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Hypsizygus marmoreus strain medium and preparation method thereof

A technology for culture medium and culture medium formula, which is applied to the field of culture medium and preparation, can solve the problems of high cost of culture medium, complicated production process and high labor cost, and achieve fast colonization, simple preparation method and convenient storage. Effect

Inactive Publication Date: 2016-02-24
天津绿圣蓬源农业科技开发有限公司
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Problems solved by technology

[0003] At present, in factory production, PDA medium is generally used for test tube culture, which is placed in a refrigerator at 4°C for preservation. PDA medium and its preparation method have problems such as high medium cost, complicated production process, and high labor cost. Therefore, There is an urgent need for a simple and effective strain medium and its preparation method, which can be used for a long time under the premise of effectively maintaining the traits of the strain

Method used

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  • Hypsizygus marmoreus strain medium and preparation method thereof

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Embodiment 1

[0012] Culture medium preparation: the weight percentage of the culture medium formula is: wood chips 22%, rice bran 13%, water 65%, wherein the weight percentages of wood chips and rice bran are all dry weight percentages, and poplar wood chips account for 40% of the total dry weight of wood chips , pine wood chips account for 60% of the total dry weight of wood chips. The above-mentioned components are prepared in proportion to the culture medium. First, poplar wood chips, pine wood chips, and rice bran are added to the mixing pot; stir for 30-40 minutes to mix evenly, and then according to Add water according to the water content of the formula, and continue to stir evenly. Medium sterilization: put the prepared medium into culture bottles, select 200 bottles for testing, put the medium into a sterilization device for sterilization, first sterilize at 95°C for 60 minutes, then sterilize at 105°C for 30 minutes Minutes, then sterilized at 121°C for 50 minutes, and finally st...

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Abstract

The invention provides a Hypsizygus marmoreus strain medium and a preparation method thereof. The formula of the Hypsizygus marmoreus strain medium comprises 20-25wt% of wood dust, 10-15wt% of rice bran, and 65wt% of water, wherein the weight percentages of the wood dust and the rice bran are dry weight percentages. The preparation method of the Hypsizygus marmoreus strain medium comprises the processes of mixing a culture material, disinfecting, inoculating, and culturing. The raw materials of the strain medium have the advantages of low price, easy purchasing and convenient storage, and the preparation method of the medium is simple; and Hypsizygus marmoreus strains cultured by adopting the medium have the advantages of short production period, vigorous growth vigor and strong vitality of mycelia, and realization of fast colonization, low pollution rate and difficult ageing after being inoculated to a cultivation material.

Description

technical field [0001] The invention belongs to the technical field of strain culture medium and preparation method, and in particular relates to a strain culture medium of shimeji mushroom and a preparation method thereof. Background technique [0002] The true mushroom is rich in vitamins and a variety of amino acids, among which the content of lysine and arginine is higher than that of common mushrooms, which helps teenagers improve their intelligence, and has the effect of anti-cancer and lowering cholesterol. It has a high value and has become one of the important varieties of industrial production of edible fungi in the country. As an important biological resource, in order to keep its excellent properties unchanged, it should be preserved in time to keep the metabolism of microorganisms at Only in the least active or relatively static state can it not mutate within a certain period of time and maintain its ability to live. [0003] At present, in factory production, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F5/00
CPCC05F5/002Y02A40/20
Inventor 陈启永王旭峰张红颖杨华何乐班立桐黄亮王玉
Owner 天津绿圣蓬源农业科技开发有限公司
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