Method for efficiently breeding tissue culture seedlings of bletilla striata and planting method of bletilla striata
A bletilla striata group, high-efficiency technology, applied in the field of plant seedlings, can solve the problems of complex operation, cumbersome steps, increased pollution probability, etc., to achieve the effect of simplifying the operation steps, improving the survival rate and reducing the pollution rate
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Embodiment 1
[0034] A method for efficiently propagating bletilla striata tissue culture seedlings:
[0035] Preparation of improved MS medium: the formula is shown in Table 1, mixed and then autoclaved.
[0036] The improved MS culture medium of table 1 embodiment 1
[0037] name
Dosagemg / L
potassium nitrate
1900
ammonium nitrate
1650
Calcium Chloride Dihydrate
440
Magnesium Sulfate Heptahydrate
370
Potassium dihydrogen phosphate
170
potassium iodide
0.83
boric acid
6.2
Manganese Sulfate Tetrahydrate
22.3
Zinc sulfate heptahydrate
8.6
Sodium molybdate
0.25
Copper Sulfate Pentahydrate
0.025
Cobalt chloride
0.025
ferrous sulfate
27.8
[0038] Ethylenediaminetetraacetic acid
24.3
Inositol
100
Niacin
0.8
Pyridoxine Hydrochloride
0.8
Thiamine hydrochloride
0.8
Glycine ...
Embodiment 2
[0044] A method for efficiently propagating bletilla striata tissue culture seedlings:
[0045] Preparation of improved MS medium: the formula is shown in Table 2, mixed and then autoclaved.
[0046] The improved MS culture medium of table 2 embodiment 2
[0047] name
Dosagemg / L
potassium nitrate
1890
ammonium nitrate
1660
Calcium Chloride Dihydrate
445
Magnesium Sulfate Heptahydrate
375
[0048] Potassium dihydrogen phosphate
165
potassium iodide
0.75
boric acid
5
Manganese Sulfate Tetrahydrate
24
Zinc sulfate heptahydrate
10
Sodium molybdate
0.2
Copper Sulfate Pentahydrate
0.03
Cobalt chloride
0.03
ferrous sulfate
30
Ethylenediaminetetraacetic acid
26
Inositol
95
Niacin
1
Pyridoxine Hydrochloride
1
Thiamine hydrochloride
1
Glycine
4
pH
...
Embodiment 3
[0054] A method for efficiently propagating bletilla striata tissue culture seedlings:
[0055] Preparation of improved MS medium: the formula is shown in Table 3, mixed and then autoclaved.
[0056] The improved MS culture medium of table 3 embodiment 3
[0057] name
Dosagemg / L
potassium nitrate
1895
ammonium nitrate
1655
Calcium Chloride Dihydrate
442
Magnesium Sulfate Heptahydrate
373
Potassium dihydrogen phosphate
165
potassium iodide
0.8
boric acid
5.5
Manganese Sulfate Tetrahydrate
23.6
Zinc sulfate heptahydrate
10
Sodium molybdate
0.2
Copper Sulfate Pentahydrate
0.03
Cobalt chloride
0.028
ferrous sulfate
29
Ethylenediaminetetraacetic acid
25
Inositol
98
Niacin
0.9
Pyridoxine Hydrochloride
0.9
Thiamine hydrochloride
0.9
Glycine
3.5
pH
5.9
...
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