Method for cultivating tissues to obtain seedlings of dendrobium huoshanense at one step and formula of nutrient liquid for method

A technology of Dendrobium huoshanense and tissue culture, which is applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of low seedling resistance, high cost and long time of group culturing seedlings, etc., and increase the survival rate. and germination rate, shorten the cultivation period, and reduce the effect of planting input

Active Publication Date: 2015-12-02
戴亚峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing group seedling cultivation technology of Dendrobium Huoshanense has high cost (requiring a large amount of labor cost), long time (it takes at least 12 months from seed to seedling), and high mutation rate (due to the value-added and hormone induction, There are many variations), and the cultivation survival rate is low (continuous value-added makes the resistance of the seedlings lower, and the seedlings must be hardened before they can be cultivated, and the survival rate is only about 80%)

Method used

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  • Method for cultivating tissues to obtain seedlings of dendrobium huoshanense at one step and formula of nutrient liquid for method
  • Method for cultivating tissues to obtain seedlings of dendrobium huoshanense at one step and formula of nutrient liquid for method
  • Method for cultivating tissues to obtain seedlings of dendrobium huoshanense at one step and formula of nutrient liquid for method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] A method for growing seedlings of Dendrobium huoshanense tissue culture in one step, comprising the following steps:

[0085] Step 1: the preparation of solid medium, wherein the formula of solid nutrient base comprises:

[0086] The following composition and ratio of a large number of elements:

[0087] Potassium nitrate KNO 3 : 400mg / L;

[0088] Ammonium sulfate (NH4)2SO4: 500mg / L;

[0089] Potassium dihydrogen phosphate (KH2PO4): 400mg / L;

[0090] Magnesium sulfate MgSO4.7H2O: 250mg / L;

[0091] Calcium nitrate Ca(NO4)2: 450mg / L;

[0092] The following composition and proportion of trace elements:

[0093] Potassium iodide KI: 0.83mg / L;

[0094] Boric acid H3BO3: 6.2mg / L;

[0095] Manganese sulfate MnSO4.4H2O: 22.3mg / L;

[0096] Zinc sulfate ZnSO4.7H2O: 8.6mg / L;

[0097] Sodium molybdate Na2MoO4.2H2O: 0.25mg / L;

[0098] Copper sulfate CuSO4.5H2O: 0.025mg / L;

[0099] Cobalt chloride CoCl2.62: 0.025mg / L;

[0100] The iron salt of following composition and p...

Embodiment 2

[0126] A method for one-step seedling growth of Dendrobium huoshanense tissue culture, comprising preparation of solid medium, preparation of liquid nutrient solution, selection and disinfection of dendrobium huoshanense fruit pods, preparation of dendrobium huoshanense seed suspension, aseptic sowing, seedling cultivation and Emergence standards, the specific steps are as follows:

[0127] Step 1: the preparation of solid medium, wherein the formula of solid nutrient base comprises:

[0128] The following composition and ratio of a large number of elements:

[0129] Potassium nitrate KNO 3 : 300mg / L;

[0130] Ammonium sulfate (NH4)2SO4: 400mg / L;

[0131] Potassium dihydrogen phosphate (KH2PO4): 300mg / L;

[0132] Magnesium sulfate MgSO4.7H2O: 200mg / L;

[0133]Calcium nitrate Ca(NO4)2: 400mg / L;

[0134] The following composition and proportion of trace elements:

[0135] Potassium iodide KI: 0.65mg / L;

[0136] Boric acid H3BO3: 5.3mg / L;

[0137] Manganese sulfate MnSO4...

Embodiment 3

[0167] A method for one-step seedling growth of Dendrobium huoshanense tissue culture, comprising preparation of solid medium, preparation of liquid nutrient solution, selection and disinfection of dendrobium huoshanense fruit pods, preparation of dendrobium huoshanense seed suspension, aseptic sowing, seedling cultivation and Emergence standards, the specific steps are as follows:

[0168] Step 1: the preparation of solid medium, wherein the formula of solid nutrient base comprises:

[0169] The following composition and ratio of a large number of elements:

[0170] Potassium nitrate KNO 3 : 500mg / L;

[0171] Ammonium sulfate (NH4)2SO4: 600mg / L;

[0172] Potassium dihydrogen phosphate (KH2PO4): 500mg / L;

[0173] Magnesium sulfate MgSO4.7H2O: 300mg / L;

[0174] Calcium phosphate Ca(NO4)2: 500mg / L;

[0175] The following composition and proportion of trace elements:

[0176] Potassium iodide KI: 0.95mg / L;

[0177] Boric acid H3BO3: 8.9mg / L;

[0178] Manganese sulfate Mn...

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Abstract

The invention discloses a method for cultivating tissues to obtain seedlings of dendrobium huoshanense at one step. The method includes steps of preparing solid cultivation media; preparing liquid nutrient liquid according to a formula of the solid cultivation media; selecting and disinfecting dendrobium huoshanense capsules; preparing dendrobium huoshanense seed suspension liquid; sowing the dendrobium huoshanense seed suspension liquid in an aseptic condition; cultivating the seedlings; germinating the seedlings. The solid cultivation media are solid nutrient media, and the solid nutrient media in a formula comprise macroelements, trace elements, iron salt, organic components, hormone and additives. The liquid nutrient liquid is free of hormone, agar, chlormequat and banana and apple extracted liquid. The method for cultivating the dendrobium huoshanense has the advantages that the dendrobium huoshanense cultivation period can be shortened, the seedlings can be germinated in 6 months, in other words, the seedlings can be germinated in exactly seedling planting periods in April of a second year if seeds in October of a first year are sown, accordingly, the production cost for cultivating the seedlings of dendrobium huoshanense from the tissues can be reduced by 50%, the planting input can be reduced, and the cultivation survival rate and the cultivation germination rate can be increased.

Description

technical field [0001] The invention relates to the technical field of plant group cultivation seedlings, in particular to a method for tissue culture of Dendrobium huoshanense. Background technique [0002] Dendrobium huoshanense (scientific name: DendrobiumhuoshanenseC.Z.TangetS.J.Cheng), commonly known as rice dendrobium, is a herbaceous plant of the genus Dendrobium orchidaceae. Because it contains alkaloids, crude polysaccharides, amino acids, trace elements and other ingredients, it has the functions of strengthening kidney, clearing sound, improving eyesight, protecting liver and throat, nourishing yin and clearing heat, nourishing stomach and promoting body fluid, inhibiting tumor cell growth, and enhancing immunity. It is regarded as the first of the nine "immortal grasses". [0003] In the wild state, Dendrobium Huoshanense grows very slowly, which is extremely small, consisting of only a simple embryo and a single-layer wing-like seed coat, without endosperm. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 戴亚峰
Owner 戴亚峰
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