Determination method of four common unsaturated fatty acids in infant milk powder

A technology of unsaturated fatty acids and infant milk powder, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as complexity, affecting work efficiency, and high reagent consumption, achieving good separation, good linearity, repeatability and recovery rate high effect

Inactive Publication Date: 2011-12-21
WISSUN INT NUTRITION GRP
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The existing standard methods for the determination of unsaturated fatty acids in milk powder have different requirements for sample pretreatment and detection methods. work efficiency

Method used

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  • Determination method of four common unsaturated fatty acids in infant milk powder
  • Determination method of four common unsaturated fatty acids in infant milk powder
  • Determination method of four common unsaturated fatty acids in infant milk powder

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Embodiment Construction

[0013] The present invention is described in detail below in conjunction with embodiment

[0014] 1. Materials and methods

[0015] 1.1. Materials and Instruments

[0016] 1.1.1. Raw materials and reagents

[0017] Infant milk powder, Mingyi (Fujian) Infant Nutrition Co., Ltd.;

[0018] Various fatty acid standard products were purchased from Sigma Company;

[0019] Potassium hydroxide, sodium chloride, anhydrous sodium sulfate, absolute ethanol, anhydrous ether, and petroleum ether are all analytically pure (Tianjin Yongda Chemical Reagent Co., Ltd.), and the volume fraction is 25% ammonia water (excellent grade, Tianjin Kemiou Chemical Reagent Co., Ltd.), boron trifluoride methanol solution (mass fraction is 14%, CNW), heptane (chromatographically pure, CNW), n-hexane (chromatographically pure, TEDIA), and experimental water is ultrapure water .

[0020] 1.1.2. Experimental Instruments

[0021] Gas chromatograph (Focus, Thermo Fisher Scientific Corporation), with FID d...

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Abstract

The invention discloses a method for detecting four common unsaturated fatty acids in infant milk powder, which comprises: weighing infant milk powder samples, dissolving in hot water and shaking; adding ammonia water after the water is cooled to room temperature; Shake, after cooling to room temperature, add absolute ethanol and absolute ether to separate the ether layer from the water layer; extract the ether layer, add absolute ethanol, absolute ether and petroleum ether to separate the ether layer from the water layer; Extract the ether layer, add anhydrous diethyl ether and petroleum ether to separate the ether layer and the water layer; combine the ether layer liquids extracted three times above, concentrate, dissolve the residue with heptane, add potassium hydroxide methanol solution to reflux, add Boron trifluoride methanol solution continues to heat and reflux; stop heating and cool to room temperature, adjust pH<1.5, add saturated NaCl solution to separate layers; pipette the upper organic phase, dehydrate with anhydrous sodium sulfate, and use a 0.45 μm filter membrane for the supernatant Filtration, gas chromatography determination, low detection limit, good linearity.

Description

technical field [0001] The invention relates to a detection method for four common unsaturated fatty acids in infant milk powder, in particular to a detection method for linolenic acid, linoleic acid, arachidonic acid and docosahexaenoic acid in infant milk powder. Background technique [0002] Unsaturated fatty acids are important essential fatty acids for the human body. Linolenic acid (LA), linoleic acid (LNA), arachidonic acid (AA) and docosahexaenoic acid (DHA) are common unsaturated fatty acids. Scientific research in recent years has shown that unsaturated fatty acids are important nutrients for the brain and cranial nerves, and play a vital role in the development of infants and young children. They can improve infants' intellectual development index and visual acuity. memory and thinking. Some unsaturated fatty acids cannot be synthesized by the human body and need to be ingested through food. Therefore, the determination of unsaturated fatty acid content in infan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 陈光耀
Owner WISSUN INT NUTRITION GRP
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