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Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application

A high-efficiency microorganism and wastewater treatment technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long treatment process, microbial decomposition and utilization, and inability to remove pollutants, and achieve a short culture and domestication period. , saving investment costs, the effect of the best treatment effect

Active Publication Date: 2010-12-01
北京三泰正方生物环境科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the continuous development of industry and the continuous use of new technologies and materials in recent decades, the pollutants in the environment have become more complex, especially a large number of artificially synthesized compounds entering the environment, such substances are mainly alkanes, Olefins, alicyclic hydrocarbons, aromatic hydrocarbons, synthetic organic substances, especially macromolecules, refractory, toxic and harmful substances such as phenols (phenol, chlorophenol, cresol, nitrophenol, etc.), aromatic hydrocarbons ( Benzene, toluene, xylene, nitrobenzene, phenols, naphthalene, anthracene, quinone, etc.), cyanides, amines, and ammonia nitrogen enter the environment with the wastewater or solid waste discharged from the factory. Due to the complex structure of these substances Due to the strangeness of nature and biology, they cannot be decomposed and utilized by microorganisms in a short period of time. The traditional wastewater treatment method using activated sludge to cultivate domesticated microorganisms can no longer effectively remove these pollutants. These substances have accumulated in the environment for a long time. The ecological environment on which we live has caused great pollution, which has brought great harm to human physical and mental health.
In addition, due to the continuous development of urban construction, urban land is in short supply year by year, while the traditional activated sludge treatment process is long, occupies a large area, and the contradictions such as complex operation are becoming increasingly prominent.

Method used

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  • Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application
  • Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application
  • Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Select Bacillus subtilis, Pseudomonas fluorescens, Alcaligenes faecalis, Aspergillus niger and Saccharomyces cerevisiae, and carry out step-by-step seed expansion cultivation of the above-mentioned strains respectively.

[0032]2. Add 5000mL of clean tap water to a container after disinfection, add glucose 200g, potassium nitrate 7.5g, ammonium nitrate 7.5g, calcium chloride 2.0g, magnesium sulfate 2.25g, potassium dihydrogen phosphate 750mg, inositol 750mg 125mg of ferrous sulfate, 100mg of manganese sulfate, 25mg of zinc sulfate, 25mg of boric acid, 2.5mg of potassium iodide, 1.0mg of sodium molybdate, 0.1mg of copper sulfate, fully stir to dissolve, mix well, and adjust the pH to 6.0. Take 5 more sterilized 2000mL beakers, and divide the above-mentioned solution equally among 5 beakers, each beaker is 1000mL.

[0033] 3. Inoculate 100mL of the seed solution of Bacillus subtilis, Pseudomonas fluorescens, Alcaligenes faecalis, Aspergillus niger and Saccharomyces cer...

Embodiment 2

[0036] 1. Select Bacillus sphaericus, Pseudomonas alcaligenes, Alcaligenes denitrificans, Aspergillus oryzae and Candida utilis, and carry out step-by-step seed expansion cultivation of the above-mentioned strains respectively.

[0037] 2. Add 2500mL of clean tap water to a sterilized container, add glucose 150g, potassium nitrate 6.25g, ammonium nitrate 5g, calcium chloride 1.25g, magnesium sulfate 1.25g, potassium dihydrogen phosphate 500mg, inositol 625mg , ferrous sulfate 87.5mg, manganese sulfate 62.5mg, zinc sulfate 25mg, boric acid 25mg, potassium iodide 2.5mg, sodium molybdate 0.75mg, copper sulfate 0.075mg, fully stir to dissolve, mix evenly, and adjust the pH to 8.0. Take 5 more sterilized 2000mL beakers, and divide the prepared solution into 5 beakers, each 500mL.

[0038] 3. Add 400 g of sterilized bran, 50 g of corn flour, and 50 g of bean cake powder to the beakers of the above 5 culture solutions, mix and stir evenly.

[0039] 4. Inoculate 50mL of the seed solu...

Embodiment 3

[0042] 1. Select Bacillus amyloliquefaciens and Clostridium saccharolyticus of the genus Bacillus, Pseudomonas aeruginosa and Pseudomonas denitrification of the genus Pseudomonas, Alcaligenes faecalis of the genus Alcaligenes and denitrification products For Alcalibacterium, Aspergillus niger and Aspergillus oryzae of the genus Aspergillus, and Saccharomyces cerevisiae and Candida utilis of the genus Saccharomyces, the above-mentioned bacterial species and each bacterial species were respectively subjected to step-by-step seed expansion cultivation.

[0043] 2. Add 2500mL of clean tap water to a container after disinfection, add glucose 125g, potassium nitrate 5g, ammonium nitrate 4.5g, calcium chloride 1.2g, magnesium sulfate 1.2g, potassium dihydrogen phosphate 450mg, inositol 500mg, 75mg of ferrous sulfate, 55mg of manganese sulfate, 20mg of zinc sulfate, 20mg of boric acid, 2mg of potassium iodide, 0.7mg of sodium molybdate, 0.07mg of copper sulfate, fully stir to dissolve,...

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Abstract

The invention discloses a composite efficient microbial preparation for treating difficultly-degradable waste water. The composite efficient microbial preparation comprises the following active ingredients of: bacillus, pseudomonas, alcaligenes eutrophus, aspergillus and yeast. The composite efficient microbial preparation has a unique treating effect on high-concentration organic sewage and highammonia and nitrogen sewage which are difficult to treat by the conventional activated sludge process and contain macromolecules and difficultly-degradable harmful constituents.

Description

technical field [0001] The invention relates to a composite high-efficiency microbial preparation for refractory wastewater treatment and its preparation and application. Background technique [0002] Biological wastewater treatment technology is the leading technology in the field of wastewater treatment. As an economical, reasonable and effective wastewater treatment technology, it plays an important role in improving the quality of water environment and preventing water pollution. [0003] The biological treatment of wastewater is to use the metabolism of microorganisms to decompose and transform pollutants in wastewater to achieve the purpose of purifying water quality. Its core is to cultivate and domesticate the dominant microbial flora suitable for the characteristics of wastewater, and maintain the microbial biomass and activity compatible with the concentration of wastewater pollutants, so that the dominant microbial flora can be treated at the best degradation acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/14C12N1/16C12N1/20C02F3/34C12R1/07C12R1/125C12R1/38C12R1/385C12R1/39C12R1/05C12R1/685C12R1/69C12R1/72C12R1/865
Inventor 李沧海付建军黄海魏雪松
Owner 北京三泰正方生物环境科技发展有限公司
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