Method for preparing chitosan soluble gauze rich in growth factors

Abstract

The invention discloses a method for preparing chitosan soluble gauze rich in growth factors. The method comprises the steps of S1, weighing auxiliary materials according to a weight ratio to prepare blood platelet pretreatment liquid; S2, preparing blood platelet freeze-dried buffer solution; S3, preparing pretreated blood platelet rich plasma; S4, preparing carboxymethyl chitosan solution; and S5, preparing the chitosan soluble gauze rich in growth factors. After chitosan is gelated, the blood platelet rich plasma is added to obtain the chitosan soluble gauze rich in growth factors, pore diameter is 130-160 [mu]m, a large number of growth factors and abundant fibrous protein networks are contained and can be absorbed by the human body easily, no toxic and side effects can be caused, growth of various bacteria and fungi can be restrained, more structure space is provided for cell growth, in-vivo and in-vitro release of various growth factors can be achieved, tissue repair and vascular remodeling can be achieved easily, antibacterial performance is excellent, and the chitosan soluble gauze is a novel medical and biologically hemostatic material with high cost performance.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a preparation method of chitosan soluble gauze rich in growth factors. Background technique [0002] Hemostasis is an important step in emergency medical treatment. Patients need rapid hemostasis when there is sudden trauma during surgical treatment and daily life. Wound hemostasis during surgery and dressing change for postoperative wounds are very important links. Traditional dressings such as gauze and wound towels have poor adsorption, antibacterial effect, compatibility with the body, air permeability, and moisture permeability. There are defects. The wound granulation tissue is easy to grow into the mesh of the dressing. When the dressing is changed, it is easy to adhere to the wound of the wound, damaging the new granulation tissue and epithelial tissue, causing re-injury and bleeding, increasing the chance of bacterial infection, and not only reducing it The speed of wou...

AI Technical Summary

Problems solved by technology

Chitosan is also made into a gel, but the pore size is about 50 μm, which cannot provide space for cell growth and nutrient blood vessels, and affects the infiltration of cell secretion matrix and nutrients, so it is only suitable for hemostasis on the skin surface, and cannot meet the requirements for hemostasis in the body. Require

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