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34results about How to "Colonization fast" patented technology

Preparation method of liquid fertilizer for preventing and treating banana wilt

The invention discloses a preparation method of a liquid fertilizer for preventing and treating banana wilt.The preparation method comprises the following steps that 1, lactic acid bacteria accounting for 0.1%-0.5% of the weight of molasses-alcohol concentrated waste liquid and saccharomycetes accounting for 0.01%-0.05% of the weight of the molasses-alcohol concentrated waste liquid are added into the molasses-alcohol concentrated waste liquid, the materials are mixed to be uniform, and after natural fermentation is performed for 3 months, molasses-alcohol fermentation liquid is obtained; 2, 5-30 parts by weight of cassava extracting liquid, 2-20 parts by weight of nitrogen-phosphorus-potassium fertilizer, 0.1-5 parts by weight of medium trace elements and 5-40 parts by weight of the molasses-alcohol fermentation liquid prepared in the step 1 are put into a reactor, water is added to supplement the total parts by weight to 100, then beneficial bacteria are added, the materials are stirred to be uniform to enable the density of the beneficial bacteria to be 0.5-2 hundred million per milliliter, and the liquid fertilizer is obtained.The liquid fertilizer prepared through the method contains allelopathy inhibiting substances of fusarium oxysporum and has the positive effect on prevention and treatment of the banana wilt.
Owner:南宁汉和生物科技股份有限公司

Method for cultivating edible fungi by means of wood blocks

The invention discloses a method for cultivating edible fungi by means of wood blocks. A matrix of a traditional strain culture medium is changed, the matrix of strains and the matrix of the wood blocks are the same and can be fused into a whole, and harmonious coexistence can be achieved; the strains and a strain cover are combined into a whole and all communicated with xylon of the wood blocks, and the disengagement problem of a traditional strain cover is solved thoroughly. According to the technical scheme, the method for cultivating the edible fungi by means of the wood blocks comprises the following steps of wood block preparation, wherein timber is cut into long wood blocks for use, according to differences of cultivated varieties, the tree varieties of pine and Chinese fir are selected for ganoderma tsugae, and broadleaf wood is selected for other varieties; wood block strain making; wood block punching, wherein punching is conducted through a mechanical punching machine or an electric drill or a handmade hammer, and the hole depth, hole diameter, line spacing and spacing are determined according to the cultivated varieties and tree varieties; strain inoculation, wherein wood block strains are embedded in wood block holes, and tamping is conducted with a hammer till no gap exists between the hole walls and the strain blocks; spawn running, wherein a spawn running way is determined according to the technological requirements of different varieties of cultivated edible and medical fungi, differences of the tree species and the specific circumstances of sites; fruiting management.
Owner:宫晶晶

Method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus

The invention discloses a method for preparing cultivars of artificially-domesticated red-soil termitomyces albuminosus, which is the practical application of an artificial domestication biotechnology, and solves the technical problem of artificial cultivation of wild fungi. The technical method comprises the following steps: (1) preparing a raw material of a cultivar culture-medium, and adding water into the raw material, so that the water content of the obtained product is 40-60%, wherein the raw material comprises the following components in part by weight: 50-90 parts of food grains, 8-48 parts of sawdust, one part of monopotassium phosphate, and one part of sugar; (2) preparing the raw material of the cultivar culture-medium; (3) preparing a feed of the cultivar culture medium; and (4) inoculating the feed to artificially-domesticated original strains of red-soil termitomyces albuminosus through sterile operation to carry out light-tight cultivation, and after 25-65 days, mycelium are overgrown in a culture flask and become mature stock seeds, carrying out propagation on the mature stock seeds so as to obtain the cultivars of red-soil termitomyces albuminosus. The cultivars of red-soil termitomyces albuminosus disclosed by the invention can be used for artificially planting and producing the red-soil termitomyces albuminosus.
Owner:云南福保农业科技开发有限公司

