Alpha-1, 2-fucosyltransferase and application thereof in milk powder

A technology of glycosyltransferase and fucosyl, applied in the field of bioengineering, can solve the problems of cumbersome operation, difficult separation and purification, and low yield of chemical synthesis methods, and achieve enhanced immunity, great commercial value, and high enzyme activity Effect
CN109337883AInactive Publication Date: 2019-02-15FUJIAN AGRI & FORESTRY UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUJIAN AGRI & FORESTRY UNIV
Publication Date
2019-02-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides an alpha-1, 2-fucosyltransferase and an application thereof in a milk powder and belongs to the technical field of gene engineering. The invention relates to a nucleic acid andan amino acid sequence from thermophilic synechococcus (Thermosynechococcus sp. Nk 55a); the alpha-1, 2-fucosyltransferase is encoded; and obtained groups are separated to construct an expression vector pET15b-Tnfuct and converted into Escherichia coli strain BL21(DE3) to realize efficient expression. A method for generating fucosylated oligosaccharide, glycopeptide and glycoprotein by using the alpha-1, 2-fucosyltransferase has an application prospect in the industrial fields of infant milk powder, foods and medicines.
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Description

technical field

[0001] The invention relates to an alpha-1,2-fucosyltransferase and its application in milk powder, belonging to the technical field of bioengineering. Background technique

[0002] Breast milk is the best food for newborns. It can not only provide the best nutrition for newborns, but also provide them with biologically active substances needed for growth and development. These natural active substances are beneficial for the colonization of the intestinal microbiota of newborns, the maturation of the immune system and systems, and the development of cognitive abilities. A large number of studies have found that human milk oligosaccharides (Human milkoligosaccharides, HMOS) is an important active factor in breast milk, which plays an extremely important role in the healthy growth of infants, can promote the proliferation of intestinal probiotics in infants, and resist pathogenic bacteria and virus invasion, as well as regulating the immune system. Neutral o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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