Preservation method of hypsizigus marmoreus strain

Abstract

The invention provides a preservation method of a hypsizigus marmoreus strain. The method comprises a culture medium mixing process, a culture medium sterilization process, a strain inoculation process, a strain culture process and a strain subculture process, wherein the strain subculture process is the process for carrying out primary subculture in the culture medium at every 30-35 days; the strain culture medium is prepared from the following raw materials in percentage by weight: 20%-25% of sawdust, 10%-15% of rice bran and 65% of water, wherein the percentage by weight of the sawdust and the rice bran is the percentage by dry weight. According to the preservation method provided by the invention, the raw material for the culture medium is low in price, easy to purchase, convenient to store, long in strain preservation period and stable in strain character; the strain preserved by the preservation method can be continuously used for 10-20 years.

Description

technical field [0001] The invention belongs to the technical field of strain preservation, and in particular relates to a method for preserving the strains of shiitake mushrooms. Background technique [0002] Shiji mushroom is rich in vitamins and various amino acids, among which the content of lysine and arginine is higher than that of ordinary mushrooms, which helps to increase the intelligence of young people, and has anti-cancer and cholesterol-lowering effects. It has high value and has become one of the important varieties of edible fungi in the country. As an important biological resource, in order to keep its excellent properties unchanged, it should be preserved in time, so that the metabolism of microorganisms is in the The most inactive or relatively static state can keep it alive for a certain period of time without mutating. [0003] The preservation methods of edible mushrooms mainly include liquid nitrogen cryopreservation method, inclined plane regular tran...

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Problems solved by technology

[0003] The preservation methods of edible fungus strains mainly include liquid nitrogen cryopreservation method, slope regular transplantation method, liquid paraffin method, sand tube preservation method and natural substrate treasure method, etc. Liquid nitrogen cryopreservation method is a relatively advanced method at present, but the cost It is relatively high and cannot be widely used in industrial production. At present, in industrial production, PDA medium is generally used for test tube culture, placed in a refrigerator at 4°C, and subcultured every 90 days. The period is generally 1-2 years, and there are problems such as high cost of culture medium, long cultivation cycle, poor quality, and high pollution rate. Therefore, there is an urgent need for a simple and effective method for strain preservation. Can also be used for a longer time

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