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Marine fungi-derived azaphilones compound and preparation method and application thereof

A technology for marine fungi and compounds, applied in the fields of botanical equipment and methods, biochemical equipment and methods, fungi, etc., can solve problems such as limited activity against agricultural pathogenic fungi, and achieve good inhibitory activity and broad application prospects.

Active Publication Date: 2019-05-03
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] One of the purposes of the present invention is to provide a kind of azaphilones compound derived from marine fungi and its preparation method, to solve the problem of limited activity of azaphilones compounds derived from marine fungi and the limited activity against agricultural pathogenic fungi in the prior art

Method used

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  • Marine fungi-derived azaphilones compound and preparation method and application thereof
  • Marine fungi-derived azaphilones compound and preparation method and application thereof
  • Marine fungi-derived azaphilones compound and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0051] (1) Cultivation of the marine fungus HBU-135 strain

[0052] The medium used for the culture of marine fungus HBU-135 contains 1.0wt% glucose, 0.1wt% yeast extract, 0.2wt% peptone, 1.0wt% agar, 3.0wt% coarse sea salt, and the rest is water. It is made into a test tube when used For slant, the marine fungus strain HBU-135 was grown at 28°C for 3 days.

[0053] (2) Fermentation of marine fungus HBU-135

[0054] The fermentation medium used for the fermentation culture of the marine fungus HBU-135 is that each 1000mL Erlenmeyer flask contains 80g of potatoes (boiled in water for 20 minutes to remove potato dregs), 20g of glucose, MgCl 2 13g, 400mL of water, and the strain was fermented and cultured at 28°C for 45 days to obtain a fermented product; a total of 200 1000mL Erlenmeyer flasks were used for fermentation.

[0055] (3) Separation analysis of Azaphilones class

[0056] Take the fermented product obtained in step (2), extract 3 times with ethyl acetate, combine ...

Embodiment 2

[0061] (1) Cultivation of the marine fungus HBU-135 strain

[0062] The medium used for the culture of the marine fungus HBU-135 contains 10wt% of glucose, 4.0wt% of yeast extract, 4.0wt% of peptone, 6.0wt% of agar, 10wt% of coarse sea salt, and the rest is water. Fungal strains were grown at 35°C for 5 days.

[0063] (2) Fermentation of marine fungus HBU-135

[0064] The fermentation medium used for the fermentation culture of the marine fungus HBU-135 is that each 1000mL Erlenmeyer flask contains 120g of potatoes (boiled in water for 20 minutes to remove the potato dregs), 30g of glucose, MgCl 2 20g, 600mL of water; the fermentation culture condition is static culture at 35°C for 50 days to obtain the fermented product.

[0065] (3) Separation and analysis of Azaphilones compounds

[0066]Take the fermented product obtained in step (2), extract 4 times with ethyl acetate, combine the ethyl acetate extracts and concentrate under reduced pressure to obtain a crude extract,...

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Abstract

The invention provides a marine fungi-derived azaphilones compound, a preparation method thereof and application thereof in preparing a medicine for preventing agricultural pathogenic fungi, the marine fungi is Pleosporales sp. HBU-135 strain, the preservation date is October 18th, 2018, and the preservation number is CGMCC No. 16379, the preservation unit is the General Microbiology Center of theChina Microbial Culture Collection Management Committee, and the preservation address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. The structural formula of the compound is as shownin a formula 1 or a formula 2. The Pleosporales sp. HBU-135 strain is cultured by fermentation, and the above compound is isolated from the fermentation product. Through the verification, the compound has strong inhibitory activity against various agricultural pathogenic fungi, can be prepared into an antibacterial agent, and has broad application prospects.

Description

technical field [0001] The invention relates to the field of marine natural medicinal chemistry, in particular to a marine fungus-derived azaphilones compound, a preparation method and application thereof. Background technique [0002] In recent years, with global warming and changes in farming systems and methods, the problem of infectious diseases of crops has spread and expanded, bringing significant economic losses to human agricultural development. Among the infectious diseases of crops, there are mainly two kinds of fungal diseases and bacterial diseases, among which fungal diseases account for about 80% of the diseases. For a long time, chemical fungicides have been the main means of preventing and controlling agricultural fungal diseases, and chemical pesticides not only bring environmental pollution and potential harm to human health, but also bring about the impact of non-target organisms and the enhancement of plant pathogenic bacteria resistance And other proble...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/06C07D311/76A01N43/16A01P3/00C12R1/645
Inventor 曹飞朱华结刘云凤李龙飞
Owner HEBEI UNIVERSITY
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