Grifola frondosa three-stage culture production method

The invention provides a grifola frondosa three-stage culture production method. The grifola frondosa three-stage culture production method comprises the steps of first-stage culture production, second-stage culture production, third-stage culture production, culture package production, inoculation and cultivation, bud forcing and mushroom cultivation. The third-stage culture production comprises the steps of obtaining of culture medium recipes, production of the culture media, bagging, sterilization, inoculation, cultivation of the culture and raising of the culture. The grifola frondosa three-stage culture production method is characterized in that the culture medium recipes include the wood strip culture medium recipe and the wheat grain culture medium recipe, and the production of the culture media comprises production of a wood strip culture medium and production of a wheat grain culture medium. Compared with a wood chip culture, the culture obtained through the grifola frondosa three-stage culture production method has the advantages that inoculation efficiency is 5-8 percent higher, the maximum culture growth distance is 50-100 percent shorter, the cultivation efficiency is 20-60 percent higher, each culture bag is consistent in mycelial age vertically, so that nutrients are released in a centralized mode, and the first-batch mushroom yield can be increased by 15-30 percent. Compared with liquid culture, the culture obtained through the grifola frondosa three-stage culture production method is applicable to existing culture production and detection, inoculation equipment and technicians in China, and can reduce the cultivation infection rate by 2-5 percent.
Owner:HUNAN RES INST OF EDIBLE FUNGI +1

Formula for cultivating fungi

The invention discloses a formula for cultivating fungi. The formula comprises, by weight, 55 g of rice bran, 55 g of wheat bran, 305.1 g of saw dust, 2.5 g of shell ash and 104.6 g of water, whereinthe sum of the content of the rice bran, wheat bran and saw dust is 229.6 g; a method for processing the formula comprises the steps of stirring, adding water, sterilizing and cooling, wherein the method comprises the specific steps of: stirring, adding the rice bran, wheat bran, saw dust and shell ash into a vessel sequentially, and mixing the powder; adding the water after the powder is mixed evenly, and mixing the obtained mixture; sterilizing, adding the obtained mixture into a culture vessel, heating the culture vessel to 85 DEG C for sterilization, heating the culture vessel to 100 DEG Cfor second sterilization, and finally heating the culture vessel to 110 DEG C for sterilization, and finally keeping the temperature for 80 minutes; after the sterilization, cooling the culture vessel to 20 DEG C for cultivation for 18 days. Compared with the prior art, the formula has the advantages that the raw material of the culture medium are cheap, easy to buy and convenient to store, the method for preparing the culture medium is simple, and hypha grows vigorously and has high activity; after the inoculation of the hypha onto a culture material, planting is quick, and the rate of pollution is low.
Owner:福建万辰生物科技股份有限公司

High-pressure vapour sterilization method of mushroom stick

The invention discloses a high-pressure vapour sterilization method of a mushroom stick. The method comprises the steps that (1),the mushroom stick is punctured, a breathable film is bonded to the mushroom stick, and the breathable film is sealed after the mushroom stick is placed in a high-temperature sterilization device; (2), vapour is added, an air inlet valve is adjusted to a lower magnitudewhen the pressure is increased to 0.9 kg/ cm <2>, an air discharging valve is switched on gradually, the air inlet valve is completely switched on when the pressure drops to zero, the state of the airinlet valve is maintained for 3-5 min until dense vapour is discharged out from an air discharging port, the air discharging valve is switched off, and the air discharging operation is repeated; (3),when the vapour pressure is increased to 0.8 kg/ cm <2> and the temperature is increased to 108 DEG C +/- 1 DEG C, the air inlet valve is adjusted to stabilize the pressure and the temperature, and the pressure and the temperature are maintained for 4 hours; (4), the vapour pressure is adjusted from 0.8 kg/ cm <2> to 0.4 kg/ cm <2> within 0.8-1 hour, the temperature is 105 DEG C +/- 1 DEG C, andthe pressure and the temperature are stabilized for 5 hours; (5), the air inlet valve is switched off, the air discharging valve is switched on when the vapour pressure is naturally reduced to 0.1 kg/cm <2>, the air discharging valve is switched off when no vapour is discharged from the air discharged valve, and the sterilization is completed.
Owner:SHANXI AGRI UNIV

Preparation method and application of probiotic spore Capsid Nano-materials and probiotic composition

The invention relates to a preparation method and application of a probiotic spore Capsid Nano-materials and probiotic composition. The problem that various gastric acid-intolerant probiotics are inactivated or reduced in activity after being orally taken through the stomach, and then the intestinal colonization efficiency is low can be effectively solved, growth and proliferation of harmful bacteria are inhibited, the intestinal barrier is recovered and rebuilt, the intestinal flora is adjusted, and then the colon cancer related to colitis and colitis is effectively prevented and treated. Thepreparation method comprises the steps that firstly, probiotics are cultured to generate spores, then spore Capsid is extracted through an extruder and prepared into the Capsid Nano-materials (CNMs)uniform in particle size and stable in property, then the Capsid Nano-materials and the probiotics are co-incubated in a growth culture medium, and the spore Capsid Nano-materials and probiotic composition is obtained. The preparation method is simple, low in production cost and stable in gastric acid environment, the intestinal flora is adjusted, the intestinal barrier is enhanced, and a new technical support is provided for treatment of inflammatory bowel diseases and inflammation induced cancers.
Owner:ZHENGZHOU UNIV

