Anthraquinone compounds, preparation method and application

A compound and anthraquinone technology, applied in the field of anthraquinone compound preparation, can solve the problems of no marine fungal secondary metabolites for preventing and controlling plant bacterial diseases, less application, etc., achieve excellent bactericidal performance, not limited by resources, preparation Efficient effect

Active Publication Date: 2017-12-15
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, most of the current studies on the activity of secondary metabolites of marine fungi focus on human medicine, and there are few applications in agriculture, especially in the control of plant pathogenic bacteria. Metabolites Controlling Plant Bacterial Diseases Report

Method used

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  • Anthraquinone compounds, preparation method and application
  • Anthraquinone compounds, preparation method and application
  • Anthraquinone compounds, preparation method and application

Examples

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preparation example Construction

[0041] Another embodiment of the present invention provides a method for preparing anthraquinone compounds, comprising the following steps:

[0042] The marine fungus Fusarium equiseti GLY27 was cultured in the strain culture medium, and then transferred to the fermentation medium for fermentation;

[0043] Soak the obtained fermented product in ethyl acetate, then soak it in a solution with a volume ratio of methanol:dichloromethane of 1:1, concentrate under reduced pressure, filter, extract the remaining water phase with ethyl acetate, concentrate the extract under reduced pressure, and dry After the crude extract;

[0044] The crude extract is subjected to chromatography, elution, and concentration to obtain the compound described in formula (I).

[0045] In this example, marine fungi can produce metabolites after strain activation and fermentation expansion, which is beneficial to the preparation of extracts. After fermentation, the fermentation products are soaked in eth...

Embodiment 1

[0056] The cultivation and fermentation of embodiment 1 marine fungus Fusarium equiseti GLY27

[0057] The strain culture of marine fungus Fusarium equiseti GLY27 adopts potato dextrose agar medium, which contains potato 6g / L, glucose 20g / L, agar 20g / L, and the rest is water. 5-7 days;

[0058] (2) Fermentation culture of marine fungus Fusarium equiseti GLY27 Use rice solid medium, add rice 40g, water 30mL to each 500mL Erlenmeyer flask, ferment 3 bottles, and culture the fungal strain at 28°C for 30-40 days.

Embodiment 2

[0059] The preparation of embodiment 2 anthraquinone compounds

[0060] Soak the fermented product obtained twice in ethyl acetate, then soak in a solution with a volume ratio of methanol:dichloromethane of 1:1, concentrate under reduced pressure, filter, extract the remaining aqueous phase with ethyl acetate, and concentrate the extract under reduced pressure , to obtain the crude extract after drying;

[0061] The crude extract was subjected to vacuum silica gel column chromatography, the stationary phase was 100-200 mesh normal phase silica gel, the mobile phase was eluted with 10% ethyl acetate-petroleum ether mixed solvent, and then eluted with 100% ethyl acetate. After the 100% ethyl acetate eluate was concentrated, ODS (48-63 μ m) reversed-phase silica gel column chromatography was carried out, and the mobile phase was 30%, 40%, 50%, 60%, 70%, 80% aqueous methanol, 50 After concentration of the % methanol eluate, Sephadex LH-20 gel column chromatography was carried out...

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Abstract

The invention provides anthraquinone compounds, a preparation method and an application, and belongs to the field of compounds. The anthraquinone compounds have the activity of resisting pathogenic bacteria of plants, and can be used for preventing and treating plant diseases caused by the pathogenic bacteria of the plants when applied as bactericides. The anthraquinone compounds are extracted from marine fungus Fusarium equiseti GLY27 with the collection number being CGMCC (China General Microbiological Culture Collection Center) 14348. The anthraquinone compounds are used for preventing and treating the plant diseases caused by the pathogenic bacteria of the plants, wherein the pathogenic bacteria of the plants comprise P.syringae, A.avenae and E.carotovora.

Description

technical field [0001] The invention relates to the field of compounds, in particular to an anthraquinone compound, a preparation method and an application. Background technique [0002] Plant diseases seriously endanger agricultural production and cause huge economic losses every year. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the average yield loss of crops due to diseases is 10% to 15% of the total output every year. Among them, the problems caused by plant bacterial diseases are particularly serious. With the discontinuation of agricultural streptomycin sulfate, there is a lack of effective control agents, while copper preparations and other control effects are general, and they are chemical pesticides, which are likely to cause environmental pollution and food safety. problems, and the emergence of antibiotic resistance in pathogenic bacteria. Therefore, it is an urgent task to develop new environmentally friendl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C50/18C12P7/66A01N35/06A01P1/00C12R1/77
CPCA01N35/06C07C50/18C12P7/66
Inventor 赵栋霖张成省李义强尤祥伟邹平徐茜陈志厚孟晨袁源徐辰生林伟荆常亮廖锦建徐宗昌
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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