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Optimal enzyme preparation application system (OEATM)

A technology of enzyme preparation and system, which is applied in the field of quantitative and individualized application of compound enzyme preparation for feed, can solve the problems of ineffective addition of enzyme preparation, high addition cost, and not completely suitable for diet formula design

Active Publication Date: 2011-11-16
北京华美源生物科技有限公司
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Problems solved by technology

The main reason may be that after the addition of enzyme preparations, the original feed database and animal nutrition requirements parameters are not suitable for the actual situation (Feng Dingyuan and Shen Shuibao, 2005), resulting in the ineffective effect of adding enzyme preparations or the high cost of adding enzyme preparations
They believe that after adding enzyme preparations to feed, the existing feed raw material database and even feeding standards may not be completely suitable for the diet formula design using enzyme preparations

Method used

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  • Optimal enzyme preparation application system (OEATM)
  • Optimal enzyme preparation application system (OEATM)
  • Optimal enzyme preparation application system (OEATM)

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Embodiment Construction

[0029] The present invention mainly has two contents: evaluation of effective enzyme activity and evaluation of effective nutritional value.

[0030] 1. Evaluation of effective enzyme activity

[0031] (1) Determination of labeled enzyme activity of enzyme preparations

[0032] Take a certain amount of enzyme sample solution, dissolve it with 0.1mol / L NaAc-HAc buffer solution of pH 5.5, and then dilute the sample solution to an appropriate multiple according to the enzyme activity (it is best to make the diluted enzyme solution to be tested Enzyme activity can be controlled between 0.04-0.08U / mL). Then put the diluted enzyme solution and the prepared substrate solution in a water bath at 37°C for 30 minutes, then add DNS reagent to terminate the enzyme reaction, take a boiling water bath for 5 minutes, and cool to a constant volume. Set the standard blank to zero, measure the absorbance value at 540nm with a spectrophotometer, and finally calculate the enzyme activity accord...

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Abstract

The invention relates to an optimal enzyme preparation application system (OEATM). The system comprises effective enzyme activity evaluation and effective nutrient value evaluation, wherein the effective enzyme activity evaluation comprises six indexes, namely labeled enzyme activity, a pH threshold value, excess gastric acid measurement, excess protease measurement, high-temperature resistance measurement and a three-month enzyme activity retention rate; and the effective nutrient value evaluation comprises four indexes, namely feed viscosity measurement, feed energy value measurement, feed protein digestibility measurement and animal growth performance. By a new idea and a new method, the problems in the application process of a feed enzyme preparation are solved; and experiments prove that the OEATM has higher pertinence to a complex enzyme and lower cost.

Description

field of invention [0001] The invention belongs to the field of feed additives. It particularly relates to the quantitative and individualized application of compound enzyme preparations for feed. Background technique [0002] The addition and application of enzyme preparations in feed is the most concerned field of application of biotechnology in the feed industry. From the addition of enzyme preparations to feed in the 1950s, to the widespread application of feed enzyme preparations in the 1990s, the role of enzyme preparations in livestock and poultry farming has become increasingly prominent. At the same time, surveys show that only 10% of all monogastric animal feeds in the world add enzymes, and the amount of enzymes used in pig feeds is much lower than that in poultry feeds. According to the existing research data, there are two main reasons affecting the rapid development of feed enzymes: one is the standardization of the single enzyme preparation evaluation system...

Claims

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Application Information

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IPC IPC(8): C12Q1/00C12Q1/37C12Q1/34A23K1/165A23K20/189
Inventor 王海彦唐茂妍孙鸣陈旭东徐玲王纪亭
Owner 北京华美源生物科技有限公司
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