethane, polythiol compound, mercaptoethanol, mercapto-p-toluenesulfonic acid, mercapto triethylene tetramine and glutathione.

5. The method of claim 1, wherein, The four hydroxymethyl phosphorus chloride solution in step (2) has a mass fraction of 0.5-5%, the chloroauric acid concentration is 10-200 mM, and the mercapto ligand solution concentration is 0.5-20 mM.

6. The method of claim 1, wherein, The sodium tetraborate solution concentration in step (3) is 1-25 mM, and the mercaptoundecanoic acid ethanol solution concentration is 0.01-0.2 mM.

7. The preparation method according to claim 1, characterized in that, The 1-ethyl-(3-dimethylaminopropyl) carbonyl diimide solution concentration in step (4) is 0.01-0.1 mM, and the N-hydroxyl thiosuccinimide solution concentration is 0.02-0.08 mM.

8. The method of claim 1, wherein, The ultrafiltration tube in steps (3) and (5) has a molecular weight cut-off of 300-10000, the ultrafiltration time is 0.1-0.5 hours, and the ultrafiltration centrifugal force is 2000-6000 g.

9. The method of claim 1, wherein, The mixing and stirring time of step (2) is 10-30 minutes, the mixing and stirring time of step (3) is 12-48 hours; the mixing and stirring time of step (4) is 10-30 minutes; the mixing and stirring time of step (5) is 0.5-24 hours.

10. Use of a conjugated oligomer / gold nanocluster light energy conversion material obtained by the production method according to any one of claims 1 to 8, characterized in that, The conjugated oligomer / gold nanocluster photoenergy conversion material is co-cultured with the photosynthetic organism in a good solvent according to a mass ratio of 1:400-1:

10.

11. Use of a conjugated oligomer / gold nanocluster light energy conversion material according to claim 10, characterized in that, The photosynthetic organism is one or more of cyanobacteria, red algae, cryptomonads, dinoflagellates, chrysophytes, yellow-green algae, diatoms, brown algae, euglenoids, green algae, desmids, photosynthetic bacteria, and higher plants.

12. Use of a conjugated oligomer / gold nanocluster light energy conversion material according to claim 10, characterized in that, The good solvent is one or more of deionized water, phosphate buffer, Tris buffer, agarose suspension, M9 buffer, LB buffer, physiological saline, BG11 medium, F / 2 medium, Walne medium, Provasoli medium, ASM-1 medium, Z8 medium, K medium, MS medium, B5 medium, White's medium, SH medium, WPM medium, NN medium, and Anderson's medium.

13. Use of a conjugated oligomer / gold nanocluster light energy conversion material according to claim 10, characterized in that, The co-culturing in the good solvent lasts for 0.5-7 days.

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