A kind of high pressure steam sterilization method of mushroom stick

The invention discloses a high-pressure vapour sterilization method of a mushroom stick. The method comprises the steps that (1),the mushroom stick is punctured, a breathable film is bonded to the mushroom stick, and the breathable film is sealed after the mushroom stick is placed in a high-temperature sterilization device; (2), vapour is added, an air inlet valve is adjusted to a lower magnitudewhen the pressure is increased to 0.9 kg / cm <2>, an air discharging valve is switched on gradually, the air inlet valve is completely switched on when the pressure drops to zero, the state of the airinlet valve is maintained for 3-5 min until dense vapour is discharged out from an air discharging port, the air discharging valve is switched off, and the air discharging operation is repeated; (3),when the vapour pressure is increased to 0.8 kg / cm <2> and the temperature is increased to 108 DEG C + / - 1 DEG C, the air inlet valve is adjusted to stabilize the pressure and the temperature, and the pressure and the temperature are maintained for 4 hours; (4), the vapour pressure is adjusted from 0.8 kg / cm <2> to 0.4 kg / cm <2> within 0.8-1 hour, the temperature is 105 DEG C + / - 1 DEG C, andthe pressure and the temperature are stabilized for 5 hours; (5), the air inlet valve is switched off, the air discharging valve is switched on when the vapour pressure is naturally reduced to 0.1 kg / cm <2>, the air discharging valve is switched off when no vapour is discharged from the air discharged valve, and the sterilization is completed.
Owner:SHANXI AGRI UNIV

Tobacco bacterial wilt prevention and growth promotion compound microorganism effervescent tablet as well as preparation method and application thereof

The invention discloses a tobacco bacterial wilt prevention and growth promotion compound microorganism effervescent tablet as well as a preparation method and application thereof. The compound microorganism effervescent tablet is prepared from the following raw materials in parts by weight: 5-20 parts of bacterial powder, 5-20 parts of tartaric acid, 10-50 parts of sodium bicarbonate, 5-30 partsof polyethylene glycol, 2-20 parts of lactose, 10-40 parts of microcrystalline cellulose, 1-10 parts of magnesium stearate, 1-10 parts of bletilla striata extract and 3-8 parts of trehalose. Accordingto the invention, the compound microbial effervescent tablet prepared from the tobacco growth-promoting strain bacillus velezensis and the tobacco bacterial wilt antagonistic strain bacillus subtiliscan effectively inhibit tobacco bacterial wilt caused by ralstonia solanacearum. The bletilla striata extract is added, so that rapid forming of the effervescent tablet can be effectively promoted, the activity of the strains is kept, and the validity period of the effervescent tablet is prolonged. By adjusting the composition and structure of soil and rhizosphere microorganisms, the content of beneficial microorganisms is increased, pathogenic bacteria are effectively inhibited, and the purposes of promoting tobacco growth and effectively resisting bacterial wilt are achieved.
Owner:ZUNYI MEDICAL UNIVERSITY +1

Hydroponic device

PendingCN112005874AAvoid root entanglementFree combination control planting densityAgriculture gas emission reductionCultivating equipmentsPlant stalkPlant stem
The invention discloses a hydroponic device. The hydroponic device is formed by combining a barrel body on the lower portion and a contraction opening in the upper portion, an arc-shaped structure isarranged at the upper portion of the barrel body, an adjusting structure is arranged at the lower portion of the contraction opening; during use, the adjusting structure is clamped with the arc-shapedstructure, hard connecting rods are evenly arranged on the upper edge of the arc-shaped structure along the barrel body, elastic grooves are formed in the hard connecting rods, holes matched with theelastic grooves are formed in the barrel wall of the barrel body, the elastic grooves can block the holes, hollow ox horn structures are arranged at the tail ends of the hard connecting rods, and thehollow ox horn structures are connected end to end and can be fixed into a ring; and the contraction opening is cut off downwards from the upper end, and sponge is arranged in the contraction openingand used for fixing plants. The hydroponic device solves the problem that a hydroponic device set cannot be disassembled to adapt to various experimental conditions, solves the problem of root winding after hydroponic plants are dense, solves the lodging phenomenon after plant stalks extend, can control the depth of the plants immersed in water, prevents root rotting, is convenient and elastic toopen a contraction opening, and can be used for quickly planting the plants.
Owner:SHIHEZI UNIVERSITY

A kind of porcine lactic acid bacillus, probiotics, preparation method and application

The invention relates to a porcine lactobacillus, a microecological preparation and a preparation method and application thereof, belonging to the fields of bioengineering and feed. The biological preservation number of this strain is CCTCC NO: M 2017596, and the classification name is Bacillus lactis Q-21. It is easy to colonize the intestine and grow rapidly, ensuring the quantitative advantage of intestinal beneficial bacteria and preventing the imbalance of intestinal flora. , has the characteristics of adapting to the acidic environment of the gastrointestinal tract, producing spores, and having strong stress resistance. The microecological preparation includes the above-mentioned bacterial species, and the preparation method includes inoculating the above-mentioned bacterial species in a fermentation medium, and fermenting. The preparation method is simple and effective, and can be widely promoted in production and application. The thus-prepared microecological preparation contains highly active swine-derived Bacillus lactis, and when it is applied to piglet breeding, it can reduce the pH value of the gastrointestinal tract of piglets, kill intestinal pathogenic microorganisms, and also promote feed nutrients. digestion and absorption, and improve the growth performance of piglets.
Owner:YATAI XINGYUAN FARMING TECH HAIAN CO LTD +2

Efficient cultivation method for tremella aurantialba basswood

The invention discloses an efficient cultivation method for tremella aurantialba basswood. The efficient cultivation method comprises the steps of pretreatment, inoculation, spawn running, fruiting management and harvesting and specifically comprises the steps that the water content of the basswood is adjusted to be 50% or above, the basswood is cut into sections, and 10%-30% of lime water is smeared on the cut sections to obtain a basswood carrier; blind holes are formed in a basswood carrier according to the hole spacing of 12-20 cm and the line spacing of 5-10 cm, white floccules formed by connecting tremella aurantialba sporocarps and culture materials serve as tremella aurantialba basswood cultivation seeds for inoculation, the culture materials are filled, and then sealing is carried out; the inoculated basswood carriers are stacked and then subjected to spawn running, the piles are turned 4-5 times during spawn running, the temperature is controlled to be 22-25 DEG C, and when the fruiting rate of the basswood carriers reaches 20%-40%, the basswood carriers are moved to a fruiting field for fruiting; in the fruiting period, the canopy density of a fruiting field is controlled to be 0.3-0.6, and the air humidity is controlled to be 80%-90%; and harvesting is carried out after the fruiting bodies of the tremella aurantialba are mature. The tremella auramtialba cultivation period is short, tremella auramtialba growth is fast, about 90-120 days are needed from inoculation to harvesting, the tremella auramtialba growth period is usually started in 2 months, and the tremella auramtialba rich period is started in 4 months.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Preparation method and application of probiotic spore capsid nanomaterial and probiotic composition

The present invention relates to a preparation method and application of a probiotic spore capsid nanomaterial and a probiotic composition, which can effectively solve the problem of inactivation or activity reduction of various gastric acid intolerance probiotics after being taken orally in the stomach, thereby causing colonization in the intestinal tract The problem of low efficiency, as well as the problem of inhibiting the growth and proliferation of harmful bacteria, restoring and rebuilding the intestinal barrier, regulating the intestinal flora, and then realizing the effective prevention and treatment of colitis and colitis-related colon cancer, first, cultivate probiotics, Make it produce spores, then extract the spore capsids through an extruder and prepare capsid nanomaterials (Capsid Nano‑materials, CNMs) with uniform particle size and stable properties, and then grow and cultivate the capsid nanomaterials and probiotics on them Substrate co-incubation to obtain spore capsid nanomaterials and probiotic composition; the preparation method of the invention is simple, the production cost is low and the gastric acid environment is stable, the intestinal flora is adjusted, the intestinal barrier is enhanced, and it is suitable for inflammatory bowel disease and inflammation-induced Cancer treatment is powered by new technologies.
Owner:ZHENGZHOU UNIV
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