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Preparation method and application of probiotic spore Capsid Nano-materials and probiotic composition

A technology of nanomaterials and probiotics, applied in the field of pharmaceutical preparations, can solve the problems of inactivation, reduction, and low efficiency of intestinal colonization, and achieve the effects of regulating intestinal flora, stable gastric acid environment, rapid colonization and proliferation

Active Publication Date: 2021-03-05
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a preparation method and application based on probiotic spore capsid nanomaterials and probiotic composition, which can effectively solve the probiotics of various gastric acid intolerance. Bacteria are inactivated or reduced in activity after being taken orally in the stomach, resulting in the problem of low colonization efficiency in the intestinal tract, as well as inhibiting the growth and proliferation of harmful bacteria, restoring and rebuilding the intestinal barrier, and regulating the intestinal flora, thereby realizing the treatment of colitis and Effective prevention and treatment of colitis-associated colon cancer

Method used

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  • Preparation method and application of probiotic spore Capsid Nano-materials and probiotic composition

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Effect test

Embodiment 1

[0019] In the specific implementation of the present invention, a preparation method based on probiotic spore capsid nanomaterial and probiotic composition comprises the following steps:

[0020] (1) Cultivation of probiotics: inoculate the lyophilized powder of probiotic Bacillus in the nutrient gravy liquid medium, place it in a constant temperature incubator at 37°C, and cultivate it at a shaking speed of 140r / min. During the cultivation process, add 0.005% MnSO by volume in nutrient broth broth 4 ·H 2 O, promote probiotics to produce spores, make them recover and grow and proliferate. When the bacteria proliferate to the logarithmic phase, collect the bacterial liquid and set it aside;

[0021] (2) Extraction of spores: Inoculate the bacterial solution prepared in step (1) into the nutrient gravy liquid medium according to the inoculation amount of 3% of the volume of the nutrient gravy liquid medium, and place it in a constant temperature incubator at 37°C. To continue ...

Embodiment 2

[0025] In the specific implementation of the present invention, a preparation method based on probiotic spore capsid nanomaterial and probiotic composition comprises the following steps:

[0026] (1) Cultivate probiotics: Inoculate the lyophilized powder of probiotics Bifidobacterium in nutrient gravy liquid medium, place them in a constant temperature incubator at 37°C, and cultivate them at a shaking speed of 130r / min. During the cultivation process Add 0.004% MnSO by volume to the nutrient gravy broth 4 ·H 2 O, promote probiotics to produce spores, make them recover and grow and proliferate. When the bacteria proliferate to the logarithmic phase, collect the bacterial liquid and set it aside;

[0027](2) Extraction of spores: Inoculate the bacterial solution prepared in step (1) into the nutrient gravy liquid medium according to the inoculation amount of 2% of the volume of the nutrient gravy liquid medium, and place it in a constant temperature incubator at 37°C. To cont...

Embodiment 3

[0031] In the specific implementation of the present invention, a preparation method based on probiotic spore capsid nanomaterial and probiotic composition comprises the following steps:

[0032] (1) Cultivate probiotics: Inoculate the lyophilized powder of probiotic Lactobacillus in nutrient gravy liquid medium, place it in a constant temperature incubator at 37°C, and cultivate it at a shaking speed of 160r / min. During the cultivation process, add 0.006% MnSO by volume of nutrient broth broth 4 ·H 2 O, promote probiotics to produce spores, make them recover and grow and proliferate. When the bacteria proliferate to the logarithmic phase, collect the bacterial liquid and set it aside;

[0033] (2) Extraction of spores: Inoculate the bacterial liquid prepared in step (1) into the nutrient gravy liquid medium according to the inoculum volume of 4% of the volume of the nutrient gravy liquid medium, and place it in a constant temperature incubator at 37°C. To continue culturing...

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Abstract

The invention relates to a preparation method and application of a probiotic spore Capsid Nano-materials and probiotic composition. The problem that various gastric acid-intolerant probiotics are inactivated or reduced in activity after being orally taken through the stomach, and then the intestinal colonization efficiency is low can be effectively solved, growth and proliferation of harmful bacteria are inhibited, the intestinal barrier is recovered and rebuilt, the intestinal flora is adjusted, and then the colon cancer related to colitis and colitis is effectively prevented and treated. Thepreparation method comprises the steps that firstly, probiotics are cultured to generate spores, then spore Capsid is extracted through an extruder and prepared into the Capsid Nano-materials (CNMs)uniform in particle size and stable in property, then the Capsid Nano-materials and the probiotics are co-incubated in a growth culture medium, and the spore Capsid Nano-materials and probiotic composition is obtained. The preparation method is simple, low in production cost and stable in gastric acid environment, the intestinal flora is adjusted, the intestinal barrier is enhanced, and a new technical support is provided for treatment of inflammatory bowel diseases and inflammation induced cancers.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, in particular to a preparation method and application of a probiotic spore capsid nanomaterial and a probiotic composition. Background technique [0002] In recent years, probiotic therapy has been widely used in the clinical treatment of various diseases due to its significant advantages in regulating the balance between gut microbes, the environment, and the immune system. It is difficult for most probiotics to survive in the extreme gastric acid environment after oral administration. Therefore, the number of live bacteria that can successfully reach the intestinal colonization and play a role cannot be guaranteed, which limits the use of most probiotics in oral drug delivery systems Applications. As a class of probiotics that can produce spores, Bacillus can not only promote intestinal digestion, but also be used to treat intestinal inflammation, regulate host symbiotic flora and in...

Claims

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Application Information

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IPC IPC(8): A61K35/742A61K35/745A61K35/747A61K47/46A61P31/04A61P1/00A61P35/00A61P29/00C12N1/20C12N1/16C12N1/04C12R1/01C12R1/07C12R1/225C12R1/145
CPCA61K35/742A61K35/745A61K35/747A61K47/46A61P1/00A61P29/00A61P31/04A61P35/00C12N1/04C12N1/16C12N1/20
Inventor 王蕾宋庆龄张云张振中高慧王珂郑翠霞赵洪娟冯倩华张红岭
Owner ZHENGZHOU UNIV
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