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In vitro method for thoroughly evaluating protein biomass amount in babies' formula milk powder

An infant formula and biological quality technology, applied in the field of protein biological quality evaluation, can solve the problems such as the inability to calculate the digestion of each amino acid, the lack of comparison of the recommended amounts, the failure to reflect the changes in gastric acidity and the speed of gastrointestinal peristalsis.

Inactive Publication Date: 2009-04-22
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also a small number of people in China who have carried out some research on the in vitro digestion of milk protein. For example, Chen Jing used an enzyme-membrane reactor to hydrolyze the protein in milk in vitro, and P W Jones in the United States conducted a research on the quality of milk protein in baby food. The evaluation of in vitro simulation is only done by adding different animal proteases and plant proteases, which does not reflect the change of acidity in the stomach and the speed of gastrointestinal peristalsis, and only calculates the digestibility of total amino acids, not the digestion of each amino acid. , there is no comparison for recommended amounts

Method used

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  • In vitro method for thoroughly evaluating protein biomass amount in babies' formula milk powder
  • In vitro method for thoroughly evaluating protein biomass amount in babies' formula milk powder
  • In vitro method for thoroughly evaluating protein biomass amount in babies' formula milk powder

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Experimental program
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Embodiment Construction

[0027] The following examples describe the present invention in more detail:

[0028] 1. Evaluation of Sample Processing

[0029] Taking commercially available infant formula milk powder as the processing object, the protein content in the milk powder was measured and prepared into a 12% liquid sample; 100 ml of the sample was taken and preheated and kept at 37°C for use.

[0030] 2. Preparation and use of gastric juice

[0031] The gastric juice is composed of 0.8mg / ml pepsin, 1.2mg / ml rennet, and 0.088mol / LH + composition. Use the method described in Table 3

[0032] table 3

[0033]

[0034] After the gastric juice was treated for 1 hour, the pH was adjusted to neutral with 1M sodium hydroxide solution, and the above process was completed by peristaltic stirring with a peristaltic pump.

[0035] 3. Preparation and use of intestinal juice enzymes

[0036] 0.8g trypsin, 1.2g rennet and then add 5g bile salt. Intestinal juice enzymes were added to the gastric juice-t...

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Abstract

The invention provides an in-vitro method for comprehensive assessment of protein biological quality in infant formula milk powder. The infant milk powder is firstly re-dissolved to detect total proteins and total acid hydrolyzed amino acids, then the infant milk powder is digested by artificial simulated gastric juice and intestinal juice and precipitated by trichloroacetic acid with the concentration of 10 percent, and soluble nitrogen and amino acids in supernatant liquor are detected. The protein digestibility and the essential amino acid digestibility are calculated through the detection results, the modified amino acid score is obtained by multiplying the total amount of essential amino acids in the supernatant liquor and a model contrast value which is recommended by WHO by the essential amino acid digestibility, and the protein biological quality is determined by the value which is obtained by multiplying the protein digestibility by the modified amino acid score.

Description

(1) Technical field [0001] The invention relates to a method for evaluating protein biomass, in particular to an in vitro method capable of comprehensively evaluating protein biomass in infant formula milk powder. (2) Background technology [0002] Infant formula generally contains casein and whey proteins to ensure that infants fed the formula absorb adequate amounts of amino acids, especially essential amino acids. Existing baby milk powder can achieve the same level as breast milk in terms of nutritional components and trace components in terms of quantity, or even exceed breast milk, but there is a big difference in quality, mainly because the protein is not easy to digest, and the potency is low. Easy to cause baby allergies such as diarrhea, vomiting and so on. In order to pursue profits, some unscrupulous manufacturers add some non-dairy proteins and even starches to infant formula. These proteins are not easily digested and absorbed by infants, which seriously reduc...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 霍贵成郭鸰任大喜
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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