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Bacteria cellulose controlled-release carrier material of sandwich structure and preparation method thereof

A technology of bacterial cellulose and bacterial cellulose membrane, which is applied in the field of bacterial cellulose slow-release carrier materials and its preparation, can solve the problems of waste of resources, environmental pollution, etc., increase the area of ​​aerobic area, and solve the problem of changing the growth rate of cellulose The effect of slow and good controlled release performance and drug loading performance

Inactive Publication Date: 2013-03-27
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the use of a large number of fine chemical products, problems such as environmental pollution and waste of resources have been brought

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1) Preparation of fermentation medium;

[0058] Components of the fermentation medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, and amount of water;

[0059] The pH of the fermentation broth is 4.0;

[0060] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0061] 2) Bacteria expansion;

[0062] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 5 individual / mL.

[0063] 3) Static cultivation;

[0064] Transfer the expanded bacterial solution to a culture container filled with fermentation broth, place it in a constant temperature incubator, and culture it statically at 28°C;

[0065] By adju...

Embodiment 2

[0076] 1) Preparation of fermentation medium;

[0077] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and amount of water;

[0078] The pH of the fermentation broth is 6.0;

[0079] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0080] 2) Bacteria expansion;

[0081] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 individual / mL.

[0082] 3) Static cultivation;

[0083] Transfer the expanded bacterial solution to a culture container filled with fermentation broth, place it in a constant temperature incubator, and culture it statically at 32°C;

[0084] By adjusting...

Embodiment 3

[0095] 1) Preparation of fermentation medium;

[0096] Components of the fermentation medium, in mass percent, in wt%: 3 glucose, fructose, sucrose or mannitol, 0.3 peptone, 0.3 yeast extract, 0.05 citric acid, 0.1 disodium hydrogen phosphate, 0.05 potassium dihydrogen phosphate, and amount of water;

[0097] The pH of the fermentation broth is 5.0;

[0098] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0099] 2) Bacteria expansion;

[0100] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 individual / mL.

[0101] 3) Static cultivation;

[0102] Transfer the expanded bacterial solution to a culture container filled with fermentation broth, place it in a constant temperature incubator, and culture it statically at 30°C;

[0103] By adjusti...

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Abstract

The invention relates to a bacteria cellulose controlled-release carrier material of a sandwich structure and a preparation method thereof. The bacteria cellulose controlled-release carrier material of the sandwich structure is manufactured through steps such as enlarging and cultivating culture, static cultivate and post treatment, the bacteria cellulose controlled-release carrier material is formed by three-layer of well-knit bacteria cellulose films, and the three-layer of tight combining bacteria cellulose films comprise an upper controlled-release layer, a low controlled-release layer and a control release layer positioned on the middle; the tight combination means that cellulose microfibril of the controlled-release layer and cellulose microfibril of the control release layer are combined with molecules in an intermolecular hydrogen through a beta-1,4 glucose chain, the obvious physical layering is not generated, and the structural continuity is good; meanwhile, the good control release performance and medicine carrying performance of the bacteria cellulose are kept, and the preparation process is environmentally-friendly, simple, convenient and rapid; and the preparation cost is cheap, and the bacteria cellulose controlled-release carrier material is widely applied to fields of medicines, foods, agriculture, petrochemical industry and daily service chemical industry.

Description

technical field [0001] The invention relates to a slow-release carrier material and a preparation method thereof, in particular to a bacterial cellulose slow-release carrier material with a sandwich structure and a preparation method thereof. Background technique [0002] With the development of science and technology and the progress of society, all kinds of fine chemical products have become an indispensable part of the daily production and life of human society. For example, slow-release sterilization, insecticide, and anti-mildew products used daily are important tools to ensure people's hygiene and cleanliness; pesticides and fertilizers used in agricultural production to prevent and control crop diseases, insects, and increase production are indispensable for modern agriculture. Fewer means of production; drugs used in biomedicine are necessary weapons to protect human health and alleviate diseases; active ingredients and essential oils used in beauty cosmetics are eff...

Claims

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Application Information

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IPC IPC(8): B32B23/00C12P19/04
Inventor 李喆王利群杨敬轩郑羿王华平陈仕艳洪枫
Owner DONGHUA UNIV
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