12 results about "Protein network" patented technology

The embodiment of the invention provides a drug optimization method and system based on the overall effect of a protein network. The method comprises the following steps: constructing a protein interaction network related to a target disease, and dividing each protein target into a risk protein set and a protection protein set; respectively calculating first binding affinity data of the candidate drugs and each protein target in the risk protein set, and generating a first network comprehensive score based on the first binding affinity data; respectively calculating second binding affinity data of the candidate drugs and each protein target in the protection protein set, and generating a second network comprehensive score based on the second binding affinity data; calculating network confrontation scores of the candidate drugs according to the first network comprehensive score and the second network comprehensive score; and determining whether the candidate drug is a preferred drug based on the network adversarial score. The method can overcome the defect that a single-target drug is insufficient in curative effect due to a network compensation effect, so that safer and more effective candidate drugs are screened out.

The invention discloses an electrochemical sensor for glycoprotein detection and a glycoprotein detection method. The method comprises the following steps: anchoring an aptamer-protein conjugate on the surface of a gold electrode, and then assembling an anti-fouling peptide around the aptamer-protein conjugate to form an imprinted self-assembled monolayer film. And removing the combined protein through an acid solution to form a biocompatible imprinting cavity capable of being used for recombining a target object. The imprinted cavity can eliminate non-specific adsorption and enhance targeted binding efficiency. The sensor can be used for directly detecting carcino-embryonic antigens with the concentration as low as 0.1 ng / mL through an electrochemical impedance spectroscopy method. Besides, homodimer concanavalin A (ConA) is used as a recognition element and a cross-linking agent, homodimer glucose oxidase (GOx) is assembled on the surface of the electrode in situ, a protein network can be formed, and enzyme catalysis signal amplification detection is achieved. Through in-situ formation of a ConA-GOx assembly, the sensitivity is improved by 100 times.

The application relates to a low-temperature vacuum fresh noodle extruding mechanism and belongs to the technical field of food machines, which comprises a frame part serving as a support body and used for fixing and supporting various components of the whole fresh noodle extruding mechanism, a power part arranged at the rear end of the bottom end of the frame part and used for providing power for an extruding part so that the extruding part realizes the conveying and extruding of noodle flocculus, and the extruding part used for realizing the fresh noodle extruding process under low-temperature vacuum conditions, conveying the noodle flocculus through spiral extrusion, simultaneously extracting the air in the noodle flocculus through a vacuum pump, and controlling the temperature of the noodle flocculus through a cold water cavity. The fresh noodle produced by the method of controlling low temperature and extracting the air in the noodle flocculus in the spiral extrusion conveying process has more advantages, and the taste, cooking loss, elasticity, protein network structure and the like are greatly improved.

The invention belongs to the technical field of food processing, and relates to an application of a composite modified starch-based Pickering emulsion in 3D printing of surimi. Through a composite modification strategy of OSA modification and ball milling, the obtained composite modified starch is smaller in particle size and stronger in particle flexibility, and can be rapidly adsorbed and arranged on an oil-water interface to form a compact interface film, so that the Pickering emulsion has excellent stability; the composite modified starch-based Pickering emulsion is added into surimi to serve as printing ink, so that the rheological property and the extrudability of the surimi can be remarkably improved, and the printing shape fidelity is improved; starch particles in the emulsion absorb water to swell and fill protein network pores, so that the network structure of the minced fillet gel is enhanced, the water binding capacity is remarkably improved, the cooking loss is reduced, and the gel strength and microstructure uniformity are improved.

The invention relates to the technical field of gargle preparation, in particular to an oral gargle based on synthetic biological materials. Comprising the following steps: preparing a pre-crosslinked mussel protein adhesion network precursor solution, preparing a temperature-sensitive gel matrix, preparing a liquid main agent, preparing a nano-emulsion and preparing the oral gargle based on the synthetic biological material. Through sequential combination of tea polyphenol pretreatment and immobilized tyrosinase catalysis, the recombinant mussel byssus protein is subjected to directional chemical modification, in a weakly alkaline buffer solution, a catechol structure of tea polyphenol can be combined with a protein chain to play a role in pre-organization and stabilization, and the tea polyphenol plays a role in improving the stability of the mussel byssus protein while participating in network construction. The inherent antibacterial and antioxidant functions are integrated into a material matrix in situ, a mechanical stability foundation is created for the subsequent compounding of a protein network and temperature-sensitive hydrogel, the uniform coexistence of a protein phase and a hydrogel phase is ensured, and the wet adhesion strength of the protein material is essentially improved.

