Method for increasing biomass and astaxanthin content of haematococcus pluvialis

A technology of Haematococcus pluvialis and biomass, applied in the field of bioengineering, can solve the problems of difficult operation, complicated technical process, not very significant, etc., achieves simple and easy operation, short cultivation period, improved biomass and astaxanthin The effect of nutrient content

Inactive Publication Date: 2018-11-06
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Although the biomass of Haematococcus pluvialis and the accumulation of astaxanthin in the above-mentioned related patents have increased, it is not very significant or the technical process is relatively complicated and difficult to operate

Method used

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  • Method for increasing biomass and astaxanthin content of haematococcus pluvialis
  • Method for increasing biomass and astaxanthin content of haematococcus pluvialis
  • Method for increasing biomass and astaxanthin content of haematococcus pluvialis

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preparation example Construction

[0019] (1) Preparation and sterilization of the culture medium: prepare a nitrogen-sufficient BBM medium, prepare a nitrogen-sufficient BBM medium, add sodium acetate or magnesium acetate to it, and sterilize the medium by high-pressure moist heat (sterilization conditions are 121°C, 20min;); the amount of sodium acetate added is 0.5~2g / L, and the amount of magnesium acetate added is 0.05~0.5 g / L;

[0020] (2) Preparation of algae liquid: Haematococcus pluvialis seed liquid is cultivated at 24-26°C, light intensity 2000-2500lx, and continuous light conditions, cultivated to the late logarithmic growth phase, centrifuged, and prepared in step (1) Dilute the seed solution of Haematococcus pluvialis to 2.5×10 5 cells / mL is used as the test algae liquid; the test algae liquid is routinely cultured at a temperature of 24-26°C and a light intensity of 2000-2500lx, and is replenished with sterile water every other day, and samples are taken to measure its biomass; The measurement me...

Embodiment 1

[0028] (1) Sodium acetate was added to nitrogen-enriched BBM medium to make the concentrations 0 g / L, 0.5 g / L, 1 g / L, and 2 g / L, respectively.

[0029] (2) Haematococcus pluvialis strains are used Haematococcus pluvialis LUGU, use BBM medium, culture at temperature (any temperature between 24-26°C), light intensity (any value between 2000-2500lx), and continuous light conditions, and cultivate Haematococcus pluvialis to reach logarithm During the growth period, use the BBM medium prepared in (1) to dilute to 2.5×10 5 cells / mL is used as the test algae liquid; then the above-mentioned algae liquid is routinely cultured under the conditions of (any temperature between 24-26°C) and light intensity (any value between 2000-2500lx); regular sampling every other day, Then centrifuge and lyophilize, and weigh the dry weight of the cells.

[0030] It was found that on the 5th day, when the concentration of sodium acetate was added to 2 g / L, the dry weight of the cells reached the ma...

Embodiment 2

[0040] (1) Magnesium acetate was added to nitrogen-enriched BBM medium to make the concentrations 0, 0.05, 0.1, and 0.5 g / L, respectively.

[0041] (2) Haematococcus pluvialis strains are used Haematococcus pluvialis LUGU , use BBM medium, culture at temperature (any temperature between 24-26°C), light intensity (any value between 2000-2500lx), and continuous light conditions, and cultivate Haematococcus pluvialis to reach logarithm During the growth period, use the BBM medium prepared in (1) to dilute to 2.5×10 5 cells / mL was used as the test algae liquid. Then the above algae liquid is routinely cultured under the conditions of (any temperature between 24-26°C) and light intensity (any value between 2000-2500lx). Samples were taken regularly every other day, then centrifuged and freeze-dried, and the dry weight of cells was weighed.

[0042] It was found that on the 7th day, when the concentration of magnesium acetate was added to be 0.5g / L, the dry weight of the cells ...

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Abstract

The invention relates to a method for increasing biomass and astaxanthin content of haematococcus pluvialis, and belongs to the technical field of bioengineering. The method comprises steps as follows: a BBM culture medium with sodium acetate or magnesium acetate added is prepared firstly, after being sterilized, the BBM culture medium is inoculated with the haematococcus pluvialis cultured to theexponential phase until the concentration reaches 2.0*10<5>-2.5*10<5> cells / mL, culturing is performed at the temperature of 24-26 DEG C under the condition of light intensity being 2000-2500 lx, andsampling is performed every other day for biomass measurement; algae cells are collected when the biomass is maximum, diluted by the nitrogen-deficient BBM culture medium with melatonin added until the concentration is 2.0*10<5>-2.5*10<5> cells / mL, culturing is performed at the temperature of 26-28 DEG C under the condition of light intensity being 12000-14000 lx, and sampling is performed everyother day for astaxanthin content measurement. The method is simple and easy to operate, growth cycle of the haematococcus pluvialis can be shortened to a certain degree, and the biomass and the astaxanthin content of the haematococcus pluvialis are increased.

Description

technical field [0001] The invention relates to a method for increasing the biomass and astaxanthin content of Haematococcus pluvialis, belonging to the technical field of bioengineering. Background technique [0002] Natural astaxanthin is the strongest antioxidant found in nature so far. Its antioxidant activity far exceeds the existing antioxidants, and it is known as a "super oxidant". Because natural astaxanthin has good biological activity and biological safety, it has broad market prospects in industries such as food, pharmaceutical cosmetics, health care products, soft drink processing and aquatic products, poultry and livestock (chicken, pig and cattle) feed additives. [0003] At present, the main production methods of astaxanthin include artificial synthesis and biological acquisition. Synthetic astaxanthin is not only expensive, but also significantly different from natural astaxanthin in terms of structure, function, application, stability, absorption effect an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P23/00C12R1/89
CPCC12N1/12C12P23/00
Inventor 余旭亚崔静丁巍
Owner KUNMING UNIV OF SCI & TECH
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