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Porous bacterial cellulose skin repair material with density structure and preparation method thereof

A technology of bacterial cellulose and bacterial cellulose membrane, which is applied in the field of porous bacterial cellulose skin repair material with a sparse and dense structure and its preparation, can solve the problem of high preparation cost, prolonging the treatment period and affecting the connectivity between the epidermis and the dermis. and other problems, to shorten the wound healing cycle, reduce the proliferation of scars, and achieve good structural continuity.

Inactive Publication Date: 2013-03-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such a structure not only slows down the growth of skin tissue cells at the interface between the dense and sparse layers of the material, but also destroys the continuity of the structure of the dermis and epidermis of the new skin, thus prolonging the treatment cycle and causing obvious scars to form on the wound
In addition, the preparation process of adhesive and coating film will greatly affect the connectivity between the epidermis and dermis, and ultimately affect the breathability of artificial skin materials
The dermis layer material of the artificial skin involved in the prior art usually chooses collagen and fibroin with good biocompatibility, and the epidermis material usually chooses degradable materials (polylactic acid, polyethylene glycol) or inert materials (silicone rubber, Polyvinylidene fluoride, polyurethane), which makes the preparation cost very high. At the same time, the epidermis and dermis are composed of two different materials, which also increases the difficulty of artificial compounding to ensure that the two materials are tightly combined.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1) Preparation of fermentation medium;

[0062] Components of the culture solution, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01;

[0063] The pH of the culture solution is 4.0;

[0064] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0065] 2) Bacteria expansion;

[0066] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 5 individual / mL.

[0067] 3) Static cultivation;

[0068] Transfer the expanded bacterial solution to a culture container filled with fermentation broth, place it in a constant temperature incubator, and culture it statically at 28°C;

[0069] The double-layer structure of the ...

Embodiment 2

[0079] 1) Preparation of fermentation medium;

[0080] Components of the culture solution, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 5, peptone 0.5, yeast extract 0.5, citric acid 0.1, disodium hydrogen phosphate 0.2, potassium dihydrogen phosphate 0.1;

[0081] The pH of the culture solution is 6.0;

[0082] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0083] 2) Bacteria expansion;

[0084] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 individual / mL.

[0085] 3) Static cultivation;

[0086] Transfer the expanded bacterial solution to a culture container filled with fermentation broth, place it in a constant temperature incubator, and culture it statically at 32°C;

[0087] The double-layer structure of the bacte...

Embodiment 3

[0097] 1) Preparation of fermentation medium;

[0098] Components of the fermentation broth, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 3, peptone 0.3, yeast extract 0.3, citric acid 0.05, disodium hydrogen phosphate 0.1, potassium dihydrogen phosphate 0.05;

[0099] The pH of the fermentation broth is 5.0;

[0100] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0101] 2) Bacteria expansion;

[0102] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 individual / mL.

[0103] 3) Static cultivation;

[0104] Transfer the expanded bacterial solution to a culture container filled with fermentation broth, place it in a constant temperature incubator, and culture it statically at 30°C;

[0105] The double-layer structure of the b...

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Abstract

The invention relates to a porous bacterial cellulose skin repair material with a density structure and a preparation method thereof. The porous bacterial cellulose skin repair material with the 'upper dense and lower loose' structure similar to human skin is prepared by controlling the culture condition of bacterial cellulose and adding sustained-release microspheres in the culture process. A loose layer is tightly combined with a dense layer, so that obvious physical layering is avoided, and the structural continuity is good; and gradient changes of the structures are produced in the loose layer and the dense layer, and multiple pores distributed uniformly are formed in the loose layer, so that the upper dense and lower loose gradient structure of the human skin is simulated to the utmost degree. Cells easily enter the material, the healing period of a wound is obviously shortened, and proliferation of healed scars is effectively reduced; and good air permeability and water holding property of the bacterial cellulose are kept, the wound surface can be kept in a wet environment, and healing of the wound surface is facilitated. The forming process is simple, the culture period is short, the preparation process is environment-friendly, simple, convenient and quick, and the preparation cost is low.

Description

technical field [0001] The invention relates to a porous bacterial cellulose skin repair material and a preparation method thereof, in particular to a porous bacterial cellulose skin repair material with a density structure and a preparation method thereof, in particular to a kind of porous bacterial cellulose skin repair material by adjusting the fermentation culture liquid The air pressure and oxygen concentration above are used to realize the dense and sparse double-layer structure of the bacterial cellulose film, and the slow-release microspheres are added during the cultivation process to obtain a porous bacterial cellulose skin repair material with a dense and dense structure. Background technique [0002] The skin is covered on the surface of the whole body of the human body and is in direct contact with the external environment. It is an important boundary organ in anatomy and physiology. The skin not only protects the body from pollutants or bacteria, but also keeps...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/56A61L27/40C12P19/04C12R1/02C12R1/01C12R1/38C12R1/41
Inventor 李喆杨敬轩王利群郑羿张云王华平陈仕艳
Owner DONGHUA UNIV
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