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Hollow heterotype bacteria cellulose artificial blood vessel stent material with gradient structure and preparation method thereof

A technology of bacterial cellulose and scaffold materials, applied in medical science, prosthesis, fermentation, etc., can solve the problem that tissue engineering scaffold materials cannot meet the requirements of qualified artificial blood vessel materials

Inactive Publication Date: 2013-04-03
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the embodiment described in CN101584882A is not designed closely around the requirements of artificial blood vessels, and the obtained tissue engineering scaffold material based on bacterial cellulose cannot meet the requirements of qualified artificial blood vessel materials in theory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 1) Preparation of fermentation medium;

[0064] Components of the fermentation medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, and amount of water;

[0065] The pH of the fermentation broth is 4.0;

[0066] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0067] 2) Bacteria expansion;

[0068] The fermentation culture liquid is inserted into the bacterial liquid and expanded; the degree of expansion: the number of strain cells is 2×10 5 pieces / ml.

[0069] 3) Cultivation stage;

[0070] Transfer the expanded bacterial solution to a culture container filled with fermentation medium, place it in a constant temperature incubator, and cultivate it at 28°C;

[00...

Embodiment 2

[0084] 1) Preparation of fermentation medium;

[0085] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and amount of water;

[0086] The pH of the fermentation broth is 6.0;

[0087] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0088] 2) Bacteria expansion;

[0089] The fermentation culture liquid is inserted into the bacterial liquid and expanded; the degree of expansion: the number of strain cells is 2×10 7 pieces / ml.

[0090] 3) Cultivation stage;

[0091] Transfer the expanded bacterial solution to a culture container filled with fermentation medium, place it in a constant temperature incubator, and cultivate it at 32°C;

[0092] B...

Embodiment 3

[0105] 1) Preparation of fermentation medium;

[0106] Components of the fermentation medium, in mass percent, in wt%: 3 glucose, fructose, sucrose or mannitol, 0.3 peptone, 0.3 yeast extract, 0.05 citric acid, 0.1 disodium hydrogen phosphate, 0.05 potassium dihydrogen phosphate, and amount of water;

[0107] The pH of the fermentation broth is 5.0;

[0108] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0109] 2) Bacteria expansion;

[0110] The fermentation culture liquid is inserted into the bacterial liquid and expanded; the degree of expansion: the number of strain cells is 2×10 7 pieces / ml.

[0111] 3) Cultivation stage;

[0112] Transfer the expanded bacterial solution to a culture container filled with fermentation medium, place it in a constant temperature incubator, and cultivate it at 30°C;

[0113]...

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Abstract

The invention relates to a hollow heterotype bacteria cellulose artificial blood vessel stent material with a gradient structure and a preparation method thereof. The artificial blood vessel stent with a 'compact inner cavity, elastic inside, loose outer wall' biological structure similar to a human blood vessel tissue can be prepared by controlling the culture condition of bacteria cellulose, and has excellent biocompatibility and cell viability, wherein the combination between a compact layer and an elastic layer and between the elastic layer and a loose layer can be in a further degree, and not limited to the surface of the material; physical layering is not remarkable, the structure continuity is excellent, and the 'compact inside and loose outside' in the human blood vessel tissue can be furthest imitated due to the gradient changing structure in the compact layer, the elastic layer and the loose layer so that the time for culturing cells in vitro can be shortened, the rejecting phenomenon of a human body to a foreign transplant can be remarkably reduced, the transplant success rate can be greatly improved, the stent forming process is simple, the culture period is short, preparation process is green and environment-friendly, easy and quick, and the preparation cost is low.

Description

technical field [0001] The invention relates to a hollow special-shaped bacterial cellulose artificial vascular scaffold material with a gradient structure and a preparation method thereof, in particular to a method for realizing the automatic growth of the bacterial cellulose material by adjusting the overall air pressure and oxygen partial pressure inside the oxygen-permeable mold. A hollow special-shaped bacterial cellulose artificial vascular stent material prepared from a gradient structure gradually changing from dense to loose from inside to outside and a preparation method thereof. Background technique [0002] The human blood circulation system consists of three parts: blood, blood vessels and heart. Blood vessels serve as bridges to connect various organs and hearts in series, provide channels for blood flow, and transport nutrients and nutrients in the blood to various parts of the body. Therefore, often It will also be called the cardiovascular system, and the le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20C12P19/04
Inventor 杨敬轩李喆郑羿王利群王华平陈仕艳张云
Owner DONGHUA UNIV
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