Manpower method for cultivating quickly nostoc sphaeroids kutz algae
A fast technology, applied in the field of artificial cultivation of cyanobacterial algae, can solve the problems of long cycle, difficult to meet large-scale artificial cultivation, low seed production efficiency, etc. Simple to use effects
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[0009] The method for artificially and rapidly cultivating Pueraria lobata seeds provided by the present invention includes the following specific steps:
[0010] 1. Prepare the appropriate culture medium
[0011] Weigh: sodium carbonate 25-45mg / L, dipotassium hydrogen phosphate 70-90mg / L, citric acid 4-7mg / L, ferric ammonium citrate 4-7mg / L, ethylenediaminetetraacetic acid disodium salt 0.5-2mg / L, calcium chloride 180-240mg / L, magnesium sulfate 60-90mg / L, boric acid 1.5-3g / L, manganese chloride 1.5-2g / L, zinc sulfate 0.2-0.3g / L, sodium molybdate 0.3- 0.4g / L, copper sulfate 0.05-0.1g / L, cobalt nitrate 0.03-0.06g / L, make the volume constant with sterile distilled water.
[0012] 2. Original species selection
[0013] Choose the plump, dark green, and vigorous growth of Gexian Mi algae as the original species.
[0014] 3. Original seed disinfection
[0015] Rinse the body of Kudzu with sterile distilled water and disinfect with 70% ethanol solution for 20-30 seconds, and then rinse...
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