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Method for preparing corn peptide by using corn gluten water

A corn gluten and corn polypeptide technology, which is applied to the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of low nutritional value, low water solubility, poor processability, etc., and achieve low production costs. , Technical scheme design scientific, good quality effect

Inactive Publication Date: 2014-04-09
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The protein in corn gluten water is mainly alcohol-soluble protein, but because it lacks essential amino acids such as tryptophan and lysine, and contains a high proportion of non-polar amino acid residues, it determines that its nutritional value is not high, and its water solubility is not high. Strong, poor processability, less demand in the human edible protein market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Use sodium sulfite to adjust the pH value of the corn gluten water to 5.5, heat to 90°C, and centrifuge for 3 hours with an ultrasonic power of 1000W. Collect the liquid, adjust the pH value of the centrifuge solution to 11 with sodium hydroxide, add 0.4% of the centrifuge solution weight Alkaline protease, enzymatically hydrolyze at 60℃ for 4 hours. After the enzymolysis is completed, the enzymatic hydrolysate is passed through a 0.2 micron microfiltration membrane to remove impurities. The working conditions of the microfiltration membrane for impurity removal are: pressure 0.3MPa and temperature 50℃; the filtrate of the microfiltration membrane has a molecular weight cut-off of 800Dal. Nanofiltration membrane concentration, the working conditions of nanofiltration membrane concentration are: pressure 1.5MPa, temperature 50℃; the concentrated solution of nanofiltration membrane is dried to obtain corn polypeptide. At this time, the yield of corn peptides was 87%, the p...

Embodiment 2

[0046] Use sodium sulfite to adjust the pH value of the corn gluten water to 8.0, heat up to 70°C, and centrifuge at 500W ultrasonic power for 2 hours. Collect the liquid, adjust the pH value of the centrifuge solution to 8 with sodium hydroxide, add 0.1% of the centrifuge solution weight Alkaline protease, enzymatically hydrolyze at 60℃ for 2 hours. After the enzymolysis is completed, the enzymatic hydrolysate is passed through a 0.4 micron microfiltration membrane to remove impurities. The working conditions of the microfiltration membrane for impurity removal are: pressure 0.1MPa and temperature 40°C; the filtrate of the microfiltration membrane passes through a molecular weight cut-off of 1000Dal Nanofiltration membrane concentration, the working conditions of nanofiltration membrane concentration are: pressure 1.0MPa, temperature 40℃; the concentrated solution of nanofiltration membrane is dried to obtain corn polypeptide. At this time, the yield of corn peptide was 85%, t...

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Abstract

The invention discloses a method for preparing a corn peptide by using corn gluten water. The method comprises the steps: regulating the pH value of the corn gluten water to 5.5-8.0 by using sodium sulfite, then, carrying out ultrasonic treatment, and centrifugally collecting a liquid; regulating the pH value of the liquid to 8-11 by using sodium hydroxide, and adding alkaline protease to carry out enzymolysis; and removing impurities in an enzymatic hydrolysate through a microfiltration membrane, then, concentrating the microfiltration filtrate through a nanofiltration membrane, and carrying out drying treatment on a concentrate to obtain the corn peptide. The method is used for producing the corn peptide through extracting by taking the corn gluten water as the raw material, so that the production cost is relatively low; through pH regulation and ultrasonic treatment, prolamin is modified before enzymolysis is finished, so that the purity of a protein and the hydrolysis effect are improved; through two-stage membrane separation treatment, a corn peptide product containing 90% of proteins is obtained, and the process yield is about 85%; extraction is realized without using an organic solvent, and the corn peptide product is free of solvent residues and high in safety; the COD (Chemical Oxygen Demand) of a small deal of residual wastewater is less than or equal to 800mg / L, and the wastewater can be discharged up to the standard after being treated a little.

Description

[0001] technical field [0002] The invention relates to the preparation of corn polypeptide, in particular to a method for preparing corn polypeptide from corn gluten water. Background technique [0003] Corn (scientific name: Zea mays), also known as maize, corn, corn, and cob, is an annual herbaceous plant that harvests mature fruits in summer and autumn. Corn is one of the three major food crops in the world, and it is also the food crop with the highest total output in the world. It is cultivated in all parts of China and is a traditional crop in my country. Maize is a staple food in many regions, and it is also used as feed and as a major raw material for the production of starch and ethanol. [0004] During the production of corn into starch, a by-product called corn gluten water is separated by the starch separator. In the corn starch processing industry, for every 10 tons of corn processed, 7-8 tons of corn gluten water will be obtained. The water content of corn...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 万端极李冬生吴正奇徐国念李祝黄磊李猷
Owner HUBEI UNIV OF TECH
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