Grifola frondosa three-stage culture production method

Abstract

The invention provides a grifola frondosa three-stage culture production method. The grifola frondosa three-stage culture production method comprises the steps of first-stage culture production, second-stage culture production, third-stage culture production, culture package production, inoculation and cultivation, bud forcing and mushroom cultivation. The third-stage culture production comprises the steps of obtaining of culture medium recipes, production of the culture media, bagging, sterilization, inoculation, cultivation of the culture and raising of the culture. The grifola frondosa three-stage culture production method is characterized in that the culture medium recipes include the wood strip culture medium recipe and the wheat grain culture medium recipe, and the production of the culture media comprises production of a wood strip culture medium and production of a wheat grain culture medium. Compared with a wood chip culture, the culture obtained through the grifola frondosa three-stage culture production method has the advantages that inoculation efficiency is 5-8 percent higher, the maximum culture growth distance is 50-100 percent shorter, the cultivation efficiency is 20-60 percent higher, each culture bag is consistent in mycelial age vertically, so that nutrients are released in a centralized mode, and the first-batch mushroom yield can be increased by 15-30 percent. Compared with liquid culture, the culture obtained through the grifola frondosa three-stage culture production method is applicable to existing culture production and detection, inoculation equipment and technicians in China, and can reduce the cultivation infection rate by 2-5 percent.

Description

technical field [0001] The invention belongs to the technical field of Grifola frondosa strains, in particular to a preparation method for the three-stage strains of Grifola frondosa. Background technique [0002] Grifola frondosa (Dicks.ex Fr) S. F. Gray], also known as Maitake, belongs to Basidiomycotina, Phytomycetes, Phyllomycetes, Polyporaceae, Arboria. It is a rare economic species recommended by WHO and FAO to developing countries, and it is a high-grade rare edible fungus with both food and medicine. [0003] my country began to artificially domesticate and cultivate Grifola frondosa from the early 1980s. At present, there are Hebei and Zhejiang provinces with large scale of cultivation. Other provinces such as Liaoning, Shandong, Shanxi, Shaanxi, Anwei, Jiangsu, Hubei, Fujian, etc. also have ash frondosa. The artificial cultivation of tree flowers, but not on a large scale. The industrial production technology research of Grifola frondosa started late in my country...

AI Technical Summary

Problems solved by technology

[0009] In view of the low inoculation efficiency and slow growth rate (the maximum growth distance of the strain) and liquid strains of the wood chip solid strains used in the production of Grifola frondosa, it is necessary to select seed production, testing and inoculation equipment and professional testing and inoculation personnel etc., the present invention provides a method for making the three-grade strain of Grifola frondosa that is suitable for the production of Grifola frondosa at the present stage in my country