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Manpower method for cultivating quickly nostoc sphaeroids kutz algae

A fast technology, applied in the field of artificial cultivation of cyanobacterial algae, can solve the problems of long cycle, difficult to meet large-scale artificial cultivation, low seed production efficiency, etc. Simple to use effects

Inactive Publication Date: 2007-09-19
SHANGHAI NORMAL UNIVERSITY
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  • Description
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AI Technical Summary

Problems solved by technology

In summary, the existing technology of artificially cultivating Gexianmi algae has defects such as long cycle, low seed production efficiency, and difficulty in meeting the needs of large-scale artificial cultivation of Gexianmi.

Method used

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Embodiment Construction

[0009] The method for the artificial rapid cultivation of S. kudzui species provided by the present invention comprises the following specific steps:

[0010] 1. Prepare suitable culture medium

[0011] Weighing: sodium carbonate 25-45mg / L, dipotassium hydrogen phosphate 70-90mg / L, citric acid 4-7mg / L, ferric ammonium citrate 4-7mg / L, edetate disodium salt 0.5-2mg / L, calcium chloride 180-240mg / L, magnesium sulfate 60-90mg / L, boric acid 1.5-3g / L, manganese chloride 1.5-2g / L, zinc sulfate 0.2-0.3g / L, sodium molybdate 0.3- 0.4g / L, copper sulfate 0.05-0.1g / L, cobalt nitrate 0.03-0.06g / L, distilled to volume with sterile distilled water.

[0012] 2. Original species screening

[0013] Choose plump, dark green, and vigorously growing Gexianmi algae as the original species.

[0014] 3. Stock disinfection

[0015] Rinse the algae body of Gexianmi with sterile distilled water, then disinfect with 70% ethanol solution for 20-30 seconds, and then rinse with sterile distilled water f...

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Abstract

The present invention discloses a method for quick and artificial cultivation of Nostoc sphaeroids Kutz which comprise: preparing suitable cultivation liquid, screening original stock seed, disinfecting original stock seed, liquid inoculation and culture, algae germplasm formation and purification, amplified cultivation. In the present method of artificial cultivation of Nostoc sphaeroids Kutz , direct liquid inoculation and culture can be employed through optimum culture liquid without need of antibiotic sterilization and purification or solid culture medium purification process, which has short cultivation cycle and yield germplasm in large volume and operate easily compared to the existing technology, which fit the need of cultivating Nostoc sphaeroids Kutz in large scale.

Description

technical field [0001] The present invention relates to the artificial culture method of cyanobacterium algae species, more specifically, relates to the artificial culture method of Gexiana species. Background technique [0002] Gexianmi is a multicellular filamentous cyanobacteria. According to "Compendium of Materia Medica" and "Compendium of Materia Medica Supplements", Gexianmi is not only edible, but also has multiple curative effects, such as treating night blindness, rectal prolapse, burns and both The effect of beauty. However, the wild resources of Gexianmi are very scarce, and the ecological environment of Gexianmi has been destroyed. In order to meet market demand, it is urgent to be able to carry out artificial large-scale production. At present, there are existing research reports on the artificial cultivation method of the sarcocarpus species: the method disclosed in the Chinese patent document CN1294190 (application date: 1999.10.22) includes culture solution...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G1/00A01G31/00
Inventor 曹建国刘晶晶王全喜
Owner SHANGHAI NORMAL UNIVERSITY
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