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Haynaldia villosa 1VS chromosome-specific molecular marker primer and application thereof

A technology of molecular markers and wheat tufts, applied in the field of molecular biology, can solve the problems of limiting wheat intraspecific quality and narrow genetic basis, and achieve the effect of simple detection method and accelerated breeding process

Inactive Publication Date: 2018-11-13
NORTHWEST A & F UNIV
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Problems solved by technology

Due to the narrow genetic basis of common wheat, there are not many types of high-quality protein genes found in common wheat, which limits the further improvement of wheat quality

Method used

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  • Haynaldia villosa 1VS chromosome-specific molecular marker primer and application thereof
  • Haynaldia villosa 1VS chromosome-specific molecular marker primer and application thereof

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Embodiment Construction

[0037] The terms used in the present invention, unless otherwise specified, are generally understood by those skilled in the art. The present invention will be described in further detail below in conjunction with specific embodiments. The examples are given only to illustrate the invention and not to limit the scope of application of the invention.

[0038] The specific molecular marker primers on the 1VS chromosome of the present invention are 1VS-F2 / 1VS-R3, 1VS-F8 / 1VS-R3, and the sequence of the molecular marker primers is: 1VS-F2: 5'-GTTTCCACCAAACAACCTTAT-3' (SEQ ID NO.1), 1VS-R3: 5'-TGCAGTGCTAAGTTCCTTATCTG-3' (SEQ ID NO.2); 1VS-F8: 5'-CTTCCTCATCTTTGTCCTCCTT-3' (SEQ ID NO.3).

[0039] Application of the 1VS chromosome-specific molecular marker primers 1VS-F2 / 1VS-R3 and 1VS-F8 / 1VS-R3 of the present invention in tracing the 1VS chromosome of T. villosa and cloning and isolating the gliadin gene sequence of the 1VS chromosome.

[0040] Application of the 1VS chromosome-specifi...

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Abstract

The invention discloses a haynaldia villosa 1VS chromosome-specific molecular marker primer and an application thereof. By the aid of the molecular marker primer, haynaldia villosa 1VS chromosomes ina wheat background can be accurately and rapidly identified, and haynaldia villosa prolamin genes can be separated. The technical scheme includes that the specific molecular marker primer comprises 1VS-F2 / 1VS-R3 and 1VS-F8 / 1VS-R3, the 1VS-F2 molecular marker primer has a nucleotide sequence as shown in a sequence table SEQ ID NO.1, the 1VS-R3 has a nucleotide sequence as shown in a sequence tableSEQ ID NO.2, and the 1VS-F8 has a nucleotide sequence as shown in a sequence table SEQ ID NO.3. The haynaldia villosa 1VS chromosome-specific molecular marker primers 1VS-F2 / 1VS-R3 and 1VS-F8 / 1VS-R3 are applied to haynaldia villosa 1VS chromosome detection and cloning and separation of the prolamin genes on the haynaldia villosa 1VS chromosomes.

Description

1. Technical field: [0001] The invention belongs to the field of molecular biology, and in particular relates to a molecular marker primer for identifying the 1V chromosome of P. villosa, a 1VS chromosome detection and a PCR method thereof. 2. Background technology: [0002] With the development of social economy and the improvement of living standards, people have higher and higher requirements for the quality of various noodles, so the quality improvement of wheat has also been paid more and more attention. Due to the narrow genetic basis of common wheat, there are not many genes encoding high-quality proteins in common wheat, which limits the further improvement of wheat quality. In order to find new high-quality gene resources, the excavation and utilization of high-quality protein genes of wheat relative species has become a hot spot in wheat quality improvement research (Yan et al., 2004; Li Qiaoyun et al., 2007; Yan et al., 2009;). [0003] Triticum genus Dasypyrum (...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/13C12Q2600/156C12Q2561/101
Inventor 李晓燕赵万春郭江岸高翔董剑杨璐霍丽花孟敏
Owner NORTHWEST A & F UNIV
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