The present application relates to the technical field of bioinformatics, in particular to a biological information analysis system based on large model technology, which comprises a data analysis calibration module, a problem disintegration module, an analysis task arrangement module, a result mapping module and a feedback iteration module.In the present application, dynamic calibration of genome alignment and clinical phenotype timestamp eliminates time sequence misplacement deviation, base complementary pairing combined protein network abnormal screening enhances low-abundance collaborative variation capture, genotype-phenotype discrete distribution quantization unifies multi-modal data benchmark, solves multi-source heterogeneous standardization loss, classifies and integrates pathogenic gene semantic weight, balances statistical threshold and biological function, dynamically optimizes analysis sequence to synchronously cover key mutation area, improves non-coding region function annotation, integrates gene expression clustering and protein network topology in three-dimensional distribution, breaks through two-dimensional space limitation, and reduces genetic heterogeneity false positive rate through closed-loop feedback correction threshold iteration elimination rule.

The present invention relates to compositions and methods for subpopulation-specific modulation of cell function through spatiochemical and spatiotemporal control over intracellular regulatory protein networks at the level of expressed gene products of nucleic acid-binding protein (NABP) genes. Subpopulation-specific cytoactive agents of the invention are used in therapeutic compositions that up- or downregulate disease-associated intracellular regulatory protein genes at the RNA and protein level for prevention and treatment of plant, animal and human diseases. Applications in human clinical medicine include biopharmaceutical preparations and associated treatment methods for genetic, degenerative, neurological, cellular and immune disorders resulting from dysregulation of endogenous NABP regulatory networks. Therapeutic compositions designed or selected for use in plants and nonhuman animals are applied to the fields of agriculture and veterinary medicine. Also provided are research kits for subpopulation-specific modulation of protein activity and the corresponding potential to discover novel therapeutic compositions across fields. More specifically, the disclosed compositions and methods selectively up- or downregulate at least a first subpopulation of a cellular protein or therapeutic target without substantially altering the activity of at least a second subpopulation of the cellular protein or therapeutic target.

This invention discloses a Qingjin Huatan Decoction and its application in regulating the serum inflammatory factor protein network of patients with AECOPD and phlegm-heat accumulation in the lungs, belonging to the field of biomedical technology. The Qingjin Huatan Decoction of this invention is composed of the following raw materials in parts by weight: Scutellaria baicalensis 10-20 parts, Gardenia jasminoides 6-12 parts, Morus alba root bark 6-12 parts, Trichosanthes kirilowii 10-14 parts, Fritillaria thunbergii 6-12 parts, Ophiopogon japonicus 6-12 parts, Platycodon grandiflorus 10-20 parts, Anemarrhena asphodeloides 6-12 parts, Citrus reticulata peel 10-20 parts, Poria cocos 10-20 parts, and Glycyrrhiza uralensis (processed) 4-8 parts. This formula is consistent with the core pathogenesis of AECOPD and phlegm-heat accumulation in the lungs, with appropriate warming and cooling properties, and a combination of tonifying and purging effects. It can inhibit the proliferation of pathogenic bacteria in the intestines, correct intestinal flora imbalance, achieve intestinal-lung harmony through the intestinal-lung axis, comprehensively improve patients' clinical symptoms, and enhance clinical suitability.

The present invention relates to methods and compositions for the high throughput screening of protein-protein interactions in yeast liquid culture. Protein fusions non-native to yeast may be expressed to replace endogenous sexual agglutination proteins and mediate library-by-library interrogation of protein interactions. The methods and compositions of the invention can be utilized for the characterization of protein interaction networks in high throughput for both binding affinity and specificity, which is crucial for understanding cellular functions, screening therapeutic candidates, and evaluating engineered protein networks.

The invention discloses a biomarker of amyotrophic lateral sclerosis. On the first aspect, the invention provides application of a substance for detecting a biomarker in preparation of a diagnostic product for amyotrophic lateral sclerosis, and the biomarker comprises at least one of Lyz2, Mup20, Lgals3, Sfn, Apod, Apoe, C1QA, C1QB, SPA3N, CD44 and MT2. According to the scheme, proteomics detection is carried out on medulla oblongata and spinal cord areas of ALS mice and WT mice, a spinal cord and medulla oblongata differential protein network of an ALS pathological model is established, potential biomarkers are screened out, and ALS diagnosis is facilitated through research on the biomarkers.