Fast appraisal method for purity of Brassica napus L. hybrid seed

Abstract

The invention discloses a method for approving the purity of cabbage type rape hybrid seed. The method comprises the steps as follows: the seed is squeezed and is soaked in 2-chlorohydrin solution for 2 to 3 hours for picking up prolamin; then electrophoretic separation is carried out by separation gel and stacking gel; the processes of fixing, dying, decoloring and banding are carried out after electrophoresis; and finally, the purity of the rape hybrid seed is calculated by tape reading. The approving method has the advantages of simple operation, low cost, high efficiency, high accuracy, etc.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding and application, and in particular relates to a method for identifying the purity of rapeseed hybrid seeds. Background technique [0002] Hybrid rapeseed, as the main breeding technology to promote the development of rapeseed production in my country, has achieved rapid development in recent years. With the increasing popularity of hybrid rapeseed, the quality control of seed purity is becoming more and more important, especially for the hybrid type mainly composed of cytoplasmic male sterility. The female parent of this sterile type is sensitive to temperature. It is easy to produce a small amount of pollen, and produce sterile female-type pseudo-hybrids by self-crossing, which can cause a large reduction in hybrid rapeseed production in severe cases. Therefore, strict purity identification must be carried out before rapeseed hybrids are used in production. [0003] Seed purity identi...

AI Technical Summary

Problems solved by technology

However, the isoenzyme identification method requires rapeseed seedling cultivation, and it takes several days from germination to emergence, and the isoenzyme electrophoresis bands are blurred, which is easy to misread and cause deviations in the identification results, which brings a lot of inconvenience to the purity identification

The prolamin electrophoresis technique uses seeds directly as materials for analysis without the need for seedling cultivation. As a stored protein electrophoresis technique, it has been applied in the identification of seed purity of gramineous crops such as wheat, corn, and rice. The protein content is very small, and it is difficult to detect by electrophoresis and staining methods widely used in grass crops, while other biochemical identification and molecular marker methods have complicated operation steps, high cost, and long detection cycle

So far, there has been no report on the identification of the purity of hybrid rapeseed seeds using gliadin electrophoresis

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