Marine fungi aspergillus terreus C23-3, fermentation liquor active extract as well as preparation method and application thereof

A technology for marine fungi and Aspergillus terreus, applied in the field of microorganisms, can solve the problems of high price, large toxic and side effects of drugs, and achieve the effects of anti-oxidation and inhibition of acetylcholinesterase activity, and good application prospects.

Inactive Publication Date: 2018-06-15
SHENZHEN INST OF GUANGDONG OCEAN UNIV +1
View PDF2 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects and deficiencies of existing drugs for treating senile dementia, such as high toxicity and high price, and provide a kind of marine fungus Aspergillus terreus Aspergillus terreus C23-3

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Isolation and Identification of Marine Fungus Aspergillus terreus C23-3

[0061] 1. Isolation method of strain

[0062] (1) Sample: peony coral in the South China Sea.

[0063] (2) Separation method

[0064] Wash the coral 3 times with sterile seawater, grind it to a muddy state with a sterilized mortar and pestle at room temperature and aseptic environment, take a little (about 1g) into 9 mL with a sterilized medicine spoon In sterile seawater, add glass beads to shake for 1 min, then let it stand for 20 min, and take the supernatant to make 10 -1 、10 -2 、10 -3 、10 -4 1-fold dilution, respectively draw 10 -1 、10 -2 、10 -3 、10 -4 Put 100 μL of the diluted sample dilution on the corresponding separation medium plate, spread evenly on the surface of the corresponding medium with a sterile glass coating rod, and culture in a constant temperature incubator at 28°C for 3 to 5 days. Then use a sterile razor blade to cut off the tip of the mycelium from the...

Embodiment 2

[0075] Example 2 Activity test of total extract from fermentation of marine fungus Aspergillus terreus C23-3

[0076] 1. Obtaining the total extract from fermentation of Aspergillus terreus C23-3

[0077] (1) Seed culture: Inoculate the strains from the slope onto a sterile seed solid medium plate, place the culture plate in a constant temperature (28°C) constant humidity (70%) incubator upside down for culture, and the culture time 4 to 5 days;

[0078] (2) Expansion culture: fill 1L of liquid medium into a 3L Erlenmeyer flask, seal it with a gas-permeable sealing film, and then sterilize in an autoclave at 121°C and 0.1 Mpa for 20 minutes; after cooling, inoculate the above-mentioned Step (1) After cultivation, the seed flat plate bacteria-carrying medium block is 30-40 cm 2 The proportion of inoculation, sealing, placed in a constant temperature of 28 ℃ indoor culture, natural light, culture time is 21 to 24 days, to obtain fermentation broth.

[0079] Wherein, the cultu...

Embodiment 3

[0092] Example 3 Extraction of Active Substances from Marine Fungus Aspergillus terreus C23-3 Fermentation Broth

[0093] 1. Medium

[0094] (1) Seed solid medium: add 20.0g sucrose, 20.0g sea salt, 5g peptone and 15.0g agar to every 500mL potato juice, pH is 7.4±0.2;

[0095] (2) The liquid medium is: add 20.0g sucrose, 20.0g sea salt and 5g peptone to every 500mL potato juice, the pH is 7.4±0.2;

[0096] Among them, potato juice: take fresh potatoes, peeled and cut into 1cm 3 For every 200g of potato pieces, add 500 mL of distilled water and boil for 20 minutes, filter through double-layered gauze cloth, and dilute to 500 mL with distilled water.

[0097] 2. Extraction method

[0098] (1) Seed culture: Inoculate the strains from the slope onto a sterile seed solid medium plate, place the culture plate in a constant temperature (28°C) constant humidity (70%) incubator upside down for culture, and the culture time 4 to 5 days;

[0099] (2) Expansion culture: fill 1L of li...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to marine fungi aspergillus terreus C23-3, a fermentation liquor active extract as well as a preparation method and application thereof. The marine fungi aspergillus terreus C23-3 is collected in the Guangdong Microbiological Culture Collection Center (GDMCC) on Jan 22th, 2018 with the collection number GDMCC No. 60316. Active substances of standing fermentation liquor of theaspergillus terreus C23-3 are subjected to extraction and purification; a fermentation liquor total extract of a bacterial strain can well resist oxidization and restrain the activity of acetylcholinesterase; an active extract prepared by purifying the fermentation liquor of the bacterial strain has very high oxidization resistance and/or acetylcholin esterase restraining activity, and has a very good application prospect on the aspect of preparation of an anti-senile dementia medicament and on the aspect of preparation of an anti-oxidization and/or acetylcholin esterase activity restrainingmedicament.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a marine fungus Aspergillus terreus C23-3, an active extract from fermentation broth, a preparation method and application thereof. Background technique [0002] The current research results show that the occurrence of Alzheimer's disease is closely related to nerve damage and the loss of acetylcholine, and the hallmark lesions of AD, such as β-amyloid (Aβ) and phosphorylated tubulin (Tau), can stimulate The generation of reactive oxygen species (ROS) causes oxidative stress, damages neurons, and causes cognitive dysfunction. The continuous activation of oxidative stress can further promote the aggregation and deposition of Aβ and phosphorylated Tau, mitochondrial damage, lead to the occurrence of AD and accelerate its pathological process. Antioxidants can clear the oxides in the brain and prevent the formation of oxides, inhibit the activity of prooxidative enzymes, neutraliz...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/06A61P39/06A61P25/28C12R1/66
CPCA61K36/06C12N1/145C12R2001/66
Inventor 张翼聂影影雷晓凌杨文聪杨静明洪鹏志冯妍宋采
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap