Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application
A high-efficiency microorganism and wastewater treatment technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long treatment process, microbial decomposition and utilization, and inability to remove pollutants, and achieve a short culture and domestication period. , saving investment costs, the effect of the best treatment effect
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Embodiment 1
[0031] 1. Select Bacillus subtilis, Pseudomonas fluorescens, Alcaligenes faecalis, Aspergillus niger and Saccharomyces cerevisiae, and carry out step-by-step seed expansion cultivation of the above-mentioned strains respectively.
[0032]2. Add 5000mL of clean tap water to a container after disinfection, add glucose 200g, potassium nitrate 7.5g, ammonium nitrate 7.5g, calcium chloride 2.0g, magnesium sulfate 2.25g, potassium dihydrogen phosphate 750mg, inositol 750mg 125mg of ferrous sulfate, 100mg of manganese sulfate, 25mg of zinc sulfate, 25mg of boric acid, 2.5mg of potassium iodide, 1.0mg of sodium molybdate, 0.1mg of copper sulfate, fully stir to dissolve, mix well, and adjust the pH to 6.0. Take 5 more sterilized 2000mL beakers, and divide the above-mentioned solution equally among 5 beakers, each beaker is 1000mL.
[0033] 3. Inoculate 100mL of the seed solution of Bacillus subtilis, Pseudomonas fluorescens, Alcaligenes faecalis, Aspergillus niger and Saccharomyces cer...
Embodiment 2
[0036] 1. Select Bacillus sphaericus, Pseudomonas alcaligenes, Alcaligenes denitrificans, Aspergillus oryzae and Candida utilis, and carry out step-by-step seed expansion cultivation of the above-mentioned strains respectively.
[0037] 2. Add 2500mL of clean tap water to a sterilized container, add glucose 150g, potassium nitrate 6.25g, ammonium nitrate 5g, calcium chloride 1.25g, magnesium sulfate 1.25g, potassium dihydrogen phosphate 500mg, inositol 625mg , ferrous sulfate 87.5mg, manganese sulfate 62.5mg, zinc sulfate 25mg, boric acid 25mg, potassium iodide 2.5mg, sodium molybdate 0.75mg, copper sulfate 0.075mg, fully stir to dissolve, mix evenly, and adjust the pH to 8.0. Take 5 more sterilized 2000mL beakers, and divide the prepared solution into 5 beakers, each 500mL.
[0038] 3. Add 400 g of sterilized bran, 50 g of corn flour, and 50 g of bean cake powder to the beakers of the above 5 culture solutions, mix and stir evenly.
[0039] 4. Inoculate 50mL of the seed solu...
Embodiment 3
[0042] 1. Select Bacillus amyloliquefaciens and Clostridium saccharolyticus of the genus Bacillus, Pseudomonas aeruginosa and Pseudomonas denitrification of the genus Pseudomonas, Alcaligenes faecalis of the genus Alcaligenes and denitrification products For Alcalibacterium, Aspergillus niger and Aspergillus oryzae of the genus Aspergillus, and Saccharomyces cerevisiae and Candida utilis of the genus Saccharomyces, the above-mentioned bacterial species and each bacterial species were respectively subjected to step-by-step seed expansion cultivation.
[0043] 2. Add 2500mL of clean tap water to a container after disinfection, add glucose 125g, potassium nitrate 5g, ammonium nitrate 4.5g, calcium chloride 1.2g, magnesium sulfate 1.2g, potassium dihydrogen phosphate 450mg, inositol 500mg, 75mg of ferrous sulfate, 55mg of manganese sulfate, 20mg of zinc sulfate, 20mg of boric acid, 2mg of potassium iodide, 0.7mg of sodium molybdate, 0.07mg of copper sulfate, fully stir to dissolve,...
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