Bacterial cellulose skin regeneration material with density structure and preparation method thereof

A technology of bacterial cellulose and bacterial cellulose film, which is applied in the field of bacterial cellulose skin repair materials with dense structure and its preparation, can solve the problems of high preparation cost, prolonging the treatment period, wound scars, etc., and achieve good air permeability The effect of water holding capacity, reduction of scar hyperplasia, and good biocompatibility

Inactive Publication Date: 2015-04-01
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such a structure not only slows down the growth of skin tissue cells at the interface between the dense and sparse layers of the material, but also destroys the continuity of the structure of the dermis and epidermis of the new skin, thus prolonging the treatment cycle and causing obvious scars to form on the wound
In addition, the preparation process of adhesive and coating film will greatly affect the connectivity between the epidermis and dermis, and ultimately affect the breathability of artificial skin materials
The dermis layer material of the artificial skin involved in the prior art usually chooses collagen and fibroin with good biocompatibility, and the epidermis material usually chooses degradable materials (polylactic acid, polyethylene glycol) or inert materials (silicone rubber, Polyvinylidene fluoride, polyurethane), which makes the preparation cost very high. At the same time, the epidermis and dermis are composed of two different materials, which also increases the difficulty of artificial compounding to ensure that the two materials are tightly combined.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 1) Preparation of fermentation medium;

[0061] Components of the fermentation medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, and amount of water;

[0062] The pH of the fermentation broth is 4.0;

[0063] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0064] 2) Bacteria expansion;

[0065] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 5 individual / mL.

[0066] 3) Static cultivation;

[0067] Transfer the expanded bacterial solution to a culture container filled with fermentation broth, place it in a constant temperature incubator, and culture it statically at 28°C;

[0068] By adju...

Embodiment 2

[0078] 1) Preparation of fermentation medium;

[0079] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and amount of water;

[0080] The pH of the fermentation broth is 6.0;

[0081] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0082] 2) Bacteria expansion;

[0083] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 individual / mL.

[0084] 3) Static cultivation;

[0085] Transfer the expanded bacterial solution to a culture container filled with fermentation broth, place it in a constant temperature incubator, and culture it statically at 32°C;

[0086] By adjusting...

Embodiment 3

[0096] 1) Preparation of fermentation medium;

[0097] Components of the fermentation medium, in mass percent, in wt%: 3 glucose, fructose, sucrose or mannitol, 0.3 peptone, 0.3 yeast extract, 0.05 citric acid, 0.1 disodium hydrogen phosphate, 0.05 potassium dihydrogen phosphate, and amount of water;

[0098] The pH of the fermentation broth is 5.0;

[0099] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0100] 2) Bacteria expansion;

[0101] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 individual / mL.

[0102] 3) Static cultivation;

[0103] Transfer the expanded bacterial solution to a culture container filled with fermentation broth, place it in a constant temperature incubator, and culture it statically at 30°C;

[0104] By adjusti...

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Abstract

The invention relates to bacterial cellulose skin regeneration material with a density structure and a preparation method thereof. A 'thick-end-up' biological structure which is similar to skin of a human body is prepared by controlling of a culture condition of bacterial cellulose, and artificial skin is good in biocompatibility and cell viability. The combination degree of the sparse layer and a compact layer is deep and is not only limited to the material surface. Obvious physical layering does not exist, structural continuity is good, structural gradient change exists in the sparse layer and the compact layer, and a thick-end-up gradual change gradient structure imitates the skin of the human body to the maximum extent. The bacterial cellulose skin regeneration material with the density structure can obviously shorten wound healing period, effectively reduces cicatricial hyperplasia after a wound is healed, keeps good air permeability and water binding capacity of bacterial cellulose, can enable the wound to maintain a hygroscopic state environment for a long time, and is beneficial to healing the wound. Forming process is simple, culture period is short, preparation process is green and environmental-friendly, simple and quick, and preparation cost is low.

Description

technical field [0001] The present invention relates to a bacterial cellulose skin repair material with a density structure and a preparation method thereof, in particular to a bacterial cellulose skin repair material with a gradient density structure and a preparation method thereof, specifically a bacterial cellulose skin repair material with a gradient density structure and a preparation method thereof. Adjusting the overall pressure of the air above the culture solution and the partial pressure of oxygen to realize the double-layer structure of the bacterial cellulose film with a dense top and a sparse bottom to obtain a bacterial cellulose skin repair material and a preparation method thereof. Background technique [0002] The skin is covered on the surface of the whole body of the human body and is in direct contact with the external environment. It is an important boundary organ in anatomy and physiology. The skin not only protects the body from pollutants or bacteria...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/10A61L27/20A61L27/60
Inventor 杨敬轩李喆张云王利群王华平陈仕艳洪枫
Owner DONGHUA UNIV
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